Dioscin and methylprotodioscin isolated from the root of Asparagus cochinchinensis suppressed the gene expression and production of airway MUC5AC mucin induced by phorbol ester and growth factor

Kim

et al. 2019

Phytotherapy Research

Self Cite

We investigated whether obtusin, obtusifolin, and cassiaside isolated from the seeds of Cassia obtusifolia inhibit the gene expression and production of airway mucin 5AC (MUC5AC). Confluent NCI-H292 cells were pretreated with obtusin, obtusifolin, or cassiaside for 30 min and then stimulated with epidermal growth factor (EGF), phorbol 12-myristate 13-acetate (PMA), or tumor necrosis factor-α (TNF-α) for 24 hr. The MUC5AC mucin gene expression was measured by reverse transcriptionpolymerase chain reaction. Production of MUC5AC mucin protein was measured by enzyme-linked immunosorbent assay. To elucidate the action mechanism of obtusifolin, effect of obtusifolin on PMA-induced nuclear factor kappa B (NF-κB) signaling pathway was investigated by western blot analysis. Obtusin, obtusifolin, or cassiaside inhibited the expression of MUC5AC mucin gene and the production of MUC5AC mucin protein, induced by EGF, PMA, or TNF-α. Obtusifolin inhibited PMA-induced activation (phosphorylation) of inhibitory kappa B kinase, and thus phosphorylation and degradation of inhibitory kappa B alpha. Obtusifolin inhibited PMA-induced nuclear translocation of NF-κB p65. These results suggest that obtusifolin can regulate the production and gene expression of mucin by acting on airway epithelial cells through regulation of NF-κB signaling pathway.

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Obtusifolin isolated from the seeds of Cassia obtusifolia regulates the gene expression and production of MUC5AC mucin in airway epithelial cells via affecting NF‐κB pathway

Kim

et al. 2019

Phytotherapy Research

Self Cite

“…[14][15][16] 17 Compounds from the root of Asparagus cochinchinensis (dioscin and methylprotodioscin) were reported in vitro to inhibit the expression of MUC5AC mucin protein in the NCI-H292 epithelial cells, which partially validatestraditional usesin inhibiting hypersecretion in the mucus of pulmonary tracts. 18 Asparagus gum polysaccharide have immune-modulatory functions and were shown to accelerate apoptosis, diminishing tumor development of hepatocellular carcinoma in liver. Cladophyll and roots of Asparagus in vivo were reported to lower diastolic and diastolic blood pressure, left cardio-ankle vascular index score, total cholesterol level, and blood glucose.…”

Section: Introductionmentioning

confidence: 99%

In vitro evaluation of anti-inflammatory and antioxidant effects of Asparagus aphyllus L., Crataegus azarolus L., and Ephedra alata Decne.in monocultures and co-cultures of HepG2 and THP-1-derived macrophages

Kmail¹,

Lyoussi²,

Zaid³

et al. 2017

Introduction: Asparagus aphyllus L.,Crataegus azarolus L., and Ephedra alata Decne.have been used in traditional Greco-Arab herbal medicine to treat various metabolic and inflammatory diseases. However, to our knowledge, no scientific evidences regarding their anti-inflammatory effects have been published yet.This study aimedto evaluate the antioxidant and anti-inflammatory properties of Asparagus aphyllus L., Crataegus azarolus L., and Ephedra alata Decne.extracts using in vitro mono and co-culture systems. Methods: In the first phase of the current study we measured the total phenol, flavones and flavonol contents as well as the antioxidant activity using the DPPH scavenging assay, ABTS assay, and reducing power of the three plant extracts. In second phase, we used THP-1-derived macrophages as a monoculture and co-culture with hepatic cell line (HepG2) to assess the effects of the three plant extracts on the production of LPSinduced pro-inflammatory (IL-6 and TNF -) and anti-inflammatory (IL-10) cytokines.Results: All three extracts exhibit relatively high levels of phenolics, flavones and flavonols, which correlate with their antioxidant capacities.All three extracts were found to modulate the secretion levels of TNF -, IL-6 and IL-10 at non-toxic concentrations as measured with MTT and LDH assays. Conclusion: Taken together, our results suggest that the traditional uses of these extracts as anti-inflammatory remedies may be mediated by their significant antioxidant capacity, inhibition of pro-inflammatory cytokines secretion concomitant with enhancement of anti-inflammatory cytokines secretion.Key words: Anti-inflammatory, Antioxidant; Asparagus aphyllus L.; Crataegus azarolus L.;Ephedra alata Decne. INTRODUCTIONInflammation is the first response of the immune system to infection, irritation, as well as to various other diseases such as cancer, cardiovascular diseases, obesity, hypertension, diabetes, autoimmune, and neurodegenerative disorders.1 it involves directed migration and activation of leukocytes (neutrophils, monocytes and eosinophils) to the site of damage. Inflammation is linked to oxidant/antioxidant imbalance (an elevation in the reactive oxygen speciesin parallel to low levels of antioxidants). High levels of inflammatory markers and low level of antioxidants are observed in many inflammatory diseases 2,3 as well as in many pathological conditions such as malignancy, cardiovascular disease, diabetes type 2, kidney malfunction, gastrointestinal disorders, microbial infection, fibrogenesis, and neurological diseases. The imbalance between production of antioxidants versus getting rid of free radical species leads to oxidative stress. [4][5][6] Inflammation is driven by the release of a wide range of pro-inflammatory mediators, such as the pro-inflammatory cytokines IL-1β, IL-TNF-α, IL-6, IL 8, and IFN-γ. Anti-inflammatory cytokines that includes IL-1 receptor antagonist, IL-4, IL-6, IL-10, IL-11, and IL-13 down regulate the inflammatory response. Chemo-therapeutic anti-inflammatory dr...

“…13 Methyl protodioscin from A. cochinchinensis also showed strong inhibitory action on the ALI model by oral administration.…”

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confidence: 97%

“…Dioscin and methyl protodioscin as major ingredients have been reported to inhibit mucin production. 13 Recently, we established the inhibitory action of the extract and its major constituent, methyl protodioscin, against lung inflammatory response by oral administration.…”

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confidence: 99%

The New Phytoformula ContainingMorus alba,Schizandra sinensisandAsparagus cochinchinensisInhibits Lung Inflammation in vitro and in vivo

et al. 2016

− A phytoformula containing the root barks of Morus alba, the fructus of Schizandra sinensis and the roots of Asparagus cochinchinensis (MSA) was prepared as a potential new herbal remedy, and its therapeutic potential for alleviating inflammatory lung conditions was examined. For in vivo evaluation, an animal model of lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice was used. With oral administration of 6 -60 mg/kg, MSA potently and dose-dependently inhibited bronchitis-like symptoms in acute lung injury induced by intranasal treatment of LPS as judged by the number of cells in the bronchoalveolar lavage fluid (BALF) and histological observation. The inhibitory potency was comparable with that of dexamethasone. For in vitro assay, the effects on the production of proinflammatory molecules in lung epithelial cells and alveolar macrophages were examined. Although MSA inhibited IL-6 production in IL-1β-treated lung epithelial cells (A549) only at a high concentration (300 μg/ml), the formula strongly and concentration-dependently inhibited NO production in LPStreated alveolar macrophages (MH-S) at 20 -300 μg/ml. Based on all of these findings, the new phytoformula MSA is suggested to have the potential to control inflammatory lung diseases including bronchitis, at least in part, by inhibiting inducible nitric oxide synthase-catalyzed NO production.