Direct Analysis of Doping Agents in Raw Urine Using Hydrophobic Paper Spray Mass Spectrometry

Rossini, Eduardo Luiz; Kulyk, Dmytro S.; Ansu-Gyeabourh, Emelia; Sahraeian, Taghi; Pezza, Helena Redigolo; Badu‐Tawiah, Abraham K.

doi:10.1021/jasms.0c00063

Cited by 38 publications

(39 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The study furthermore combined the analysis with field asymmetric ion mobility spectrometry (FAIMS) to further improve assay sensitivity and selectivity. Rossini et al took a similar approach, applying hydrophobic PSI to the detection of doping agents in raw urine 54 . In this study, the paper surface was treated with trichloromethylsilane to improve the ionization of target analytes, specifically the anabolic agents clenbuterol and trenbolone, and the diuretics furosemide and hydrochlorothiazide.…”

Section: Applications Of Ambient Ionization Mass Spectrometrymentioning

confidence: 99%

Applications of ambient ionization mass spectrometry in 2020: An annual review

Rankin‐Turner

Heaney

2021

Analytical Science Advances

View full text Add to dashboard Cite

Recent developments in mass spectrometry (MS) analyses have seen a concerted effort to reduce the complexity of analytical workflows through the simplification (or removal) of sample preparation and the shortening of run‐to‐run analysis times. Ambient ionization mass spectrometry (AIMS) is an exemplar MS‐based technology that has swiftly developed into a popular and powerful tool in analytical science. This increase in interest and demonstrable applications is down to its capacity to enable the rapid analysis of a diverse range of samples, typically in their native state or following a minimalistic sample preparation approach. The field of AIMS is constantly improving and expanding, with developments of powerful and novel techniques, improvements to existing instrumentation, and exciting new applications added with each year that passes. This annual review provides an overview of applications of AIMS techniques over the past year (2020), with a particular focus on the application of AIMS in a number of key fields of research including biomedical sciences, forensics and security, food sciences, the environment, and chemical synthesis. Novel ambient ionization techniques are introduced, including picolitre pressure‐probe electrospray ionization and fiber spray ionization, in addition to modifications and improvements to existing techniques such as hand‐held devices for ease of use, and USB‐powered ion sources for on‐site analysis. In all, the information provided in this review supports the view that AIMS has become a leading approach in MS‐based analyses and that improvements to existing methods, alongside the development of novel approaches, will continue across the foreseeable future.

show abstract

Section: Applications Of Ambient Ionization Mass Spectrometrymentioning

confidence: 99%

Applications of ambient ionization mass spectrometry in 2020: An annual review

Rankin‐Turner

Heaney

2021

Analytical Science Advances

View full text Add to dashboard Cite

show abstract

“…On the other hand, low-polarity analytes interact less with the cellulosic paper, which favors their elution while improving analyte ionization. Chemical modifications of the paper surface can help improve the efficiency of PSI and increase analyte-specific sensitivity (Damon et al, 2016;Filho et al, 2020;Rossini et al, 2020).…”

Section: Type Of Chemistrymentioning

confidence: 99%

Ambient ionization mass spectrometry applied to new psychoactive substance analysis

Ena

Cowan

Abbate

2021

Mass Spectrometry Reviews

View full text Add to dashboard Cite

In the past decade a plethora of drugs with similar effects to controlled psychoactive drugs, like cannabis, amfetamine (amphetamine), or lysergic acid diethylamide, have been synthesized. These drugs can collectively be classified under the term new psychoactive substances (NPS) and are used for recreational purposes. The novelty of the substances, alongside the rapid rate of emergence and structural variability, makes their detection as well as their legal control highly challenging, increasing the demand for rapid and easy-to-use analytical techniques for their detection and identification. Therefore, interest in ambient ionization mass spectrometry applied to NPS has grown in recent years, which is largely because it is relatively fast and simple to use and has a low operating cost. This review aims to provide a critique of the suitability of current ambient ionization techniques for the analysis of NPS in the forensic and clinical toxicology fields. Consideration is given to analytical performance and ease of implementation, including ionization efficiency, selectivity, sensitivity, quantification, analyte chemistry, molecular coverage, validation, and practicality.

show abstract

“…While the masking properties of diuretics are today significantly more limited than 30 years ago, diuretics are still considered as potentially performance‐enhancing in selected sports 153 . In the light of comparably high MRPL of 200 ng/ml established for most diuretics 51 and the xenobiotic nature of the analytes, omitting chromatographic separation has been considered as viable option, and Rossini et al demonstrated the capability of paper spray MS with surface‐modified grade‐I filter paper to allow for the detection of furosemide and hydrochlorothiazide in spiked urine samples at low ng/ml concentrations 154 . A volume of 6 μl of urine was spotted onto a trichlorosilane‐derivatized paper triangle interfaced to a linear ion trap MS system, and target compounds were detected in MS/MS mode, offering an alternative analytical ITP approach for a subset of target compounds in doping controls if adequate reproducibility and robustness is proven.…”

Section: Diuretics Other Masking Agents and Stimulantsmentioning

confidence: 99%

Annual banned‐substance review: Analytical approaches in human sports drug testing 2019/2020

Thevis

Kuuranne

Geyer

2020

Drug Testing and Analysis

View full text Add to dashboard Cite

Analytical chemistry-based research in sports drug testing has been a dynamic endeavor for several decades, with technology-driven innovations continuously contributing to significant improvements in various regards including analytical sensitivity, comprehensiveness of target analytes, differentiation of natural/ endogenous substances from structurally identical but synthetically derived compounds, assessment of alternative matrices for doping control purposes, and so forth. The resulting breadth of tools being investigated and developed by anti-doping researchers has allowed to substantially improve anti-doping programs and data interpretation in general. Additionally, these outcomes have been an extremely valuable pledge for routine doping controls during the unprecedented global health crisis that severely affected established sports drug testing strategies. In this edition of the annual banned-substance review, literature on recent developments in antidoping published between October 2019 and September 2020 is summarized and discussed, particularly focusing on human doping controls and potential applications of new testing strategies to substances and methods of doping specified the World Anti-Doping Agency's 2020 Prohibited List.

show abstract

Direct Analysis of Doping Agents in Raw Urine Using Hydrophobic Paper Spray Mass Spectrometry

Cited by 38 publications

References 66 publications

Applications of ambient ionization mass spectrometry in 2020: An annual review

Applications of ambient ionization mass spectrometry in 2020: An annual review

Ambient ionization mass spectrometry applied to new psychoactive substance analysis

Annual banned‐substance review: Analytical approaches in human sports drug testing 2019/2020

Contact Info

Product

Resources

About