Diminishing microbiome richness and distinction in the lower respiratory tract of lung cancer patients: A multiple comparative study design with independent validation

Jin, Jing; Gan, Yuncui; Liu, Huayong; Wang, Zirong; Yuan, Jianying; Deng, Taibing; Zhou, Yongzhao; Zhu, Yingying; Zhu, Huilian; Sai, Yang; Shen, Wei; Xie, Dan; Wu, Honglong; Liú, Dan; Liu, Weimin

doi:10.1016/j.lungcan.2019.08.022

Cited by 68 publications

(56 citation statements)

References 27 publications

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“…14 The relative abundance of Bradyrhizobium japonicum was increased in lung cancer patients with early-stage tumors compared with those in patients with advanced-stage tumors (I & II vs III & IV). 15 The analysis of V1-V2 region of 16S rRNA showed that Methylobacteriaceae was abundant in malignant disease compared to benign disease in breast cancer patients. 16 An intra-tumoral microbiome signature including Streptomyces and Bacillus clausii is highly predictive of long-term survivorship.…”

Section: Discussionmentioning

confidence: 99%

<p>Analyses of Potential Driver and Passenger Bacteria in Human Colorectal Cancer</p>

Wang¹,

Zhang²,

Hou³

et al. 2020

CMAR

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Introduction Besides genetic and epigenetic alterations that lead to carcinogenesis and development of colorectal cancer (CRC), intestinal microbiomes are recently recognized to play a critical role in CRC progression. The abundant species associated with human CRC have been proposed for their roles in promoting tumorigenesis. However, a recent “driver-passenger” model suggests that these CRC-associated species with high relative abundances may be passenger bacteria that take advantage of the tumor environment instead of initiating CRC, whereas the driver species that initiate CRC have been replaced by passenger bacteria due to the alteration of the intestinal niche. Methods Here, to reveal potential driver and passenger bacteria during CRC progression, we compare the gut mucosal microbiomes of 75 triplet-paired CRC samples collected from on-tumor site, adjacent-tumor site, and off-tumor site, and 26 healthy controls. Results Our analyses revealed potential driver bacteria in four genera and two families, and potential passenger bacteria in 14 genera or families. Bacillus, Bradyrhizobium, Methylobacterium, Streptomyces, Intrasporangiaceae and Sinobacteraceae were predicted to be potential driver bacteria. Moreover, 14 potential passenger bacteria were identified and divided into five groups. Group I passenger bacteria contain Fusobacterium, Campylobacter, Streptococcus, Schwartzia , and Parvimonas . Group II passenger bacteria contain Dethiosulfatibacter, Selenomonas, Peptostreptococus, Leptotrichia . Group III passenger bacteria contain Granulicatella . Group IV passenger bacteria contain Shewanella, Mogibacterium , and Eikenella . Group V passenger bacteria contain Anaerococus . Co-occurrence network analysis reveals a low correlation relationship between driver and passenger bacteria in CRC patients compared with healthy controls. Discussion These driver and passenger species may serve as bio-marker species for screening cohorts with high risk to initiate CRC or patients with CRC, respectively. Further functional studies will help understand the roles of driver and passenger bacteria in CRC initiation and development.

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Section: Discussionmentioning

confidence: 99%

<p>Analyses of Potential Driver and Passenger Bacteria in Human Colorectal Cancer</p>

Wang¹,

Zhang²,

Hou³

et al. 2020

CMAR

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“…At the genus level, Streptococcus was more abundant in the (Liu H. X. et al, 2018). Yu et al discovered that the alpha diversity in lung tumor tissues was lower than normal tissues, which was the same as the discovery by Jin J et al (Yu et al, 2016;Jin et al, 2019). The phylum Proteobacteria (predominantly genera Acinetobacter and Acidovorax) showed a higher abundance and phylum Firmicutes (genus Streptococcus) and Bacteroides (genus Prevotella) exhibited a lower abundance in patients with lung cancer than emphysema .…”

Section: Lung Cancermentioning

confidence: 71%

Microbes in Tumoral In Situ Tissues and in Tumorigenesis

Xue

Han

et al. 2020

Front. Cell. Infect. Microbiol.

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Cancerous tumors are severe diseases affecting human health that have a complicated etiology and pathogenesis. Microbes have been considered to be related to the development and progression of numerous tumors through various pathogenic mechanisms in recent studies. Bacteria, which have so far remained the most studied microbes worldwide, have four major possible special pathogenic mechanisms (modulation of inflammation, immunity, DNA damage, and metabolism) that are related to carcinogenesis. This review aims to macroscopically summarize and verify the relationships between microbes and tumoral in situ tissues from cancers of four major different systems (urinary, respiratory, digestive, and reproductive); the abovementioned four microbial pathogenic mechanisms, as well as some synergistic pathogenic mechanisms, are also discussed. Once the etiologic role of microbes and their precise pathogenic mechanisms in carcinogenesis are known, the early prevention, diagnosis, and treatment of cancers would progress significantly.

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“…Growing pieces of evidence suggested that the development of cancer is affected by human commensal microbiota through inflammation, immunity, metabolism pathways [29]. Recently, various studies identified the alteration of airway microbiota among NSCLC patients [5,7,9,11,[30][31][32][33][34]. The interplay between microbiota and lung cancer is complex.…”

Section: Discussionmentioning

confidence: 99%

The Airway Microbiota of Non-small-cell Lung Cancer Patients and its Relationship to Tumor Stage and EGFR Gene Mutation

Huang

et al. 2021

Preprint

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Background: Accumulating studies have suggested the airway microbiota of lung cancer was significantly different from healthy controls. However, little was known about the relationship between airway microbiota and important clinical parameters of lung cancer. In this study, we aimed to explore the association between sputum microbiota and lung cancer stage, lymph node metastasis, intrathoracic metastasis, and Epidermal growth factor receptor (EGFR) gene mutation. Methods: The microbiota of sputum samples from 85 newly diagnosed NSCLC patients were sequenced via 16S rRNA sequencing with V3-V4 region. Sequencing reads were filtered using QIIME2 and clustered against UPARSE. Results: The α diversity and β diversity was significantly different between patients in stage I to II (early stage, ES) and patients in stage III to IV (advanced stage, AS). Lefse identified that genera Granulicatella and Actinobacillus were significantly enriched in ES, and genus Actinomyces were significantly enriched in AS. PICRUSt2 identified NAD salvage pathway was significantly enriched in AS, which was positively associated with Granulicatella. Patients with intrathoracic metastasis were associated with increased genus Peptostreptococcus and incomplete reductive TCA cycle. Enrichment of TCA cycle was associated with increased Peptostreptococcus. Genera Parvimonas, Pseudomona and L-valine biosynthesis were positively associated with lymph node metastasis. L-valine biosynthesis was related with increased Pseudomona. Finally, genus Parvimonas were significantly upregulated in adenocarcinoma patients with EGFR mutation. Conclusion: In conclusion, the taxonomy structure differed between different lung cancer stage. The tumor stage, intrathoracic 1 metastasis, lymph node metastasis, and EGFR mutation were associated with alteration of specific airway genera and metabolic function of sputum microbiota.

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Diminishing microbiome richness and distinction in the lower respiratory tract of lung cancer patients: A multiple comparative study design with independent validation

Cited by 68 publications

References 27 publications

<p>Analyses of Potential Driver and Passenger Bacteria in Human Colorectal Cancer</p>

<p>Analyses of Potential Driver and Passenger Bacteria in Human Colorectal Cancer</p>

Microbes in Tumoral In Situ Tissues and in Tumorigenesis

The Airway Microbiota of Non-small-cell Lung Cancer Patients and its Relationship to Tumor Stage and EGFR Gene Mutation

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