Dimethylthiourea, a hydrogen peroxide trap, partially prevents stress effects and ascorbate peroxidase increase in spermidine-treated maize roots

Zacchini, Massimo; Agazio, M. De

doi:10.1046/j.1365-3040.2001.00670.x

Cited by 1 publication

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References 28 publications

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“…As shown in Supplemental Table S1, 1 mM Spd slightly affected root growth while 10 mM Spd was the lowest concentration at which almost complete inhibition of root total growth (94% 6 9%) was observed when compared to untreated controls, and hence this concentration was hereafter used in combined hydroponics culture treatments, such as Spd and G3, Spd and DMTU, as well as Spd and L-ascorbic acid. Put, Spm, and ABAL were supplied at a concentration equimolar to 10 mM Spd, while G3 and DMTU were used at the working concentration of 10 mM and 5 mM, respectively, as previously reported (Zacchini and De Agazio, 2001;Angelini et al, 2008). Treatment with 10 mM or 1 mM Spd resulted in a decrease in the average length of cortical parenchymal cells (Table I) without affecting PAO enzymatic activity, protein abundance (Supplemental Fig.…”

Section: Cell-and Tissue-specific Expression Of Pao In Maize Primary mentioning

confidence: 99%

Perturbation of Polyamine Catabolism Can Strongly Affect Root Development and Xylem Differentiation

et al. 2011

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Spermidine (Spd) treatment inhibited root cell elongation, promoted deposition of phenolics in cell walls of rhizodermis, xylem elements, and vascular parenchyma, and resulted in a higher number of cells resting in G 1 and G 2 phases in the maize (Zea mays) primary root apex. Furthermore, Spd treatment induced nuclear condensation and DNA fragmentation as well as precocious differentiation and cell death in both early metaxylem and late metaxylem precursors. Treatment with either N-prenylagmatine, a selective inhibitor of polyamine oxidase (PAO) enzyme activity, or N,N 1 -dimethylthiourea, a hydrogen peroxide (H 2 O 2 ) scavenger, reverted Spd-induced autofluorescence intensification, DNA fragmentation, inhibition of root cell elongation, as well as reduction of percentage of nuclei in S phase. Transmission electron microscopy showed that N-prenylagmatine inhibited the differentiation of the secondary wall of early and late metaxylem elements, and xylem parenchymal cells. Moreover, although root growth and xylem differentiation in antisense PAO tobacco (Nicotiana tabacum) plants were unaltered, overexpression of maize PAO (SZmPAO) as well as down-regulation of the gene encoding S-adenosyl-L-methionine decarboxylase via RNAi in tobacco plants promoted vascular cell differentiation and induced programmed cell death in root cap cells. Furthermore, following Spd treatment in maize and ZmPAO overexpression in tobacco, the in vivo H 2 O 2 production was enhanced in xylem tissues. Overall, our results suggest that, after Spd supply or PAO overexpression, H 2 O 2 derived from polyamine catabolism behaves as a signal for secondary wall deposition and for induction of developmental programmed cell death.

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Section: Cell-and Tissue-specific Expression Of Pao In Maize Primary mentioning

confidence: 99%

Perturbation of Polyamine Catabolism Can Strongly Affect Root Development and Xylem Differentiation

et al. 2011

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show abstract

Dimethylthiourea, a hydrogen peroxide trap, partially prevents stress effects and ascorbate peroxidase increase in spermidine-treated maize roots

Cited by 1 publication

References 28 publications

Perturbation of Polyamine Catabolism Can Strongly Affect Root Development and Xylem Differentiation

Perturbation of Polyamine Catabolism Can Strongly Affect Root Development and Xylem Differentiation

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