Dipeptidyl peptidase-IV expression and activity in human glomerular endothelial cells

High Fat and High Cholesterol Diet Induces DPP-IV Activity in Intestinal Lymph

“…Our results suggest that dyslipidemia may reduce incretin concentrations, which directly links dyslipidemia with type 2 diabetes. Others have noted that expression of DPP-IV is increased after consumption of a high fat diet 5 7 , and several relevant cell types, including vascular endothelial cells 16 and adipocytes 7 , have been shown to express DPP-IV. Therefore, further experiments are required to elucidate the molecular mechanisms and tissues responsible for high fat and high cholesterol diet-induced up-regulation of DPP-IV expression.…”

Hyperglycaemia increases dipeptidyl peptidase IV activity in diabetes mellitus

“…It should be observed that DPP-IV is expressed at the surface of endothelial cells [15] and that circulating enzyme accounts for only a fraction of total DPP-IV activity. Furthermore, differences in structure and function between circulating and endothelial DPP-IV, and the contribution of each component to incretin degradation, are not known in detail.…”

“…To evaluate within-and between-run precision of the DPP-IV assay, the activities of serum samples with low (15 U/l), middle (30 U/l) and high (70 U/l) activity were assessed ten times in 5 days. The coefficients of variation were 2.6, 3.4 and 2.3%, respectively for within-run precision and 1.5, 4.8 and 4.7%, respectively for between-run precision [15].…”

“…In fact, circulating DPP-IV levels were reported to be reduced in a small sample of elderly patients with type 2 diabetes [14]; in a study on another small sample of subjects, no significant difference in circulating enzyme activity was detected between type 2 diabetic patients and matched healthy controls [5]. On the other hand, DPP-IV mRNA expression and enzyme activity have been reported to be stimulated by hyperglycaemia in human endothelial cells in vitro [15]. In order to elucidate the relationships between chronic hyperglycaemia and DDP-IV in diabetes, enzyme activity was studied in blood samples from patients with type 1 and type 2 diabetes, compared with subjects with IGT or NGT.…”

Plasma dipeptidyl peptidase 4 activity correlates with body mass index and the plasma adiponectin concentration in healthy young people

“…Clinical studies have been reported that DPP4 activity showed significant correlations with plasma glucose and HbA1c levels in diabetic patients [9,10]. Moreover, an in vitro study determined that exposure to high levels of glucose enhanced the biosynthesis of DPP4 in human glomerular endothelial cells [20]. Pala et al reported that oral glucose loading did not induce DPP4 activation in normal, impaired glucose tolerance, or type 2 diabetes subjects [21].…”