Diosgenin quantification by HPLC in a Dioscorea polygonoides tuber collection from colombian flora

Niño, Jaime; Jiménez, Diego A.; Mosquera, Oscar M.; Correa, Yaned M.

doi:10.1590/s0103-50532007000500030

Cited by 29 publications

(19 citation statements)

References 16 publications

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“…[113]. The quantification of diosgenin by HPLC-DAD-UV has also been accomplished in a Dioscorea polygonoides tuber collection from Colombian Flora [114]. Recently, an HPLC-UV method was applied to quantify diosgenin in aqueous extracts of fenugreek seeds aiming to support the preparation of the extracts and to standardize the diosgenin levels for further use in studies of the diosgenin potential antifertility effects [115].…”

Section: Methodsmentioning

confidence: 99%

Diosgenin: Recent Highlights on Pharmacology and Analytical Methodology

Jesus

Martins

Gallardo

et al. 2016

Journal of Analytical Methods in Chemistry

209

125

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Diosgenin, a steroidal sapogenin, occurs abundantly in plants such as Dioscorea alata, Smilax China, and Trigonella foenum graecum. This bioactive phytochemical not only is used as an important starting material for the preparation of several steroidal drugs in the pharmaceutical industry, but has revealed also high potential and interest in the treatment of various types of disorders such as cancer, hypercholesterolemia, inflammation, and several types of infections. Due to its pharmacological and industrial importance, several extraction and analytical procedures have been developed and applied over the years to isolate, detect, and quantify diosgenin, not only in its natural sources and pharmaceutical compositions, but also in animal matrices for pharmacodynamic, pharmacokinetic, and toxicological studies. Within these, HPLC technique coupled to different detectors is the most commonly analytical procedure described for this compound. However, other alternative methods were also published. Thus, the present review aims to provide collective information on the most recent pharmacological data on diosgenin and on the most relevant analytical techniques used to isolate, detect, and quantify this compound as well.

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Section: Methodsmentioning

confidence: 99%

Diosgenin: Recent Highlights on Pharmacology and Analytical Methodology

Jesus

Martins

Gallardo

et al. 2016

Journal of Analytical Methods in Chemistry

209

125

View full text Add to dashboard Cite

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“…A elucidação de métodos para a transformação da diosgenina em hormônios foi um marco relevante para a indústria farmacêutica (Chaturvedi, 1979;Zullo et al, 1987;Niño et al, 2007) e, atualmente, a maior parte do material coletado em regiões de ocorrência das espécies do gênero, tem como finalidade o fornecimento de matéria-prima para a extração dessa substância (Zullo et al, 1987;Itharat et al, 2004;Sautour et al, 2004;Niño et al, 2007;Olayemi & Ciênc. agrotec., Lavras, v. 35, n. 1, p. 92-98, jan./fev.…”

Section: Introductionunclassified

Micropropagação de Dioscorea multiflora Grised

et al. 2011

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Dioscorea multiflora uma planta nativa do Sul do Brasil produz a diosgenina como metabólito secundário majoritário, uma substância potencialmente usada pela indústria farmacêutica para a produção de cortisona e substâncias com ação contraceptiva. Objetivou-se, neste trabalho otimizar o protocolo de micropropagação de D. multiflora, visando a produção de mudas em escala comercial. Segmentos nodais subcultivados em meio MS sólido foram transferidos para multiplicação em meio MS suplementado com BAP (0,01; 0,1; 0,5; 1,0 e 3,0 mg L-1)e meio MS suplementado com 0,1 mg L-1 ou 0,5 mg L-1 de BAP acrescido de diferentes concentrações de sacarose (2, 4, 6, 8 e 10%). Para o enraizamento, as brotações foram cultivadas em meio MS suplementado com AIB (0,1; 0,5; 1,0 e 3,0 mg L-1) e meio MS suplementado com ANA (0,1; 0,5; 1,0 e 3,0 mg L-1). Os experimentos in vitro foram instalados em delineamento experimental inteiramente casualizado e cada tratamento constituiu-se de 3 repetições e 10 cubetas/parcela. Plântulas com e sem raízes foram aclimatizadas em casa de vegetação. Melhores resultados de multiplicação e enraizamento foram obtidos em meio MS + 0,1 mg L-1 de BAP (80%) e em meio MS + 1,0 mg L-1 de AIB (42,6%), respectivamente. Não houve diferença quanto à porcentagem de sobrevivência das plântulas in vitro e ex vitro durante a aclimatização (75%). O protocolo de micropropagação para D. Multiflora é efetivo e pode ser usado para a produção em escala comercial.

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“…The experimental conditions were isocratic binary system of acetonitrile/water (90:10) at a flow rate of 1 mL min -1 (Jaime Niño et al 2007). …”

Section: Diosgenin Quantificationmentioning

confidence: 99%

Effect of endophytic Bacillus sp. from selected medicinal plants on growth promotion and diosgenin production in Trigonella foenum-graecum

Jasim

Geethu

Mathew

et al. 2015

Plant Cell Tiss Organ Cult

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Endophytic microorganisms support the growth of host plants by production of phytohormones, N 2 fixation, synthesis of ACC deaminase, phosphate solubilization and also by means of production of antimicrobial metabolites or siderophores. In the present study, effect of endophytic Bacillus sp. was analysed on the growth enhancement and metabolite modulation in the seedlings of Fenugreek (Trigonella foenum-graecum L.), a well known spice with medicinal properties. Preliminary screening has resulted in the identification of strains CaB 1 (Bacillus cereus), CaB 2 (Bacillus thuringiensis) and CaB 5 (Bacillus sp.) isolated from Capsicum annuum and EcB 4 (Bacillus sp.) isolated from Elettaria cardamomum to have excellent effect on seedling growth enhancement in Fenugreek. The plantlets formed from the treatment were further analysed for leaf number, shoot length, root length, wet weight and diosgenin concentration, which is the principal secondary metabolite of the plant. Interestingly, HPLC analysis has confirmed the ability of CaB 5 to induce highest level of diosgenin biosynthesis (527.83 ± 13.5 lg/mL). The obtained results are of great significance as it widens the exploration of endophytic bacteria on enhanced synthesis of medicinal natural products.

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Diosgenin quantification by HPLC in a Dioscorea polygonoides tuber collection from colombian flora

Cited by 29 publications

References 16 publications

Diosgenin: Recent Highlights on Pharmacology and Analytical Methodology

Diosgenin: Recent Highlights on Pharmacology and Analytical Methodology

Micropropagação de Dioscorea multiflora Grised

Effect of endophytic Bacillus sp. from selected medicinal plants on growth promotion and diosgenin production in Trigonella foenum-graecum

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