Dinuclear Ruthenium(II) Complexes as Two‐Photon, Time‐Resolved Emission Microscopy Probes for Cellular DNA

Baggaley, Elizabeth; Gill, Martin R.; Green, Nicola; Turton, David R.; Sazanovich, Igor V.; Botchway, Stanley W.; Smythe, Carl; Haycock, John W.; Weinstein, Julia A.; Thomas, Jim A.

doi:10.1002/anie.201309427

Cited by 170 publications

(95 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We have previously reported dinuclear ruthenium(II) complexes that function as DNA-imaging agents. [35][36][37][38] These com-plexes employ the ditopic tetrapyrido[3,2-a:2',3'-c:3'',2''-h:2''',3'''-j]phenazine (tpphz) as the bridging ligand. [39] In these studies, we found that cellular uptake was highly dependent on the nature of the ancillary ligands: although complex 1 is an excellent nuclear stain for live cell, its Ru II (bpy) 2 [40] and Ru II -V IV [41] mixed-metal systems, these are first reported Ir III -Ru II complexes using tpphz as a bridging ligand.…”

Section: Introductionmentioning

confidence: 99%

Tuning the Cellular Uptake Properties of Luminescent Heterobimetallic Iridium(III)–Ruthenium(II) DNA Imaging Probes

Wragg

Gill

Turton

et al. 2014

Chemistry A European J

Self Cite

View full text Add to dashboard Cite

The synthesis of two new luminescent dinuclear Ir(III)-Ru(II) complexes containing tetrapyrido[3,2-a:2',3'-c:3'',2''-h:2''',3'''-j]phenazine (tpphz) as the bridging ligand is reported. Unlike many other complexes incorporating cyclometalated Ir(III) moieties, these complexes display good water solubility, allowing the first cell-based study on Ir(III)-Ru(II) bioprobes to be carried out. Photophysical studies indicate that emission from each complex is from a Ru(II) excited state and both complexes display significant in vitro DNA-binding affinities. Cellular studies show that each complex is rapidly internalised by HeLa cells, in which they function as luminescent nuclear DNA-imaging agents for confocal microscopy. Furthermore, the uptake and nuclear targeting properties of the complex incorporating cyclometalating 2-(4-fluorophenyl)pyridine ligands around its Ir(III) centre is enhanced in comparison to the non-fluorinated analogue, indicating that fluorination may provide a route to promote cell uptake of transition-metal bioprobes.

show abstract

Section: Introductionmentioning

confidence: 99%

Tuning the Cellular Uptake Properties of Luminescent Heterobimetallic Iridium(III)–Ruthenium(II) DNA Imaging Probes

Wragg

Gill

Turton

et al. 2014

Chemistry A European J

Self Cite

View full text Add to dashboard Cite

show abstract

“…15 A recent report of DNA staining with dinuclear ruthenium complexes confirms the potential of time-resolved imaging. 16 Likewise, pH-responsive iridium complexes are effective for time-resolved imaging of the cytoplasm. 17 …”

mentioning

confidence: 99%

Luminogenic iridium azide complexes

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Because of the differences in specific responsive groups and PET process, the P-GSH, P-HP and P-HA have different sensitivity for detection of GSH, H 2 O 2 and HOCl. Metal complex-based probes have been reported to be particularly suitable for FLIM imaging because their fluorescence lifetime (typically more than 50 ns) is much longer than those of tissue (2–3 ns) and most organic dyes (1–5 ns)23. So we further measured the fluorescence lifetime of P-GSH in PBS.…”

Section: Resultsmentioning

confidence: 99%

Two-photon dual imaging platform for in vivo monitoring cellular oxidative stress in liver injury

Wang

Zhang

Bridle

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Oxidative stress reflects an imbalance between reactive oxygen species (ROS) and antioxidants, which has been reported as an early unifying event in the development and progression of various diseases and as a direct and mechanistic indicator of treatment response. However, highly reactive and short-lived nature of ROS and antioxidant limited conventional detection agents, which are influenced by many interfering factors. Here, we present a two-photon sensing platform for in vivo dual imaging of oxidative stress at the single cell-level resolution. This sensing platform consists of three probes, which combine the turn-on fluorescent transition-metal complex with different specific responsive groups for glutathione (GSH), hydrogen peroxide (H2O2) and hypochlorous acid (HOCl). By combining fluorescence intensity imaging and fluorescence lifetime imaging, these probes totally remove any possibility of crosstalk from in vivo environmental or instrumental factors, and enable accurate localization and measurement of the changes in ROS and GSH within the liver. This precedes changes in conventional biochemical and histological assessments in two distinct experimental murine models of liver injury. The ability to monitor real-time cellular oxidative stress with dual-modality imaging has significant implications for high-accurate, spatially configured and quantitative assessment of metabolic status and drug response.

show abstract

Dinuclear Ruthenium(II) Complexes as Two‐Photon, Time‐Resolved Emission Microscopy Probes for Cellular DNA

Cited by 170 publications

References 32 publications

Tuning the Cellular Uptake Properties of Luminescent Heterobimetallic Iridium(III)–Ruthenium(II) DNA Imaging Probes

Tuning the Cellular Uptake Properties of Luminescent Heterobimetallic Iridium(III)–Ruthenium(II) DNA Imaging Probes

Luminogenic iridium azide complexes

Two-photon dual imaging platform for in vivo monitoring cellular oxidative stress in liver injury

Contact Info

Product

Resources

About