The practice of preparing e-portfolio as part of job application skills is ubiquitous nowadays. Hence, the university undergraduates need to be trained to incorporate e-portfolio as part of their job application procedures so that they stand a better chance to obtain their dream job in this competitive world. This practice is pertinent as their short of experience in job experience can be compromised in this way to highlight their ability in task accomplishments. Therefore, this study aimed to explore the perceptions of e-portfolio preparation as part of job application skills and to investigate the impact of e-portfolio practice among the final year undergraduates in the universities. The research design for this study was a quantitative method. 40 reflective journals were collected from a whole class of final year undergraduates from a public university Malaysia. Fundamentally, usefulness and challenges were two important factors that affected and impacted the final production of the e-portfolio. The participants found that e-portfolio was beneficial for them due to its usefulness in terms of five themes: learners’ ability, smoothness, attitude, motivation, and appreciation. However, there were also challenges found which were categorized under four themes: time constraint, lack of skill, accessibility, and limited software features. The study showed that despite the challenges faced in preparing the e-portfolio, the participants felt that e-portfolio has enhanced their confidence level in attending interviews in future.