Digestive proteases and in vitro protein digestibility of feed ingredients for the Central American river turtle, <i>Dermatemys mawii</i>

Rangel-Mendoza, Judith A.; Hernández-García, Jorge; Álvarez‐González, Carlos Alfonso; Guerrero‐Zárate, Rocío; Zenteno-Ruiz, Claudia Elena; López-Luna, Marco A.

doi:10.1111/jpn.12889

Cited by 4 publications

(2 citation statements)

References 41 publications

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“…In Fig. 5, the results of SDS-PAGE revealed six bands with stomach after feeding in other reptile species such as white turtle (Dermatemys mawii) and the red-eared turtle (Trachemys scripta elegans) that show optimum acid protease activity at pH 2 and pH 2.5, respectively (Rangel-Mendoza et al, 2018;Sun et al, 2007).…”

Section: Resultsmentioning

confidence: 99%

“…This assumption is corroborated by the optimum activity obtained for trypsin and leucine aminopeptidase, which shows the highest activity at pH 10 and pH 8, respectively. Studies in reptiles such as the white turtle (Dermatemys mawii), show optimum alkaline protease activity at pH 9 (Rangel-Mendoza et al, 2018), while the redeared turtle (Trachemys scripta elegans) shows optimum pH in pancreas, and foregut, midgut, and hindgut of 8.0, 7.0, 8.0, and 8.5, respectively (Sun et al, 2007). Tracy et al, (2015), reported the digestive morphology, the activity of membrane enzymes (aminopeptidases, maltase and sucrase) and the absorption of nutrients in Alligator mississippiensis and Crocodylus porosus, where it was found that the villi of the proximal intestine varied in the two species, having A. misisipiensis the highest absorption and aminopeptidase activity compared to C. porosus.…”

Section: Discussionmentioning

confidence: 99%

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Digestive proteases of Morelet’s crocodile (Crocodylus moreletii) in three life stages

Castillo-Rodríguez,

Rangel-Mendoza,

Peña-Marín

et al. 2024

Rev. Biol. Trop.

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Introduction: Morelet’s crocodile (Crocodylus moreletii) is a species distributed in the Mexican southeast and threatened due to multiple pressures. Objective: To characterize the digestive proteases in the acid phase (stomach) and alkaline phase (intestine) of three life stages of C. moreletii in captivity (hatchling, juvenile, and adult). Methods: Total alkaline and acid protease activities were quantified using casein and haemoglobin as substrates. Trypsin, chymotrypsin, leucine aminopeptidase, and elastase activities were quantified using synthetic substrates. Protease profiles were analysed by SDS-PAGE and Native-PAGE. Results: The specific activity of acid and alkaline proteases showed differences between the three stages, finding the highest activity in the juveniles. Trypsin, chymotrypsin, leucine aminopeptidase, and elastase activities were higher in hatchlings. There were differences in optimum pH and temperature of acid and alkaline proteases, trypsin, and leucine aminopeptidase between the three stages, demonstrating the diversification of the enzymes according to different stages, as well as the presence of specific isoforms in each stage of C. moreletii. The acid phase zymogram showed four bands with pepsin-like acid activity in the hatchling and juvenile crocodile, while in the adult only two of the four bands were detected. The alkaline zymogram showed that the hatchling had the highest number of activity bands compared to the other stages, corresponding to the high specific activity reported in the alkaline phase. Conclusions: Digestive proteases of Morelet’s crocodile differ in their biochemical characteristics and the number of proteases between hatchling, juvenile, and adult. This could help in the future design of balanced diets as well to the sustainable management and production of this species.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Digestive proteases of Morelet’s crocodile (Crocodylus moreletii) in three life stages

Castillo-Rodríguez,

Rangel-Mendoza,

Peña-Marín

et al. 2024

Rev. Biol. Trop.

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Genetic diversity and population structure of founders from wildlife conservation management units and wild populations of critically endangered Dermatemys mawii

Gallardo-Alvarez

Lesher-Gordillo

Machkour‐M’Rabet

et al. 2019

Global Ecology and Conservation

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Differential digestive and metabolic profile of juveniles and adults of the estuarine-dependent marine fish Mugil liza (Mugilidae) cohabiting inside a southwestern Atlantic coastal lagoon

2023

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Integrative studies on intraspecific differences of digestive enzymes, energy reserves and morphological/somatic traits in juveniles and adults of estuarine-dependent marine fish while inhabiting inside a coastal lagoon are lacking. This study was carried on juveniles and adults of Mugil liza (Valenciennes, 1836) inhabiting inside Mar Chiquita Coastal Lagoon (Argentina). Both stages exhibited amylase, maltase, sucrase, lipase, trypsin and aminopeptidase-N (APN) activities in the intestine, which were active over a wide range of pH and temperature and exhibited Michaelis-Menten kinetics. Adults exhibited higher intestinal coefficient (31%), higher amylase (32%) and lower maltase (82%) and lipase activities (38%). Glycogen concentration in liver and muscle was similar while free glucose concentration was higher in adults (772% and 400% respectively). In adults retroperitoneal fat (absent in juveniles) constituted the main triglycerides storage site, while triglycerides concentration in liver and muscle was lower (86% and 80% respectively) than in juveniles. No differences were found in protein concentration in any storage organ. The results show that juveniles and adults exhibit a digestive and metabolic profile at the biochemical level and intraspecific differences in key components of carbohydrate and lipid metabolism that could support preparation for reproductive migration of adults outside the coastal lagoon.

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Digestive proteases and in vitro protein digestibility of feed ingredients for the Central American river turtle, Dermatemys mawii

Cited by 4 publications

References 41 publications

Digestive proteases of Morelet’s crocodile (Crocodylus moreletii) in three life stages

Digestive proteases of Morelet’s crocodile (Crocodylus moreletii) in three life stages

Genetic diversity and population structure of founders from wildlife conservation management units and wild populations of critically endangered Dermatemys mawii

Differential digestive and metabolic profile of juveniles and adults of the estuarine-dependent marine fish Mugil liza (Mugilidae) cohabiting inside a southwestern Atlantic coastal lagoon

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