Abstract. DINOSTRAT version 2.1-GTS2020 is now available (https://doi.org/10.5281/zenodo.10506652, Bijl et al., 2024b). This version updates DINOSTRAT to the Geologic Time Scale 2020, and new publications are added into the database. The resulting database now contains over 9450 entries from 209 sites. This update has not led to major and profound changes in the conclusions made previously. DINOSTRAT allows full presentation of the first and last stratigraphic occurrences of dinoflagellate cyst subfamilies and families, as well as the evolutionary turnover throughout geologic history, as a reliable representation of dinoflagellate evolution. Although the picture of dinoflagellate evolution from DINOSTRAT is broadly consistent with that in previous publications, with DINOSTRAT the underlying data are openly available, reproducible and up to date. This release of DINOSTRAT allows calibration of stratigraphic records to the Geologic Time Scale 2020 using dinoflagellate cysts as a biostratigraphic tool.