Direct Construction of a Three-dimensional Structure with Cells and Hydrogel

Yan, Yongnian; Wang, Xiaohong; Xiong, Zhuo; Liu, Haixia; Liu, Feng; Lin, Feng; Wu, Rendong; Zhang, Renji; Lu, Qiuyu

doi:10.1177/0883911505053658

Cited by 138 publications

(113 citation statements)

References 12 publications

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“…Among the studies related to bioprinting, Yan and co-workers [25][26][27] have attempted to print 3D scaffolds from alginate-gelatin blends. Here, we elaborate this approach with particular attention paid to the ink properties required for effective printing with 10 respect to both the delivery and integrity of structure formed.…”

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confidence: 99%

Bio-ink properties and printability for extrusion printing living cells

et al. 2013

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Additive biofabrication (3D bioprinting) makes it possible to create scaffolds with precise geometries, control over pore interconnectivity and architectures that are not possible with conventional techniques. Inclusion of cells within the ink to form a "bio-ink" presents the potential to print 3D structures that can be implanted into damaged/diseased tissue to promote highly controlled cell-based regeneration and repair. The properties of an 'ink' are defined by its formulation and critically influence the delivery and integrity of structure formed. Importantly, the ink properties need to conform to biological requirements necessary for the cell system that they are intended to support and it is often challenging to find conditions for printing that facilitate this critical aspect of tissue bioengineering. In this study, alginate (Alg) was selected as the major component of the 'bio-ink' formulations for extrusion printing of cells. The rheological properties of alginate-gelatin (AlgGel) blends were compared with pre-crosslinked alginate and alginate solution to establish their printability whilst maintaining their ability to support optimal cell growth. Pre-crosslinked alginate on its own was liquidlike during printing. However, by controlling the temperature, Alg-Gel formulations had higher viscosity, storage modulus and consistency which facilitated higher print resolution/precision. Compression and indentation testing were used to examine the mechanical properties of alginate compared to Alg-Gel. Both types of gels yielded similar results with modulus increasing with alginate concentration. Decay in mechanical properties over time suggests that Alg-Gel slowly degrades in cell culture media with more than 60% decrease in initial modulus over 7 days. The viability of primary myoblasts delivered as a myoblast/Alg-Gel bio-ink was not affected by the printing process, indicating that the Alg-Gel matrix provides a potential means to print 3D constructs that may find application in myoregenerative applications. control over pore interconnectivity and architectures that are not possible with conventional techniques. Inclusion of cells within the ink to form a "bio-ink" presents the potential to print 3D structures that can be implanted into damaged/diseased tissue to promote highly controlled cell-based regeneration and repair. The properties of an 'ink' are defined by its formulation and critically influence the delivery and integrity of structure formed. Importantly, the ink properties need to conform to biological requirements 10 necessary for the cell system that they are intended to support and it is often challenging to find conditions for printing that facilitate this critical aspect of tissue bioengineering. In this study, alginate (Alg) was selected as the major component of the 'bio-ink' formulations for extrusion printing of cells. The rheological properties of alginate-gelatin (Alg-Gel) blends were compared with pre-crosslinked alginate and alginate solution to establish their printability whil...

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confidence: 99%

Bio-ink properties and printability for extrusion printing living cells

et al. 2013

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“…Extrusion-based bioprinting is a particular deposition process using fluidic polymeric solutions or hydrogels as bioinks ( Figure 2) [18][19][20][21][22][23][24] . The extrusion-based bioprinters are normally consisted of a three-axis automatic extrusion system equipped with a fluid-dispensing nozzle (or head) [25][26][27][28] .…”

Section: Extrusion-based Bioprintingmentioning

confidence: 99%

Progress in organ 3D bioprinting

Fan¹,

Liu²,

Chen³

et al. 2024

IJB

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Three dimensional (3D) printing is a hot topic in today's scientific, technological and commercial areas. It is recognized as the main field which promotes "the Third Industrial Revolution". Recently, human organ 3D bioprinting has been put forward into equity market as a concept stock and attracted a lot of attention. A large number of outstanding scientists have flung themselves into this field and made some remarkable headways. Nevertheless, organ 3D bioprinting is a sophisticated manufacture procedure which needs profound scientific/technological backgrounds/knowledges to accomplish. Especially, large organ 3D bioprinting encounters enormous difficulties and challenges. One of them is to build implantable branched vascular networks in a predefined 3D construct. At present, organ 3D bioprinting still in its infancy and a great deal of work needs to be done. Here we briefly overview some of the achievements of 3D bioprinting technologies in large organ, such as the bone, liver, heart, cartilage and skin, manufacturing.Keywords: organ; 3D bioprinting; bone; heart; liver; cartilage; skin

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“…5%, 10%, 15%, 20%), dextran-40 (i.e. 5%, 10%, 15%, 20%) or the combination of the glycerol and dextran-40 were fabricated at 4 o C in 20 min using a cell-assembling machine as described previously [12][13][14][15][16][17][18][19][20][21].…”

Section: Fabrication and Freezing-thawing Proceduresmentioning

confidence: 99%

Cryopreservation of cell/hydrogel constructs based on a new cell-assembling technique

Wang

Yan

et al. 2009

Nature Precedings

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Organ manufacturing products hold the promise to be used widely in the future for complex organ failings. The cryopreservation of the product is a very important step in the commercialization activities. In this article, a new cell cryopreservation technique, whereby, cryoprotectants were directly incorporated into the cell/hydrogel constructs, prototyped according to the predesigned structure and then subjected to a special freezing/thawing process. The rheological and hydration properties of the cryopreservation systems indicated that the hydratabilities of the gelatin/alginate hydrogels were greatly increased while the eutectic temperatures were greatly decreased by the addition of glycerol. Dextran-40 was found to be effective to improve the cell survival when incorporated with glycerol. The optimal volume concentration of the

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Direct Construction of a Three-dimensional Structure with Cells and Hydrogel

Cited by 138 publications

References 12 publications

Bio-ink properties and printability for extrusion printing living cells

Bio-ink properties and printability for extrusion printing living cells

Progress in organ 3D bioprinting

Cryopreservation of cell/hydrogel constructs based on a new cell-assembling technique

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