Dimerization of the C-type lectin-like receptor CD93 promotes its binding to Multimerin-2 in endothelial cells

Barbera, Stefano; Raucci, Luisa; Tassone, Giusy; Tinti, Laura; Prischi, Filippo; Santucci, Annalisa; Mongiat, Maurizio; Tosi, Gian Marco; Galvagni, Federico; Dimberg, Anna; Pozzi, Cecilia; Orlandini, Maurizio

doi:10.1016/j.ijbiomac.2022.10.136

Cited by 5 publications

(2 citation statements)

References 52 publications

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“…In cluster 9 we find a mix of markers of several cell types: myeloid (Fcerg1, Ptpn18 ), erythroid (Hbb-bh1, Tal1 ), endothelial (Vwf, Pecam1, Kdr ) and megakaryocytic (Thbs1, Pf4 ), which could indicate that this group includes the hemangioblasts. Finally, in cluster 10 we can find the endothelial population (Cdh5,Pecam1,Emcn), mostly of the proliferative type (Cd93, Mmrn2, [33,34]). Supplementary Fig.…”

Section: Cell Types and Population Changes In Response To Hypoxiamentioning

confidence: 93%

An exploration of vascular cell development in mouse embryoid bodies under hypoxia through single cell RNA-seq

Acosta-Iborra,

Puente-Santamaría,

Berrouayel

et al. 2024

Preprint

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Background: Differentiation of mouse stem cells (mESC) to embryonic bodies (EBs) is a widely used approach to model early stages of development. In this study, we employed single-cell transcriptomics to examine the effect of hypoxia on the differentiation of different cell populations in EBs. Results: We differentiated EBs for 8 or 10 days, with two time frames of hypoxic or normoxic exposure: a short window of 16h for the 8-day samples, and a 48h window for the 10-day samples, totaling four experimental conditions. The EBs were composed mainly of broad mesodermal progenitors, a limited number of endodermal precursors, and smaller groups of cells that were more advanced in their differentiation pathways. These developed groups included hemoendothelial progenitors, endothelium, cardiomyocytes, and monocyte and erythrocyte precursors. Both O2 and time of differentiation significantly influenced the distribution of cell types across conditions. Our findings revealed that hypoxia induces a pervasive cell cycle arrest signature across all identified cell populations within the EB. Despite the cell cycle arrest, vascular smooth muscle (vSMCs), endothelial, and cardiac cell populations exhibit expansion under hypoxic conditions, while normoxia favours an increase in the monocyte population. Differential proliferation of hemoendothelial progenitors does not appear to be the primary driver of endothelial cells expansion in hypoxia, as their distribution remained similar across treatments and depleted over time. We observed delays in the terminal differentiation pathway of vSMCs precursors in hypoxic conditions, which may contribute to their expansion and migration by switching to a synthetic phenotype. Additionally, oxygen concentration influenced the developmental path of hemangioblasts towards either an endothelial, or myeloid lineage. In the EBs, this was coupled with a shift in presomitic mesoderm differentiation towards endotome or musculoskeletal precursors, which may provide an additional source of progenitors with endothelial potential during oxygen deprivation. Conclusion: Our study provides novel insights into the cellular responses to hypoxia in the context of early embryonic development, thus unveiling potential mechanisms underlying blood vessel growth.

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Section: Cell Types and Population Changes In Response To Hypoxiamentioning

confidence: 93%

An exploration of vascular cell development in mouse embryoid bodies under hypoxia through single cell RNA-seq

Acosta-Iborra,

Puente-Santamaría,

Berrouayel

et al. 2024

Preprint

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“…For instance, it was recently shown that collectin-11 plays an important role in melanoma cell proliferation and tumor growth in mice model ( 20 ). Consequently, it was just reported that in endothelial cells dimerization of the C-type lectin-like receptor CD93 promotes its binding to multimerin-2 ( 21 ). These observations provide insight into the future design of new drugs able to hamper CD93 function in neovascular pathologies.…”

Section: Methodsmentioning

confidence: 99%

Anti(angiogenic) food components: can be a major source of bias in the investigation of angiogenesis inhibitors

Rastmanesh,

Bowirrat,

Gupta

et al. 2023

Ann Transl Med

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Background Natural and diet-derived angiogenesis inhibitors/promotors are widely found in diets. These compounds can in several ways impact the results of oncological research of angiogenesis inhibitors. Methods We very briefly overview some of the most important examples to show how these compounds can create a bias in current research of cancer. Implications of this expert opinion cover similar angiogenesis-related diseases. Results Significant intra-individual differences in terms of dietary intake and differential effect of food processing techniques result in differential bioactivity and bioavailability of these compounds. There are only a handful of validated dietary questionnaire to quantify natural angiogenesis inhibitors/promotors. A corollary consequence is that participants in non-randomized clinical trials will have different baseline levels of serum/plasma/tissue/organ diet-derived angiogenesis inhibitors/promotors. This will lead to creation of clinical uncertainty and a hidden bias and consequently creation of translational efficiency bias, sampling efficiency, and waste of resources. We call for developing and validating a semi-quantitative food frequency questionnaire (FFQ) to gather data on these agents, specifically designed for oncological research because there is a clear gap in the literature of oncology. Conclusions This might facilitate the discovery of better prognostic, diagnostic, preventive measures, and therapeutic agents for the management of different cancers. Implications of this paper cover similar settings like ophthalmologic research.

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Structural insight into CD93 recognition by IGFBP7

Xu,

Sun,

Zhu

et al. 2024

Structure

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Dimerization of the C-type lectin-like receptor CD93 promotes its binding to Multimerin-2 in endothelial cells

Cited by 5 publications

References 52 publications

An exploration of vascular cell development in mouse embryoid bodies under hypoxia through single cell RNA-seq

An exploration of vascular cell development in mouse embryoid bodies under hypoxia through single cell RNA-seq

Anti(angiogenic) food components: can be a major source of bias in the investigation of angiogenesis inhibitors

Structural insight into CD93 recognition by IGFBP7

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