Direct analysis of metabolites in the liver tissue of zebrafish exposed to fiproles by internal extractive electrospray ionization mass spectrometry

Abstract

Exploring the metabolic disturbance of fipronil and its derivatives in aquatic organisms may provide a more comprehensive understanding of the impact of fipronil on ecological environment. In this work, internal...

Liver Metabolic Dysregulation Induced by Polypropylene Nano- and Microplastics in Nile Tilapia Using Internal Extractive Electrospray Ionization Mass Spectrometry

“…Though several sensitive detection means were developed, finding out the biomarkers after the action of environmental pollutants on the living organism is still one challenge in the field of environmental toxicology. 14 Mass spectrometry (MS) has become an important platform, analyzing the effects of exogenous pollutants on biological metabolism at the molecular level. In nontargeted metabonomics analysis, traditional mass spectrometry analysis methods such as liquid chromatography-MS (LC-MS) and gas chromatography-MS (GC-MS) typically require labor-intensive pretreatment procedures like grinding, extraction, and separation.…”

“…In nontargeted metabonomics analysis, traditional mass spectrometry analysis methods such as liquid chromatography-MS (LC-MS) and gas chromatography-MS (GC-MS) typically require labor-intensive pretreatment procedures like grinding, extraction, and separation. 14,15 result, those conventional means are comparatively timeconsuming, unable to meet the actual needs of highthroughput analysis for numerous samples, and inevitably lead to the loss of some active components in tissue samples. 15−17 To some extent, it limits the development of environmental metabonomics and environmental toxicology.…”

“…Though several sensitive detection means were developed, finding out the biomarkers after the action of environmental pollutants on the living organism is still one challenge in the field of environmental toxicology . Mass spectrometry (MS) has become an important platform, analyzing the effects of exogenous pollutants on biological metabolism at the molecular level.…”

“…Mass spectrometry (MS) has become an important platform, analyzing the effects of exogenous pollutants on biological metabolism at the molecular level. In nontargeted metabonomics analysis, traditional mass spectrometry analysis methods such as liquid chromatography-MS (LC-MS) and gas chromatography-MS (GC-MS) typically require labor-intensive pretreatment procedures like grinding, extraction, and separation. , As a result, those conventional means are comparatively time-consuming, unable to meet the actual needs of high-throughput analysis for numerous samples, and inevitably lead to the loss of some active components in tissue samples. − To some extent, it limits the development of environmental metabonomics and environmental toxicology . Therefore, the development of new mass spectrometry techniques for rapid, efficient, accurate, real-time, in situ, and online analysis of metabolites in complex biological samples has become a new requirement for the construction of environmental metabolomics and environmental toxicology.…”

Internal extractive electrospray ionization mass spectrometry for investigating the phospholipid dysregulation induced by perfluorooctanoic acid in Nile tilapia

“…[25][26][27][28][29][30] Internal extractive electrospray ionization mass spectrometry (iEESI-MS) is a typical AMS technique, which can simultaneously extract and ionize endogenous metabolites in tissue samples without any sample preparation. [31][32][33][34][35] Herein, we applied iEESI-MS for direct phospholipid profiling in Nile tilapia liver and spleen tissue samples and investigating potential phospholipid dysregulation induced by PFOA in aquatic organisms (Fig. 1).…”