Direct competitive ELISA enhanced by dynamic light scattering for the ultrasensitive detection of aflatoxin B1 in corn samples

Zhan, Shengnan; Hu, Jing; Yu, Li; Huang, Xiaolin; Xiong, Yonghua

doi:10.1016/j.foodchem.2020.128327

Cited by 51 publications

(25 citation statements)

References 37 publications

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“…The output signal was enhanced via dynamic light scattering (DLS). This study used a similar mechanism of oxidising glucose by GOx to produce H 2 O 2 , which was then oxidised into ∙ OH by HRP [ 100 ]. The sensitivity of this method was enhanced by amplifying the scattering signals from aggregations of the gold nanoparticles (AuNPs) induced by the formation of ∙ OH [ 93 ].…”

Section: Conventional and Advanced Analytical Technologiesmentioning

confidence: 99%

“…The sensitivity of this method was enhanced by amplifying the scattering signals from aggregations of the gold nanoparticles (AuNPs) induced by the formation of ∙ OH [ 93 ]. The signal amplification achieved a LOD (10% inhibitory concentration, IC 10 ) of 0.00012 μg kg −1 , with a regression equation of y = 16.899 ln( x ) + 44.794 and an IC 50 of 0.00136 μg kg −1 , which are around 104- and 16-fold, respectively, lower than the aforementioned colorimetric ELISA [ 100 ].…”

Section: Conventional and Advanced Analytical Technologiesmentioning

confidence: 99%

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Recent Advances in Conventional Methods and Electrochemical Aptasensors for Mycotoxin Detection

Ong

Pike

Tan

2021

Foods

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The presence of mycotoxins in foodstuffs and feedstuffs is a serious concern for human health. The detection of mycotoxins is therefore necessary as a preventive action to avoid the harmful contamination of foodstuffs and animal feed. In comparison with the considerable expense of treating contaminated foodstuffs, early detection is a cost-effective way to ensure food safety. The high affinity of bio-recognition molecules to mycotoxins has led to the development of affinity columns for sample pre-treatment and the development of biosensors for the quantitative analysis of mycotoxins. Aptamers are a very attractive class of biological receptors that are currently in great demand for the development of new biosensors. In this review, the improvement in the materials and methodology, and the working principles and performance of both conventional and recently developed methods are discussed. The key features and applications of the fundamental recognition elements, such as antibodies and aptamers are addressed. Recent advances in aptasensors that are based on different electrochemical (EC) transducers are reviewed in detail, especially from the perspective of the diagnostic mechanism; in addition, a brief introduction of some commercially available mycotoxin detection kits is provided.

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Section: Conventional and Advanced Analytical Technologiesmentioning

confidence: 99%

Section: Conventional and Advanced Analytical Technologiesmentioning

confidence: 99%

Recent Advances in Conventional Methods and Electrochemical Aptasensors for Mycotoxin Detection

Ong

Pike

Tan

2021

Foods

View full text Add to dashboard Cite

show abstract

“…Indeed, H2O2-mediated TYR was used as signal amplification system. The DLS-ELISA exhibited a LOD as low as 0.12 pg•mL −1 which was about 153-and 385-folds lower than those of conventional plasmonic In another plasmonic ELISA, aggregation-induced color change of AuNPs, as a main strategy to regulate the plasmonic signal, was employed for the ultrasensitive detection of AFB1 using dynamic light scattering (DLS) signal instead of absorbance (Figure 4c) [61]. In the developed DLS-ELISA, GOx-AFB1 was used as competing antigen because the GOx can effectively convert glucose to H 2 O 2 .…”

Section: Enzyme-linked Immunosorbent Assay (Elisa)mentioning

confidence: 99%

“…(a) Colorimetric GOx-based ELISA using acid-base indicator bromocresol purple (BCP) for AFB1 detection; (b) Plasmonic ELISA based on the urease-induced metallization of gold nanoflowers for OTA detection; (c) DLS-ELISA method associated with H 2 O 2 -mediated tyramine signal amplification system for AFB1 detection. Reproduced from[58,60,61], respectively, with permission.…”

mentioning

confidence: 99%

Advances in Colorimetric Strategies for Mycotoxins Detection: Toward Rapid Industrial Monitoring

Majdinasab

Aissa

Marty

2020

Toxins

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Mycotoxins contamination is a global public health concern. Therefore, highly sensitive and selective techniques are needed for their on-site monitoring. Several approaches are conceivable for mycotoxins analysis, among which colorimetric methods are the most attractive for commercialization purposes thanks to their visual read-out, easy operation, cost-effectiveness, and rapid response. This review covers the latest achievements in the last five years for the development of colorimetric methods specific to mycotoxins analysis, with a particular emphasis on their potential for large-scale applications in food industries. Gathering all types of (bio)receptors, main colorimetric methods are critically discussed, including enzyme-linked assays, lateral flow-assays, microfluidic devices, and homogenous in-solution strategies. This special focus on colorimetry as a versatile transduction method for mycotoxins analysis is comprehensively reviewed for the first time.

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“…Inspired by the idea of “old drug in new use,” a number of well-developed and widely used techniques have been employed for SARS-CoV-2 detection. Typical representatives of these analytical technologies include enzyme-linked immunosorbent assay (ELISA) [ 14 ], lateral flow assay (LFA) [ 15 ], biosensors, and isothermal amplification-assisted nucleic acid test [ 16 , 17 ]. LFA has obtained a great success in COVID-19 diagnosis because it well matches the ASSURED criteria of the WHO for POC testing [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Point-of-care COVID-19 diagnostics powered by lateral flow assay

Zhou

Ding

et al. 2021

TrAC Trends in Analytical Chemistry

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136

118

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Since its first discovery in December 2019, the global coronavirus disease 2019 (COVID-19) pandemic caused by the novel coronavirus (SARS-CoV-2) has been posing a serious threat to human life and health. Diagnostic testing is critical for the control and management of the COVID-19 pandemic. In particular, diagnostic testing at the point of care (POC) has been widely accepted as part of the post restriction COVID-19 control strategy. Lateral flow assay (LFA) is a popular POC diagnostic platform that plays an important role in controlling the COVID-19 pandemic in industrialized countries and resource-limited settings. Numerous pioneering studies on the design and development of diverse LFA-based diagnostic technologies for the rapid diagnosis of COVID-19 have been done and reported by researchers. Hundreds of LFA-based diagnostic prototypes have sprung up, some of which have been developed into commercial test kits for the rapid diagnosis of COVID-19. In this review, we summarize the crucial role of rapid diagnostic tests using LFA in targeting SARS-CoV-2-specific RNA, antibodies, antigens, and whole virus. Then, we discuss the design principle and working mechanisms of these available LFA methods, emphasizing their clinical diagnostic efficiency. Ultimately, we elaborate the challenges of current LFA diagnostics for COVID-19 and highlight the need for continuous improvement in rapid diagnostic tests.

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Direct competitive ELISA enhanced by dynamic light scattering for the ultrasensitive detection of aflatoxin B1 in corn samples

Cited by 51 publications

References 37 publications

Recent Advances in Conventional Methods and Electrochemical Aptasensors for Mycotoxin Detection

Recent Advances in Conventional Methods and Electrochemical Aptasensors for Mycotoxin Detection

Advances in Colorimetric Strategies for Mycotoxins Detection: Toward Rapid Industrial Monitoring

Point-of-care COVID-19 diagnostics powered by lateral flow assay

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