Dipolar-Based Torsion Angle Measurements for Protein Structure Determination

Ladizhansky, Vladimir

doi:10.1002/9780470034590.emrstm1153

Cited by 4 publications

(6 citation statements)

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“…Their accurate site-specific measurement in macromolecules under MAS has been rather challenging, and multiple groups in the field have turned their attention to this problem, as discussed in recent reviews [34, 43, 44]. …”

Section: Sample Preparation and Mas Nmr Methods For Structural Anamentioning

confidence: 99%

MAS NMR of HIV-1 protein assemblies

Suiter

Quinn

et al. 2015

Journal of Magnetic Resonance

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The negative global impact of the AIDS pandemic is well known. In this perspective article, the utility of magic angle spinning (MAS) NMR spectroscopy to answer pressing questions related to the structure and dynamics of HIV-1 protein assemblies is examined. In the recent years, MAS NMR has undergone major technological developments enabling studies of large viral assemblies. We discuss some of these evolving methods and technologies and provide a perspective on the current state of MAS NMR as applied to the investigations into structure and dynamics of HIV-1 assemblies of CA capsid protein and of Gag maturation intermediates.

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Section: Sample Preparation and Mas Nmr Methods For Structural Anamentioning

confidence: 99%

MAS NMR of HIV-1 protein assemblies

Suiter

Quinn

et al. 2015

Journal of Magnetic Resonance

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“…This illustrates one common challenge of torsion angle experiments, which is that they often have regions of angular space where the obtained dephasing curves are essentially indistinguishable. As reviewed in earlier work ( Hong, 2006 ; Hong and Wi, 2006 ; Ladizhansky, 2009 ), this issue can be addressed by combining multiple types of dihedral angle measurements with complementary sensitivities. Notably, the NCCN experiment minima are close to both the X-ray structure angles and the results of NCCN-based chemical shift analysis.…”

Section: Ssnmr Torsion Angle Measurements—nccn As An Examplementioning

confidence: 99%

“…The first examples of direct MAS ssNMR torsion angle measurements were the HCCH experiments ( Feng et al, 1996 ; Feng et al, 1997b ), whose principles were also discussed in some detail in prior reviews ( Hong and Wi, 2006 ; Ladizhansky, 2009 ). Briefly, again a DQ state is generated between two directly bonded 13 C carbons, but now it is combined with controlled recoupling of the C-H dipolar interaction ( Figure 4A ).…”

Section: Beyond the Protein Backbone—hcch Experimentsmentioning

confidence: 99%

“…In this review article we examine MAS ssNMR techniques that allow direct insights into angular constraints that define the structures of proteins and other biomolecules. Readers are also referred to earlier reviews in the mid 2000s ( Hong, 2006 ; Hong and Wi, 2006 ; Ladizhansky, 2009 ), and the current review will focus on more recent applications since 2006. We shall also examine the place of these techniques in current and future biomolecular MAS ssNMR.…”

Section: Introductionmentioning

confidence: 99%

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Dihedral Angle Measurements for Structure Determination by Biomolecular Solid-State NMR Spectroscopy

Wel

2021

Front. Mol. Biosci.

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In structural studies of immobilized, aggregated and self-assembled biomolecules, solid-state NMR (ssNMR) spectroscopy can provide valuable high-resolution structural information. Among the structural restraints provided by magic angle spinning (MAS) ssNMR the canonical focus is on inter-atomic distance measurements. In the current review, we examine the utility of ssNMR measurements of angular constraints, as a complement to distance-based structure determination. The focus is on direct measurements of angular restraints via the judicious recoupling of multiple anisotropic ssNMR parameters, such as dipolar couplings and chemical shift anisotropies. Recent applications are highlighted, with a focus on studies of nanocrystalline polypeptides, aggregated peptides and proteins, receptor-substrate interactions, and small molecule interactions with amyloid protein fibrils. The review also examines considerations of when and where ssNMR torsion angle experiments are (most) effective, and discusses challenges and opportunities for future applications.

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“…Consequently, there are multidimensional NMR experiments that precisely measure site-specific CSAs (Wylie and Rienstra 2008). There are alternative ways to measure dihedral angles, for example, by correlating CSA or dipolar tensors (Ladizhansky 2010).…”

Section: Structural Informationmentioning

confidence: 99%