Dimeric-serotonin bivalent ligands induced gold nanoparticle aggregation for highly sensitive and selective serotonin biosensor

Chavan, Sachin Ganpat; Yagati, Ajay Kumar; Kim, Hyun Tae; Jin, Eunjian; Park, Sung Ryul; Patil, Dilip V.

doi:10.1016/j.bios.2021.113447

Cited by 17 publications

(10 citation statements)

References 35 publications

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“…Serotonin enzymatic oxidation reaction paralleled in vitro electrochemical oxidation at lower pH, yielding a similar product, which made it possible to investigate the probable mechanisms of the enzyme-mediated reaction shown in Figure a. During the oxidative conversion of serotonin, increases in peroxidase and H 2 O 2 concentrations, which were optimized in our recent work, caused a significant increase in the yield of dimeric serotonin . Serotonin was immediately converted to the dimeric product by the addition of 0.2 mM H 2 O 2 substrate in the presence of 24 mU/μL HRP.…”

Section: Resultsmentioning

confidence: 85%

“…a) Schematic illustration of a commercially available serotonin ELISA kit (Abcam, ab 133053) . (b) Serotonin ELISA assay using concentrations of 0, 0.5, 1.9, 7.8, 31.3, 125, and 500 ng/mL.…”

Section: Resultsmentioning

confidence: 99%

“…Our previously reported study calculated the spectroscopic characterization and kinetic equilibrium concentrations of HRP versus the H 2 O 2 system. , Briefly, the spectroscopic absorption of serotonin oxidation was recorded from 200 to 800 nm. The systematic spectroscopic observation of the initial serotonin oxidation rate at 317 nm increased with different HRP/H 2 O 2 system concentrations.…”

Section: Methodsmentioning

confidence: 99%

“…Several electrochemical methods for the determination of serotonin have been reported, including colorimetric, , spectrophotometric, and fluorometric methods as well as mass spectrometry (MS), high-performance liquid chromatography (HPLC), capillary electrophoresis, and enzyme-linked immune sorbent assays (ELISA) . However, these methods are expensive, difficult, and time consuming and often require a pre-treatment procedure, which is inconvenient for daily routine testing procedures.…”

Section: Introductionmentioning

confidence: 99%

“…We have recently performed the chemical modulation of serotonin by enzymatic peroxidation to construct a self-assembled 4,4′-dimeric serotonin complex . The capacity of the peroxidase system to oxidize indolic and phenolic compounds is well known.…”

Section: Introductionmentioning

confidence: 99%

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Conformationally Flexible Dimeric-Serotonin-Based Sensitive and Selective Electrochemical Biosensing Strategy for Serotonin Recognition

et al. 2022

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A novel electrochemical sensor was constructed based on an enzyme-mediated physiological reaction between neurotransmitter serotonin per-oxidation to reconstruct dual-molecule 4,4′-dimeric-serotonin self-assembled derivative, and the potential biomedical application of the multi-functional nano-platform was explored. Serotonin accelerated the catalytic activity to form a dual molecule at the C4 position and created phenolic radical–radical coupling intermediates in a peroxidase reaction system. Here, 4,4′ dimeric-serotonin possessed the capability to recognize intermolecular interactions between amine groups. The excellent quenching effects on top of the gold surface electrode system archive logically inexpensive and straightforward analytical demands. In biochemical sensing analysis, the serotonin dimerization concept demonstrated a robust, low-cost, and highly sensitive immunosensor, presenting the potential of quantifying serotonin at point-of-care (POC) testing. The high-specificity serotonin electrochemical sensor had a limit of detection (LOD) of 0.9 nM in phosphate buffer and 1.4 nM in human serum samples and a linear range of 10 to 400 with a sensitivity of 2.0 × 10–2 nM. The bivalent 4,4′-dimer–serotonin interaction strategy provides a promising platform for serotonin biosensing with high specificity, sensitivity, selectivity, stability, and reproducibility. The self-assembling gold surface electrochemical system presents a new analytical method for explicitly detecting tiny neurotransmitter-responsive serotonin neuromolecules.

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Section: Resultsmentioning

confidence: 85%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Conformationally Flexible Dimeric-Serotonin-Based Sensitive and Selective Electrochemical Biosensing Strategy for Serotonin Recognition

et al. 2022

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Recent advances in nanomaterials‐based optical sensors for detection of various biomarkers (inorganic species, organic and biomolecules)

Harshita

Kailasa

2022

Luminescence

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This review briefly emphasizes the different detection approaches (electrochemical sensors, chemiluminescence, surface‐enhanced Raman scattering), functional nanostructure materials (quantum dots, metal nanoparticles, metal nanoclusters, magnetic nanomaterials, metal oxide nanoparticles, polymer‐based nanomaterials, and carbonaceous nanomaterials) and detection mechanisms. Furthermore, the emphasis of this review is on the integration of functional nanomaterials with optical spectroscopic techniques for the identification of various biomarkers (nucleic acids, glucose, uric acid, oxytocin, dopamine, ascorbic acid, bilirubin, spermine, serotonin, thiocyanate, Pb2+, Cu2+, Hg2+, F−, peptides), and cancer biomarkers (mucin 1, prostate specific antigen, carcinoembryonic antigen, CA15‐3, human epidermal growth factor receptor 2, C‐reactive protein, and interleukin‐6). Analytical characteristics of nanomaterials‐based optical sensors are summarized in the tables, providing the insights of nanomaterials‐based optical sensors for biomarkers detection. Finally, the opportunities and challenges of nanomaterials‐based optical analytical approaches for the detection of various biomarkers (inorganic, organic, biomolecules, peptides and proteins) are discussed.

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Polypyrrole-palladium nanocomposite as a high-efficiency transducer for thrombin detection with liposomes as a label

et al. 2021

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Dimeric-serotonin bivalent ligands induced gold nanoparticle aggregation for highly sensitive and selective serotonin biosensor

Cited by 17 publications

References 35 publications

Conformationally Flexible Dimeric-Serotonin-Based Sensitive and Selective Electrochemical Biosensing Strategy for Serotonin Recognition

Conformationally Flexible Dimeric-Serotonin-Based Sensitive and Selective Electrochemical Biosensing Strategy for Serotonin Recognition

Recent advances in nanomaterials‐based optical sensors for detection of various biomarkers (inorganic species, organic and biomolecules)

Polypyrrole-palladium nanocomposite as a high-efficiency transducer for thrombin detection with liposomes as a label

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