Direct biosynthesis of adipic acid from lignin-derived aromatics using engineered Pseudomonas putida KT2440

Niu, Wei; Willett, Howard; Mueller, Jenna L.; He, Xinyuan; Kramer, Levi; Ma, Bin; Guo, Jiantao

doi:10.1016/j.ymben.2020.02.006

Cited by 52 publications

(32 citation statements)

References 42 publications

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“…[209] Complete lignin valorization chains using cis,cis-muconate-producing P. putida KT2440 as biocatalysts yielding bio-nylon were described by Vardon et al [209] and Kohlstedt et al [208] Recently, a completely bio-based transformation of lignin-derivable monomers (4-coumarate, ferulate, 4hydroxybenzoate) to adipate was demonstrated using P. putida KT2440 by introducing an artificial pathway that converts the naturally occurring degradation intermediate -ketoadipate into adipate with titers and yields up to 17.4 mm and 18.4% mol mol −1 . [210] This process shows a high potential but is currently somewhat limited by by-product formation. [210] The popularity of cis,cis-muconate is related to its value but also because it can be derived from many different substrates.…”

Section: Application Of Biotransformation For Production Of Aromaticsmentioning

confidence: 99%

“…[ 210 ] This process shows a high potential but is currently somewhat limited by by‐product formation. [ 210 ] The popularity of cis,cis ‐muconate is related to its value but also because it can be derived from many different substrates. In Pseudomonas , cis,cis ‐muconate is a natural intermediate of benzoate, phenol, and catechol degradation.…”

Section: Biotransformationmentioning

confidence: 99%

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Pseudomonas as Versatile Aromatics Cell Factory

Schwanemann

Otto

Wierckx

et al. 2020

Biotechnology Journal

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Aromatics and their derivatives are valuable chemicals with a plethora of important applications and thus play an integral role in modern society. Their current production relies mostly on the exploitation of petroleum resources. Independency from dwindling fossil resources and rising environmental concerns are major driving forces for the transition towards the production of sustainable aromatics from renewable feedstocks or waste streams. Whole‐cell biocatalysis is a promising strategy that allows the valorization of highly abundant, low‐cost substrates. In the last decades, extensive efforts are undertaken to allow the production of a wide spectrum of different aromatics and derivatives using microbes as biocatalysts. Pseudomonads are intriguing hosts for biocatalysis, as they display unique characteristics beneficial for the production of aromatics, including a distinct tolerance and versatile metabolism. This review highlights biotechnological applications of Pseudomonas as host for the production of aromatics and derived compounds. This includes their de novo biosynthesis from renewable resources, biotransformations in single‐ and biphasic fermentation setups, metabolic funneling of lignin‐derived aromatics, and the upcycling of aromatic monomers from plastic waste streams. Additionally, this review provides insights into unique features of Pseudomonads that make them exceptional hosts for aromatics biotechnology and discusses engineering strategies.

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Section: Application Of Biotransformation For Production Of Aromaticsmentioning

confidence: 99%

Section: Biotransformationmentioning

confidence: 99%

Pseudomonas as Versatile Aromatics Cell Factory

Schwanemann

Otto

Wierckx

et al. 2020

Biotechnology Journal

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“…Recently, the feasibility of direct de novo synthesis of adipic acid from lignin model compounds was demonstrated using P. putida KT2440 (Niu et al 2020 ). To this end, the pathway design profited from the C6 structure of the β -KA pathway intermediate 3-ketoadipoyl-CoA, i.e., there is no need to exploit a condensation reaction between an acetyl-CoA and a succinyl-CoA unit as previously reported for adipic acid biosynthesis using glucose- or glycerol-grown E. coli (Cheong et al 2016 ; Yu et al 2014 ; Zhao et al 2018 ).…”

Section: Bioproduction and Industrial Applicationmentioning

confidence: 99%

Industrial biotechnology of Pseudomonas putida: advances and prospects

Weimer

Kohlstedt

Volke

et al. 2020

Appl Microbiol Biotechnol

172

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Pseudomonas putida is a Gram-negative, rod-shaped bacterium that can be encountered in diverse ecological habitats. This ubiquity is traced to its remarkably versatile metabolism, adapted to withstand physicochemical stress, and the capacity to thrive in harsh environments. Owing to these characteristics, there is a growing interest in this microbe for industrial use, and the corresponding research has made rapid progress in recent years. Hereby, strong drivers are the exploitation of cheap renewable feedstocks and waste streams to produce value-added chemicals and the steady progress in genetic strain engineering and systems biology understanding of this bacterium. Here, we summarize the recent advances and prospects in genetic engineering, systems and synthetic biology, and applications of P. putida as a cell factory. Key points • Pseudomonas putida advances to a global industrial cell factory. • Novel tools enable system-wide understanding and streamlined genomic engineering. • Applications of P. putida range from bioeconomy chemicals to biosynthetic drugs. Electronic supplementary material The online version of this article (10.1007/s00253-020-10811-9) contains supplementary material, which is available to authorized users.

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“…[ 122–124 ] For example, glucose is converted to a range of intermediates or final products such as 5‐HMF, isosorbide, polylactic acid, and valeric acid via catalytic hydrogenation, dehydrogenation, dehydration, and oxidation in the presence of acid or metal‐supported catalysts such as Ru/C, Cu‐Ru/C, SnCl 2 , Amberlyst‐15 at 363–513 K. [ 122,123,125,126 ] Ethanol, lactic acid, succinic acid, and adipic acid are produced via biochemical pathways through fermentation. [ 127–129 ] These value‐added bioproducts are platform chemicals for various industries with a variety of applications, ranging from organic solvents to liquid fuels.…”

Section: Commercial Status: Application Of Sub/supercritical Fluids Imentioning

confidence: 99%

Conversion of Lignocellulosic Biomass to Reducing Sugars in High Pressure and Supercritical Fluids: Greener Alternative for Biorefining of Renewables

Kumar

Kermanshahi-pour

Brar

et al. 2021

Advanced Sustainable Systems

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Supercritical fluids offer great potential to be employed in lignocellulosic biomass (LCB) fractionation in biorefinery. Supercritical carbon dioxide and water are greener alternatives compared with conventional reagents and have been investigated for the pretreatment and hydrolysis of lignocellulosic biomass. This review is focused on examining the fundamentals that govern the function of supercritical fluids in the pretreatment stage, as well as in the main hydrolysis reaction. Sub/supercritical carbon dioxide is used in pretreatment and sub/supercritical water has been the solvent of choice in hydrolysis of LCB. Significant research has gone into understanding the effect of process parameters such as temperature, pressure, cosolvent, and use of external catalyst on the sugar yield in biorefining of the LCB in supercritical fluids. It is shown that processes with reduced environmental impact and energy consumption can significantly enhance biorefining of LCB at commercial scale. Enzymatic hydrolysis of LCB in supercritical carbon dioxide is a promising approach that can accommodate mild reaction conditions. Developing an understanding of the performance of enzymes in high pressure systems and designing carriers for enzyme immobilization and further recycling is expected to enable one pot pretreatment and hydrolysis and is an important milestone in processing renewable resources for deriving biofuel and value‐added chemicals.

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Direct biosynthesis of adipic acid from lignin-derived aromatics using engineered Pseudomonas putida KT2440

Cited by 52 publications

References 42 publications

Pseudomonas as Versatile Aromatics Cell Factory

Pseudomonas as Versatile Aromatics Cell Factory

Industrial biotechnology of Pseudomonas putida: advances and prospects

Conversion of Lignocellulosic Biomass to Reducing Sugars in High Pressure and Supercritical Fluids: Greener Alternative for Biorefining of Renewables

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