DIRECT AND INDIRECT SOMATIC EMBRYOGENESIS ON ARABICA COFFEE (Coffea arabica)

Abstract

Propagation of Coffea arabica L. through direct and indirect somatic embryogenesis technique is promising for producing large number of coffee seedlings. The objectives of the research were to evaluate methods for direct and indirect somatic embryogenesis induction of C. arabica var. Kartika. The explants were the youngest fully expanded leaves of arabica coffee. The evaluated medium was modified Murashige and Skoog (MS) medium supplemented with a combination of 2.26 µ M 2,4-D + 4.54 or 9.08 µ M thidiazuron; 4.52 µ M 2,4-D + 4.54 or 9.08 µ M thidiazuron; or 9.04 µ M 2,4-D + 9.08 µ M thidiazuron. Both calli (100 mg) and pre-embryos developed from the edge of leaf explants were subcultured into regeneration medium (half strength MS with modified vitamin, supplemented with kinetine 9.30 µ M and adenine sulfate 40 mg L -1 ). The results showed coffee leaf explant cultured on medium containing 2.26 µ M 2,4-D + 4.54 or 9.08 µ M thidiazuron to induce direct somatic embriogenesis from explant, while that of 4.52 or 9.04 µM 2,4-D + 9.08 µ M thidiazuron to induced indirect somatic embriogenesis. The medium for calli induction from coffee by explants was medium supplemented with 4.52 or 9.04 µ M 2,4-D in combination with 9.08 µ M thidiazuron. On the other hand, the best medium for activation of induction of somatic embryos was MS medium supplemented with 9.04 µ M 2,4-D + 9.08 µ M thidiazuron. Based on this results, the first step for developing micropropagation for coffee has been resolved. The subsequent studies will be directed to evaluate agronomic performance of the derived planting materials.

EFFECTIVE CACAO SOMATIC EMBRYO REGENERATION ON KINETIN SUPPLEMENTED DKW MEDIUM AND SOMACLONAL VARIATION ASSESSMENT USING SSRs MARKERS

“…In vitro plantlet regeneration via somatic embryos has been developed in many plants species, such as coffee (Ibrahim et al, 2012;Ibrahim et al, 2013a;Ibrahim et al, 2013b), soybean (Widoretno et al, 2003a) and one of the rare Indonesian medicinal plants (purwoceng -Pimpinella pruatjan Molk.) (Ajijah et al, 2010).…”

DESDIFERENCIACIÓN CELULAR IN VITRO DE Coffea arabica L. “CAFÉ” VAR. CATURRA A PARTIR DE EXPLANTES FOLIARES

“…Los resultados del ensayo de iniciación de callo indican que la auxina 2,4-D, a una concentración de 0,5 mg/l, induce la formación de callo friable, y la combinación de 6-bencilaminopurina (6-BAP) incrementa la intensidad de proliferación estimulando el desarrollo de embriones somáticos, esto es corroborado con los resultados de Moncada et al (2004), Ibrahim et al (2013) y Sánchez Jhong et al (2019). Sin embargo, utilizando como fitohormonas 2,4-D y Kinetina se obtuvo mayor porcentaje de callo compacto (Fig.…”

EFISIENSI MEDIA KULTUR DAN APLIKASI TEMPORARY IMMERSION SYSTEM PADA EMBRIOGENESIS SOMATIK KOPI ARABIKA / Efficiency of Culture Media and Aplication Temporary Immersion Systemon on Somatic Embryogenesis Arabica Coffee

“…Rezende et al (2012) menggunakan phytagel sebanyak 3,4 g L -1 pada kultur in vitro kopi. Ibrahim et al (2015), Ibrahim et al (2013) bacto agar untuk induksi kalus dan 3,0 g L -1 Gelrite dalam perkembangan embrio somatik kopi.…”