Dihydroxynonene mercapturic acid, a urinary metabolite of 4‐hydroxynonenal, as a biomarker of lipid peroxidation

Peiro, Géraldine; Alary, J.; Cravedi, Jean‐Pierre; Rathahao, Estelle; Steghens, Jean‐Paul; Guéraud, Françoise

doi:10.1002/biof.5520240110

Cited by 30 publications

(31 citation statements)

References 25 publications

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“…An enantioselective LC-MRM/MS assay has not been developed for the analysis of DHN-MAs. However, using an achiral SID LC-MRM/MS method, it was possible to show that the urinary excretion of DHN-MA increased significantly after BrCCl 3 administration to rats compared with vehicle-treated animals (Peiro et al ., 2005). This suggested the DHN-MA could be a useful biomarker of lipid peroxidation-induced oxidative stress.…”

Section: Analysis Of Endogenous Ma Metabolitesmentioning

confidence: 99%

Analysis of endogenous glutathione‐adducts and their metabolites

Blair

2009

Biomedical Chromatography

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The ability to conduct validated analyses of glutathione (GSH)-adducts and their metabolites is critically important in order to establish whether they play a role in cellular biochemical or pathophysiological processes. The use of stable isotope dilution (SID) methodology in combination with liquid chromatography–tandem mass spectrometry (LC-MS/MS) provides the highest bioanalytical specificity possible for such analyses. Quantitative studies normally require the high sensitivity that can be obtained by the use of multiple reaction monitoring (MRM)/MS rather than the much less sensitive but more specific full scanning methodology. The method employs a parent ion corresponding to the intact molecule together with a prominent product ion that obtained by collision induced dissociation. Using SID LC-MRM/MS, analytes must have the same relative LC retention time to the heavy isotope internal standard established during the validation procedure, the correct parent ion and the correct product ion. This level of specificity cannot be attained with any other bioanalytical technique employed for biomarker analysis. This review will describe the application of SID LC-MR/MS methodology for the analysis of GSH-adducts and their metabolites. It will also discuss potential future directions for the use of this methodology for rigorous determination of their utility as disease and exposure biomarkers.

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Section: Analysis Of Endogenous Ma Metabolitesmentioning

confidence: 99%

Analysis of endogenous glutathione‐adducts and their metabolites

Blair

2009

Biomedical Chromatography

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“…Malondialdehyde was measured in serum by liquid chromatography-mass spectrometry (LC/MS) according to a method derived from an initial ultraviolet (UV) version (25) with an electrospray ionization (ESI) positive detection mode and an internal standard (dideuterated tetra methoxypropane) for correction of MDA protein binding (26). The carbonyl contents in plasma were evaluated according to Levine et al (27) and expressed as nanomoles per milligram of protein, being the protein content determined based on the method of Lowry et al (28).…”

Section: Determination Of Total Mda and Protein Carbonylsmentioning

confidence: 99%

Systemic Markers of the Redox Balance and Apolipoprotein E Polymorphism in Atherosclerosis: The Relevance for an Integrated Study

Lopes

Napoleão

Pinheiro

et al. 2006

BTER

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H u m a n a J o u r n a l s . c o mS e a r c h , R e a d a n d D o w n l o a d Featured in this issue: ABSTRACTProspective studies have demonstrated that an imbalance between oxidative damage and antioxidative protection can play a role in the development and progression of atherosclerosis. Also, genotypes with the apolipoprotein E ε4 allele have been associated with an increase risk for this pathology. Based on this knowledge, the aim of this study was to evaluate indicators of the redox balance, trace elements, and apolipoprotein E allelic profile in subjects from the Lisbon population with clinically stable atherosclerosis, at risk for atherosclerotic events, and in healthy subjects for comparison. The activities of superoxide dismutase in erythrocytes and glutathione peroxidase in whole blood, plasma total thiols, and serum ceruloplasmin were kept unchanged among the three groups. Serum α-tocopherol was increased in atherosclerotic patients. *Author to whom all correspondence and reprint requests should be addressed.lipid and protein oxidative damage, respectively, reached their highest values in risk subjects. The concentrations of potassium and calcium, in plasma and in blood cells, were slightly elevated in patients and might reflect an electrolytic imbalance. Regarding the apolipoprotein E polymorphism, atherosclerotic patients had an increased incidence of the high-risk genotypes for atherogenesis (ε3/ε4 and ε4/ε4). A multivariate model applied to the general population using most of the parameters clearly separated the three groups at study (i.e., the healthy group from the steady-state group of risk disease and from the atherosclerotic one). As shown by us, the usefulness of biochemical and complementary genetic markers is warranted for a better knowledge on atherosclerosis molecular basis.

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“…Lipid peroxidation yields end products, such as alkanes, aldehydes, and isoprostanes and a major end-product is 4-hydroxynonenal. 1,4-Dihydroxynonane mercapturic acid (DHN-MA), previously shown to be the major urinary metabolite of 4-hydroxynonenal (6), could represent a specific and noninvasive biomarker of the lipid peroxidation process (7). As heme intake, colon carcinogenesis, and high luminal peroxidation are linked, we analyzed urinary DHN-MA and 8-iso-prostaglandin-F 2 a (8-iso-PGF 2 a) excretion, the latter being a widely used lipid peroxidation biomarker (8), in three separate studies.…”

Section: Introductionmentioning

confidence: 99%

New Marker of Colon Cancer Risk Associated with Heme Intake: 1,4-Dihydroxynonane Mercapturic Acid

Pierre

Peiro

Taché

et al. 2006

Cancer Epidemiology, Biomarkers &Amp; Prevention

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Background: Red meat consumption is associated with an increased risk of colon cancer. Animal studies show that heme, found in red meat, promotes preneoplastic lesions in the colon, probably due to the oxidative properties of this compound. End products of lipid peroxidation, such as 4-hydroxynonenal metabolites or 8-iso-prostaglandin-F 2 A (8-iso-PGF 2 A), could reflect this oxidative process and could be used as biomarkers of colon cancer risk associated with heme intake. Methods: We measured urinary excretion of 8-iso-PGF 2 A and 1,4-dihydroxynonane mercapturic acid (DHN-MA), the major urinary metabolite of 4-hydroxynonenal, in three studies. In a short-term and a carcinogenesis long-term animal study, we fed rats four different diets (control, chicken, beef, and blood sausage as a high heme diet). In a randomized crossover human study, four different diets were fed (a 60 g/d red meat

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Dihydroxynonene mercapturic acid, a urinary metabolite of 4‐hydroxynonenal, as a biomarker of lipid peroxidation

Cited by 30 publications

References 25 publications

Analysis of endogenous glutathione‐adducts and their metabolites

Analysis of endogenous glutathione‐adducts and their metabolites

Systemic Markers of the Redox Balance and Apolipoprotein E Polymorphism in Atherosclerosis: The Relevance for an Integrated Study

New Marker of Colon Cancer Risk Associated with Heme Intake: 1,4-Dihydroxynonane Mercapturic Acid

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