Diketopiperazines from Rhabdastrella globostellata

Trang, Do Thi; Dung, Duong Thi; Hang, Dan Thi Thuy; Dung, Nguyen Viet; Linh, Tran My; Mai, Nguyen Chi; Yen, Pham Hai; An, Do Hoai; Nhiem, Nguyen Xuan; Van Kiem, Phan; Tai, Bui Huu

doi:10.1002/vjch.202200054

Cited by 2 publications

(2 citation statements)

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“…globostellata (Bourguet-Kondracki et al, 2000;Tasdemir et al, 2002;Clement et al, 2006;Fouad et al, 2006;Hirashima et al, 2010;Li et al, 2010;Trang et al, 2022a), and Spheciospongia spp. (Costantino et al, 2005;Whitson et al, 2008;Eltamany et al, 2015;Tawfike et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…We have previously investigated the bacterial and archaeal diversity of the 18 marine sponge species and found that particularly three species Spheciospongia sp., Rhabdastrella globostellata and Clathria reinwardti harbored diverse communities of bacteria . Furthermore, recent chemical investigations have revealed diverse secondary metabolites derived from these sponge species, including C. reinwardti (Goud et al, 2005;Tai et al, 2021;Trang et al, 2022b), R. globostellata (Bourguet-Kondracki et al, 2000;Tasdemir et al, 2002;Clement et al, 2006;Fouad et al, 2006;Hirashima et al, 2010;Trang et al, 2022a), and Spheciospongia spp. (Costantino et al, 2005;Whitson et al, 2008;Eltamany et al, 2015;Tawfike et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Diversity of prokaryotic communities associated with sponges in Vietnam and their secondary metabolite biosynthetic potential

Ton That Huu Dat

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Sponge-associated prokaryotic diversity has been studied from a wide range of marine environments across the globe. However, for certain regions, e.g. Vietnam, Thailand, Cambodia and Singapore, an overview of the sponge-associated prokaryotic communities is still pending. In this study we characterized the prokaryotic communities from 27 specimens, comprising 18 marine sponge species, sampled from the central coastal region of Vietnam. Illumina MiSeq sequencing of 16S rRNA gene fragments was used to investigate spongeassociated bacterial and archaeal diversity. Overall, 14 bacterial phyla and one archaeal phylum were identified among all 27 samples. The phylum Proteobacteria was present in all sponges and the most prevalent phylum in 15 out of 18 sponge species, albeit with pronounced differences at the class level. In contrast, Chloroflexi was the most abundant phylum in Halichondria sp., whereas Spirastrella sp. and Dactylospongia sp. were dominated by Actinobacteria. Several bacterial phyla such as Acidobacteria, Actinobacteria, Bacteroidetes, Chloroflexi, Deferribacteres, Gemmatimonadetes, and Nitrospirae were found in two-thirds of the sponge species. Moreover, the phylum Thaumarchaeota (Archaea), which is known to comprise nitrifying archaea, was highly abundant among the majority of the 18 investigated sponge species. Altogether, this study demonstrates that the diversity of prokaryotic communities associated with Vietnamese sponges is comparable to sponge-prokaryotic assemblages from well-documented regions. Furthermore, the phylogenetically divergent sponges hosted species-specific prokaryotic communities, thus demonstrating the influence of host identity on the composition and diversity of the associated communities. Therefore, this high-throughput 16S rRNA gene amplicon analysis of Vietnamese sponge-prokaryotic communities provides a foundation for future studies on sponge symbiont function and sponge-derived bioactive compounds from this region.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Diversity of prokaryotic communities associated with sponges in Vietnam and their secondary metabolite biosynthetic potential

Ton That Huu Dat

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Diversity of Bacterial Secondary Metabolite Biosynthetic Gene Clusters in Three Vietnamese Sponges

et al. 2022

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Recent reviews have reinforced sponge-associated bacteria as a valuable source of structurally diverse secondary metabolites with potent biological properties, which makes these microbial communities promising sources of new drug candidates. However, the overall diversity of secondary metabolite biosynthetic potential present in bacteria is difficult to access due to the fact that the majority of bacteria are not readily cultured in the laboratory. Thus, use of cultivation-independent approaches may allow accessing “silent” and “cryptic” secondary metabolite biosynthetic gene clusters present in bacteria that cannot yet be cultured. In the present study, we investigated the diversity of secondary metabolite biosynthetic gene clusters (BGCs) in metagenomes of bacterial communities associated with three sponge species: Clathria reinwardti, Rhabdastrella globostellata, and Spheciospongia sp. The results reveal that the three metagenomes contain a high number of predicted BGCs, ranging from 282 to 463 BGCs per metagenome. The types of BGCs were diverse and represented 12 different cluster types. Clusters predicted to encode fatty acid synthases and polyketide synthases (PKS) were the most dominant BGC types, followed by clusters encoding synthesis of terpenes and bacteriocins. Based on BGC sequence similarity analysis, 363 gene cluster families (GCFs) were identified. Interestingly, no GCFs were assigned to pathways responsible for the production of known compounds, implying that the clusters detected might be responsible for production of several novel compounds. The KS gene sequences from PKS clusters were used to predict the taxonomic origin of the clusters involved. The KS sequences were related to 12 bacterial phyla with Actinobacteria, Proteobacteria, and Firmicutes as the most predominant. At the genus level, the KSs were most related to those found in the genera Mycolicibacterium, Mycobacterium, Burkholderia, and Streptomyces. Phylogenetic analysis of KS sequences resulted in detection of two known ‘sponge-specific’ BGCs, i.e., SupA and SwfA, as well as a new ‘sponge-specific’ cluster related to fatty acid synthesis in the phylum Candidatus Poribacteria and composed only by KS sequences of the three sponge-associated bacterial communities assessed here.

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Diketopiperazines from Rhabdastrella globostellata

Cited by 2 publications

References 9 publications

Diversity of prokaryotic communities associated with sponges in Vietnam and their secondary metabolite biosynthetic potential

Diversity of prokaryotic communities associated with sponges in Vietnam and their secondary metabolite biosynthetic potential

Diversity of Bacterial Secondary Metabolite Biosynthetic Gene Clusters in Three Vietnamese Sponges

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