Digitalis Extraction Studies. III *,†

Hopponen, Raymond E.; Gisvold, Ole

doi:10.1002/jps.3030410312

Cited by 6 publications

(8 citation statements)

References 7 publications

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“…It was hoped that cnzymatic activity could be obtained in the disintegrated leaves. The fresh leaves of Digitalis lanata were frozen and powdered as previously described (1). The frozen powder was allowed to thaw a t room temperature, suspended in distilled water, heatcd to 40".…”

Section: Isolation Of Glycosides After Enzymatic Action-mentioning

confidence: 99%

“…layered below with chloroform and above with toluene, and then incubated in an electric oven a t 40". After a given period of time the incubated mixture was heated to 65-70' (not higher) as a most suitable temperature, the mixture filtered, the marc washed with water, and the filtrate extracted with methyl isobutyl ketone as previously described (1). Several trial runs on 250-Gm.…”

Section: Isolation Of Glycosides After Enzymatic Action-mentioning

confidence: 99%

“…The mare was repeatcdly extracted with hot water (65-70") to make a 2% aqueous extract. While still warm the aqueous extract was extracted with methyl isobutyl ketone and the ketonc extract worked up as previously described (1). The crude crystalline glycoside was exhaustively extracted with petroleum ether and then with ether.…”

Section: Examinations Of Rapidly Dried Leaves Of Digitalis Lanata-clmentioning

confidence: 99%

“…They were freshly collected and examined phytochcmically as previously described here and elsewhcre (1). The recrystallized product (Fig.…”

Section: Examinations Of Rapidly Dried Leaves Of Digitalis Lanata-clmentioning

confidence: 99%

“…N PRECEDING studies it has been shown that fast-frozen and powdered leaves of Digitalis Zanata can be extracted by new techniques to yield 01-and P-acetyl digoxin as the chief crystalline glycosides (1) when this plant is grown in the Medicinal Plant Garden of the College of Pharmacy of the University of Minnesota. It appeared that the procedure employed prevented any enzymatic activity and that the native glycosides were obtained.…”

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confidence: 99%

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Digitalis Extraction Studies. IV *†

Krishnamurty

Gisvold

1952

Journal of the American Pharmaceutical Association (Scientific

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A crystalline glycosidic substance was isolated, using enzyme‐inhibiting techniques, from Digitalis lanata that was grown from seeds obtained from A. Stoll. This glycosidic material had physical and chemical properties that very closely resembled those of a glycosidic material obtained from Digitalis lanata that had been propagated over a number of years in the botanical gardens of the University of Minnesota. This glycosidic material was previously characterized as being composed chiefly of acetyl digoxin. When fresh leaves were incubated for eight, twenty‐four, and forty‐eight hours, either the same crystalline glycosidic material was obtained as previously described or no crystalline material could be isolated. Digitalis lanata leaves that had been improperly stored for seven months failed to yield any crystalline glycosidic material. By using Stoll's enzyme‐inhibiting techniques, a crystalline glycosidic material was obtained that appeared to be identical to that obtained by the new techniques described. In addition, the Stoll technique was more tedious and difficult.

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Section: Isolation Of Glycosides After Enzymatic Action-mentioning

confidence: 99%

Section: Isolation Of Glycosides After Enzymatic Action-mentioning

confidence: 99%

Section: Examinations Of Rapidly Dried Leaves Of Digitalis Lanata-clmentioning

confidence: 99%

“…They were freshly collected and examined phytochcmically as previously described here and elsewhcre (1). The recrystallized product (Fig.…”

Section: Examinations Of Rapidly Dried Leaves Of Digitalis Lanata-clmentioning

confidence: 99%

mentioning

confidence: 99%