Direct Bacterial Killing In Vitro by Recombinant Nod2 Is Compromised by Crohn's Disease-Associated Mutations

The c-Jun N-terminal Kinase (JNK)-binding Protein (JNKBP1) Acts as a Negative Regulator of NOD2 Protein Signaling by Inhibiting Its Oligomerization Process

“…The NOD2-mediated antibacterial response involves two kinds of mechanisms: direct bacterial killing mechanisms, such as those involving autophagy and reactive oxygen species production (12,13,24,32), and indirect mechanisms, which depend on the expression of NF-Btarget proinflammatory and antibacterial genes (14,33,34). The NOD2 anti-bacterial effect monitored by the gentamicin protection assay after L. monocytogenes infection for 2 h results more probably from a direct killing, such as autophagy.…”

“…1E). The efficiency of MDP treatment was demonstrated by immunoblot against IB␣ phosphorylated on Ser 32 and Ser 36 in whole cell lysates (Fig. 1, D and E).…”

“…HCT116 cells transfected with siRNA Ctrl or siRNA JNKBP1 (siRNA 4 or 6) for 48 h were stimulated with MDP for the indicated time. Lysates were collected and immunoblotted for phosphorylated IB␣ on Ser 32 and Ser 36 , IKK␣/␤ on Ser 176 and Ser…”