Diodos emissores de luz e concentrações de BAP na multiplicação in vitro de morangueiro

Rocha, P. S. G. da; Oliveira, R. P. de; Scivittaro, W. B.; Santos, Ulisses Lyra dos

doi:10.1590/s0103-84782010000900011

Cited by 26 publications

(31 citation statements)

References 14 publications

(11 reference statements)

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“…Average plantlet length showed intermediate values under Growlux fluorescent lamps and green LEDs, and the least values under white fluorescent lamps and blue LEDs (Table 1). Kim et al (2004) and Rocha et al (2010) had already observed longer plantlets of chrysanthemum and strawberry when grown under red LEDs than under other sources of light. With other potato cultivars, Wilson et al (1993) obtained longer plantlets under red LEDs, compared to plantlets grown under white fluorescent lamps or even under blue LEDs.…”

Section: Resultsmentioning

confidence: 99%

“…LEDs provide radiant energy for better potato explant and plantlet development in laboratory rooms, and also, they show the advantage of longer useful life that may reach 100,000 hours, meanwhile fluorescent lamps present an average useful life of 8,000 hours, and incandescent lamps of 1,000 hours (ROCHA et al, 2010). According to these authors, another advantage is the energy saving, since LEDs present high energetic efficiency (50%) compared to fluorescent lamps (20%) and incandescent lamps (5%).…”

Section: Resultsmentioning

confidence: 99%

“…Recently developed light emission diodes (LEDs) have been pointed out by authors as potential sources of light for in-vitro environments cultivation. LEDs are characterized by specific wavelengths, small mass and volume, long useful life, low heating and highly efficient light generation process (60%), and do not contain mercury or other hazard element to the environment (YEH;CHUNG, 2009 ( NHUT et al, 2002), strawberry (ROCHA et al, 2010), cotton (LI et al, 2010), among others. This work aimed to optimize the in-vitro propagation of three potato cultivars, using new light sources in the culture environment.…”

Section: Introductionmentioning

confidence: 99%

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New light sources for in-vitro potato micropropagation

Rocha¹,

Oliveira²,

Scivittaro³

2015

Biosci. J.

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This research work objective was to optimize the micropropagation of potato cultivars through the use of new light sources in the growth rooms. Treatments consisted of three potato cultivars (Asterix, Catucha and Macaca), and five light sources (blue, green and red LEDs; Growlux and white fluorescent lamps). The explants consisted of nodal segments containing one bud, isolated from plantlets grown in vitro. The experimental design was completely randomized arranged in a 3x5 factorial, with eight replications. Each experimental unity consisted of a flask with five explants. Three 28-day consecutive subcultures were carried out in MS semi-solid medium, in growth-room under controlled conditions (temperature = 25+2 ºC; photoperiod = 16 hours; light intensity = 20 µmol m -2 s -1 ). At the end of each subculture, the bud number per plantlet, plantlet length and internode length were evaluated. After the third subculture, the concentrations of carotenoids and a-and b-chlorophylls were also determined. Different micropropagation efficiencies were found among potato cultivars grown in vitro conditions: 'Macaca' was the most and 'Catucha' the least responsive cultivar. The growth room light sources differently affected the potato plantlet development: red and green LEDs were the most and least recommended for plantlet development, based on the results of bud number per plantlet, plantlet length, and leaflet concentrations of a-and b-chlorophylls and carotenoids.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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New light sources for in-vitro potato micropropagation

Rocha¹,

Oliveira²,

Scivittaro³

2015

Biosci. J.

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“…Greater chlorophyll a and b content was found in plants grown in the simplified medium for light sources L4 and L7. Rocha et al (2010) and Nhut et al (2003) observed greater chlorophyll content in strawberry shoots under red and red combined with blue LEDs, respectively. Light source L4 shows a tendency to red, corroborating with these authors' findings.…”

Section: Resultsmentioning

confidence: 93%

“…Although currently fluorescent lights are the most used (POUDEL et al, 2008), studies have been carried out to investigate the quality of the light emitted by LEDs (Light Emitting Diode) in promoting growth and morphogenesis in seedlings propagated in vitro (HEO et al, 2006;MENGXI et al, 2011;ROCHA et al, 2010). In addition, they found signs that the quality of light may affect the plants acclimatization process (NHUT et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Light sources and culture media in the in vitro growth of the Brazilian orchid Microlaelia lundii

Favetta

Colombo

Júnior

et al. 2017

SCA

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Light sources and culture media in the in vitro growth of the Brazilian orchid Microlaelia lundii AbstractThe Brazilian micro-orchid Microlaelia lundii enchants by its beautiful blooms in winter, but its cultivation is still a challenge, requiring specific care. The objective of this work was to evaluate the influence of light sources and culture media on in vitro growth and ex vitro survival of this micro-orchid. M. lundii seedlings were transferred to flasks containing the culture medium ½ MS and Simplified Medium, consisting of 5 mL L -1 of Biofert Plus® NPK fertilizer (09-08-09) and 60 g L -1 of 'Nanica' banana pulp. These flasks were placed in a growth chamber composed of seven environments, characterized by types of lighting: L1) LEDtube 4000K; L2) LEDtube 6500K; L3) L1 + L2; L4) L1 + L1; L5) red LED; L6) blue LED; L7) fluorescent lamp (control). After 200 days of cultivation, biometric growth parameters and leaves chlorophyll content were evaluated together with the percentage of seedling survival when acclimatized. At the end of the experiment, an analysis of individual variances and a combined analysis of the environments were carried out. The simplified medium led to higher seedlings, especially for light source L6. Light sources L1, L2, L3 and L4 promoted an increase in the seedlings root system in the simplified medium. As for the chlorophyll content, L4 and L7 showed higher contents of chlorophyll a and b. The simplified medium led practically to 100% of seedling survival, regardless of the light source used. Results shown that the simplified medium under light sources L1, L2, L3 and L4 is recommended for the in vitro propagation of M. lundii. Key words: LED. Nutritious medium. Orchidaceae. Quality of light. ResumoA micro-orquídea brasileira Microlaelia lundii encanta pelas belas floradas no inverno, no entanto o seu cultivo ainda é um desafio, requerendo cuidados específicos. Assim, objetivou-se avaliar a influência de fontes de luz e meios de cultura no crescimento in vitro e na sobrevivência ex vitro dessa microorquídea. Plântulas de M. lundii foram transferidas para frascos contendo os meios de cultura: ½ MS e Meio Simplificado, composto por 5 mL L -1 do fertilizante Biofert Plus ® NPK (08-09-09) e 60 g L -1de polpa de banana 'Nanica'. Estes frascos foram acondicionados em câmara de cultivo composta por sete ambientes, caracterizados pelo tipo de iluminação: L1) LEDtube 4000K; L2) LEDtube 6500K; L3) L1 + L2; L4) L1 + L1; L5) LED vermelho; L6) LED azul; L7) Lâmpada fluorescente (controle o teor de clorofila nas folhas. Também, avaliou-se a porcentagem de sobrevivência das plantas, quando aclimatizadas. O meio simplificado propiciou plântulas mais altas, com destaque para a fonte de luz L6. As fontes de luz L1, L2, L3 e L4 no meio simplificado proporcionaram aumento do sistema radicular das plântulas. Quanto ao teor de clorofila, L4 e L7 apresentaram maiores teores de clorofila a e b. O meio simplificado propiciou, praticamente, 100% de sobrevivência das plantas, independente da fonte de luz util...

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Using LED lighting in somatic embryogenesis and micropropagation of an elite sugarcane variety and its effect on redox metabolism during acclimatization

Ferreira

Silva

Ulisses

et al. 2016

Plant Cell Tiss Organ Cult

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Diodos emissores de luz e concentrações de BAP na multiplicação in vitro de morangueiro

Abstract: RESUMO Realizou-se um estudo para avaliar o efeito de fontes de luz na micropropagação de morangueiro, com níveis crescentes de BAP no meio de cultivo. Para tanto, inocularamse gemas de brotações da cultivar 'Sabrosa' em meio MS com BAP (0; 0,3; 0,6; 0,9; e 1,

Cited by 26 publications

References 14 publications

New light sources for in-vitro potato micropropagation

New light sources for in-vitro potato micropropagation

Light sources and culture media in the in vitro growth of the Brazilian orchid Microlaelia lundii

Using LED lighting in somatic embryogenesis and micropropagation of an elite sugarcane variety and its effect on redox metabolism during acclimatization

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