Digging deeper into the plant cell wall proteome

Lee, Sangjik; Saravanan, Ramu S.; Damasceno, C. M. B.; Yamane, Hisayo; Kim, Byung-Dong; Rose, Jocelyn K. C.

doi:10.1016/j.plaphy.2004.10.014

Cited by 94 publications

(80 citation statements)

References 88 publications

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“…Although we used GCPs as starting material, 29 of the identified GC proteins were identified by a previous cell wall proteomic study (Bayer et al, 2006): these proteins may localize to multiple subcellular compartments, including both apoplastic and symplastic destinations, or be present in secretory vesicles that have not yet fused with the plasma membrane (Lee et al, 2004). Indeed, further GO analysis predicted that 23 of the 29 (79%) proteins also localize to non-cell wall subcellular compartments.…”

Section: Comparison Of the Gc Proteome With Other Proteomesmentioning

confidence: 99%

Functional Proteomics ofArabidopsis thalianaGuard Cells Uncovers New Stomatal Signaling Pathways

et al. 2008

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We isolated a total of 3 3 10 8 guard cell protoplasts from 22,000 Arabidopsis thaliana plants and identified 1734 unique proteins using three complementary proteomic methods: protein spot identification from broad and narrow pH range twodimensional (2D) gels, and 2D liquid chromatography-matrix assisted laser desorption/ionization multidimensional protein identification technology. This extensive single-cell-type proteome includes 336 proteins not previously represented in transcriptome analyses of guard cells and 52 proteins classified as signaling proteins by Gene Ontology analysis, of which only two have been previously assessed in the context of guard cell function. THIOGLUCOSIDE GLUCOHYDROLASE1 (TGG1), a myrosinase that catalyzes the production of toxic isothiocyanates from glucosinolates, showed striking abundance in the guard cell proteome. tgg1 mutants were hyposensitive to abscisic acid (ABA) inhibition of guard cell inward K + channels and stomatal opening, revealing that the glucosinolate-myrosinase system, previously identified as a defense against biotic invaders, is required for key ABA responses of guard cells. Our results also suggest a mechanism whereby exposure to abiotic stresses may enhance plant defense against subsequent biotic stressors and exemplify how enhanced knowledge of the signaling networks of a specific cell type can be gained by proteomics approaches.

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Section: Comparison Of the Gc Proteome With Other Proteomesmentioning

confidence: 99%

Functional Proteomics ofArabidopsis thalianaGuard Cells Uncovers New Stomatal Signaling Pathways

et al. 2008

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“…Analyses of protein populations from the walls of many plant organs and tissues have been reported (for review, see Lee et al, 2004;Jamet et al, 2006Jamet et al, , 2008a, together with detailed methodologies to optimize extraction (Feiz et al, 2006;Watson and Sumner, 2007;Jamet et al, 2008b). These have resulted in catalogs of proteins whose identity matches known functions associated with wall-related processes, such as polysaccharide modification, defense, and signaling, as well as many with unknown functions.…”

Section: Who Belongs To the Cell Wall Proteome?mentioning

confidence: 99%

“…Another category of unexpected cell wall-localized proteins has been suggested by the identification of secreted proteins through individual protein localization studies, or functional screens (Zhu et al, 1994;Lee et al, 2004Lee et al, , 2006Kim et al, 2008;Cheng et al, 2009), which do not apparently have targeting sequences that would direct to them to the classical secretory pathway. In the next section we discuss the implications for such observations for compiling a more complete compendium of the cell wall proteome.…”

Section: Who Belongs To the Cell Wall Proteome?mentioning

confidence: 99%

Straying off the Highway: Trafficking of Secreted Plant Proteins and Complexity in the Plant Cell Wall Proteome

2010

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From simply skimming through the abstract lists of more or less any collection of both basic and applied plant-related journals, it is immediately apparent that the plant cell wall represents a nexus of many fields of research: growth and development, plant-pathogen interactions, abiotic stress, self-and interorganismal recognition, signaling systems, numerous primary and specialized metabolic processes, biomaterials and bioproducts, and many others. That said, and to deal with an issue of semantics, while the term cell wall can refer specifically to the structural matrix that surrounds all plant cells, for the purposes of this Update it is used more broadly, also to include the apoplast, or extracellular environment. Given its multifunctional nature then, it is not surprising that the apoplast houses a dynamic and complex proteome, and the compendium of cell wall proteins continues to grow as researchers from disparate disciplines discover new roles for extracellular proteins. In addition, however, as cell wall proteomics projects develop and the subcellular localizations of an ever-growing list of plant proteins are determined, a number of surprises have been thrown up, both in terms of the identity of secreted proteins and the trafficking pathways that they follow. The purpose of this Update is to give some examples of previously unsuspected aspects of plant cell wall protein trafficking that are challenging longheld assumptions, rather than to provide an exhaustive review, and to highlight some questions that can be categorized into the "who, how, where, and when" of the cell wall proteome.

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“…Proteomics attempt not only to give a larger vision of the proteins present in a particular organ at a given stage of development, but also deal with some of these issues. Several recent reviews on plant proteomics have described the available methods in this area [24][25][26], and the application of proteomics to the study of cell walls [27,28].…”

Section: Towards a More Comprehensive View Of Cell Wall Proteinsmentioning

confidence: 99%

Cell wall proteins: a new insight through proteomics

Jamet

Canut

Boudart

et al. 2006

Trends in Plant Science

255

211

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Cell wall proteins (CWP) are essential constituents of plant cell walls involved in modifications of cell wall components, wall structure, signaling, and interactions with plasma membrane proteins at the cell surface. The application of proteomic approaches to the cell wall compartment raises important questions: Are there technical problems specific to cell wall proteomics? What kinds of proteins can be found in Arabidopsis walls? Are some of them unexpected? What sort of post-translational modifications have so far been characterized in CWP? The purpose of this review is to discuss the experimental results obtained so far using protomics, as well as some of the new questions challenging future research. Glossary AGP (arabinogalactan proteins):cell wall highly glycosylated HRGP that contain repetitive motifs such as (Ser, Thr, Ala)-Hyp-(Ser, Thr, Ala)-Hyp or (Ser, Thr, Ala)-Hyp-Hyp. AG (arabinogalactan)-peptides: AGP that have a predicted mature protein backbone of 10 to 13 amino acid residues. BBE: berberine-bridge (S)-reticulin:oxygen oxidoreductases. CWP: cell wall proteins. Extensins: cell wall structural HRGP, with numerous Ser-Hyp n (n≥3) motifs separated by Tyr-, Lys-, His-and Val-rich regions. GAP (glycosylphosphatidylinositol anchored proteins): proteins that are lipid-anchored to the external phase of the plasma membrane. GH (glycoside hydrolases): enzymes that hydrolyze the glycosidic bond between two carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. HRGP: hydroxyproline-rich glycoproteins, i.e. extensins and AGP. Lectins: structurally diverse proteins that bind to specific carbohydrates. LRR (leucine-rich repeat): short sequence motifs having diverse functions and cellular locations, usually involved in protein-protein interactions. PME (pectin methylesterases): enzymes that catalyze the de-esterification of pectin into pectate and methanol. Proteome: the complete profile of proteins expressed, at a given time and environmental conditions, in a given organ, tissue, or cell. PRP (proline-rich proteins): structural CWP rich in proline residues. Transcriptome: the complete collection of transcribed elements of the genome.

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Digging deeper into the plant cell wall proteome

Cited by 94 publications

References 88 publications

Functional Proteomics ofArabidopsis thalianaGuard Cells Uncovers New Stomatal Signaling Pathways

Functional Proteomics ofArabidopsis thalianaGuard Cells Uncovers New Stomatal Signaling Pathways

Straying off the Highway: Trafficking of Secreted Plant Proteins and Complexity in the Plant Cell Wall Proteome

Cell wall proteins: a new insight through proteomics

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