Diminished interleukin 2 production and receptor generation characterize the acquired immunodeficiency syndrome

Ebert, Ellen C.; Stoll, D; Cassens, Brett J.; Lipshutz, William; Hauptman, Stephen P.

doi:10.1016/0090-1229(85)90099-6

Cited by 17 publications

(2 citation statements)

References 33 publications

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“…The CD4+ lymphocytes produce the growth factors required for cellular proliferation, primarily IG2. Consequently, lymphocytes from AIDS tients show a decreased proliferative capability mainly due to a reduced production and utilization of IL2, and to a reduced expression of IL2R (11,12).…”

Section: Introductionmentioning

confidence: 99%

Effects of Pulsed Electromagnetic Fields on the Proliferation of Lymphocytes from Aids Patients, HIV-Seropositive Subjects, and Seronegative Drug Users

Cossarizza

Borghi

Bersani

et al. 1989

Journal of Bioelectricity

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The effect of the exposure of mitogen-stimulated lymphocytes from subjects infected by human immunodeficiency virus to extremely low frequency pulsed electromagnetic fields (PEMFs) was studied, by evaluating the incorporation of tritiated thymidine, the expression of I L 2 receptor, and the amount of activated T lymphocytes. Four groups of subjects were considered patients with acquired immunodeficiency syndrome (AIDS), asymptomatic seropositive subjects, seronegative drug users, and young healthy controls. PEMFs increased cell proliferation only in the group of healthy controls, as measured at the 72nd hour of culture, but a n increase in the number of activated T lymphocytes was observed by cytofluorimetric analysis after 18 hrs of PEMF exposure in cultures from AIDS patients.

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Section: Introductionmentioning

confidence: 99%

Effects of Pulsed Electromagnetic Fields on the Proliferation of Lymphocytes from Aids Patients, HIV-Seropositive Subjects, and Seronegative Drug Users

Cossarizza

Borghi

Bersani

et al. 1989

Journal of Bioelectricity

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“…Early reports indicated that IL-2 was synthesized by both CD4+ demonstrated that exogenous IL-2 can restore in vitro T cell and CD8+ T cell subpopulations [3][4][5], but CD4+ T helper/ immunity. Exogenous IL-2 can augment proliferative responses inducer cells appear to be the major source of IL-2 after antigento recall antigens [15], alloantigens and mitogens [2,16,17], purified native HIV [18,19], and restore natural killer (NK) and mediated activation [6][7][8], and CD4+ T helper/inducer cell I X.I,...r--numbersãr sinfcnl reue duin th I-nue virus-specific cytotoxic T lymphocyte (CTL) activity [20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Heterogeneous effects of exogenous IL-2 on HIV-specific cell-mediated immunity (CMI)

Bell

Cooper

Kemp

et al. 1992

Clinical and Experimental Immunology

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SUMMARYA characteristic feature associated with HIV-l infection of the human host is a chronic decline in circulating CD4+ T helper/inducer cell numbers. Impaired cell-mediated immune functions usually occur in parallel with the decline in CD4+ T cells. Activated CD4+ T helper cells are a major source of endogenous IL-2 which is required for the immunoregulation of both antigen-specific B cells and CD8+ T cells. HIV-specific T cell proliferative responses are said to be weak and inconsistent, even during the asymptomatic phase of disease. We thus wished to determine how exogenous IL-2 affected HIV-specific T cell proliferation at different stages of the disease. Our cohort of 81 included both asymptomatic and symptomatic HIV-infected patients as well as uninfected normal donors. Proliferative responses of peripheral blood mononuclear cells (PBMC) that were elicited during culture with an immunodominant gp41-derived synthetic peptide, gp4l{8}, and which were known to be CD8+ cell-associated in asymptomatics only, were used to analyse the effects of exogenous IL-2. IL-2 had three main effects on HIV-specific proliferation, namely (i) an additive effect, (ii) a synergistic effect, and (iii) an induced effect. More specifically, low dose exogenous IL-2 frequently augmented lymphoproliferation in both asymptomatic and symptomatic gp4l{8} responders. In most symptomatics, however, who were predominantly gp4l{8} non-responders, exogenous IL-2 induced lymphoproliferation. Flow cytometric analyses using dual immunofluorescence were used to analyse the T cell subset distribution of proliferating PBMC cultures. During culture with gp4l{8}, both CD4+ and CD8+ T cell numbers increased. However, after the addition of exogenous IL-2 to gp4l{8}-containing cultures, CD8+ cell-associated lymphoproliferative responses were preferentially augmented. These results suggest that in symptomatics there is an inadequate supply of endogenous IL-2 to help maintain the strong and effective CD8 + cell-associated anti-viral immunity, and an exogenous supply of IL-2 may be required.

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Effect of Mitogens on the Cell Cycle Progression and the Quantification of T-Lymphocyte Surface Markers in Acquired Immune Deficiency Syndrome

Hornicek

Malinin

Thornthwaite

et al. 1987

Journal of Leukocyte Biology

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The cell cycle progression and viability of stimulated and intact lymphocytes from 20 subjects with acquired immune deficiency syndrome (AIDS) was determined by flow cytometry. As compared to controls, 62% less AIDS lymphocytes, cultured for 72 hr in the presence of lectins (Con-A, PHA, PWM), had entered the proliferative phases of the cell cycle, while the respective value for periodic-acid (H5IO6)-stimulated cells was 34%. The helper-suppressor ratios and natural kill cell percentages of the unstimulated and PHA-activated AIDS lymphocytes increased approximately 3-fold after 72 hr in culture. The natural killer (NK) cell fraction of the PHA-stimulated and unstimulated AIDS cultures comprised approximately 20% as compared to 10% in controls. However, no changes in the percentages of T-lymphocytes were detected in the AIDS cell cultures. Throughout the culture period, viability of the unstimulated AIDS lymphocytes exceeded 90%, whereas in stimulated cultures it fluctuated within the 65-90% range. It is concluded that the liability of AIDS lymphocytes to mitogens is probably a direct consequence of the human immunodeficiency virus (HIV) infection.

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Diminished interleukin 2 production and receptor generation characterize the acquired immunodeficiency syndrome

Cited by 17 publications

References 33 publications

Effects of Pulsed Electromagnetic Fields on the Proliferation of Lymphocytes from Aids Patients, HIV-Seropositive Subjects, and Seronegative Drug Users

Effects of Pulsed Electromagnetic Fields on the Proliferation of Lymphocytes from Aids Patients, HIV-Seropositive Subjects, and Seronegative Drug Users

Heterogeneous effects of exogenous IL-2 on HIV-specific cell-mediated immunity (CMI)

Effect of Mitogens on the Cell Cycle Progression and the Quantification of T-Lymphocyte Surface Markers in Acquired Immune Deficiency Syndrome

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