Direct detection of potato leafroll virus in potato tubers by immunocapture and the isothermal nucleic acid amplification method NASBA

Leone, G.; Schijndel, Harm B. van; Gemen, Bob van; Schoen, C.D.

doi:10.1016/s0166-0934(97)02203-9

Cited by 34 publications

(18 citation statements)

References 10 publications

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“…Real-time NASBA has been applied to detect plant viruses including Strawberry vein banding virus (SVBV), Apple stem pitting virus (ASPV) and PPV (Klerks et al, 2001;Leone et al, 1997;Olmos et al, 2007;Vaskova et al, 2004).…”

Section: Isothermal Amplificationmentioning

confidence: 99%

A Review of Detection Methods for the Plant Viruses

Jeong¹,

Ju²,

Noh³

2014

Research in Plant Disease

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The early and accurate detection of plant viruses is an essential component to control those. Because the globalization of trade by free trade agreement (FTA) and the rapid climate change promote the country-tocountry transfer of viruses and their hosts and vectors, diagnosis of viral diseases is getting more important. Because symptoms of viral diseases are not distinct with great variety and are confused with those of abiotic stresses, symptomatic diagnosis may not be appropriate. From the last three decades, enzyme-linked immunosorbent assays (ELISAs), developed based on serological principle, have been widely used. However, ELISAs to detect plant viruses decrease due to some limitations such as availability of antibody for target virus, cost to produce antibody, requirement of large volume of sample, and time to complete ELISAs. Many advanced techniques allow overcoming demerits of ELISAs. Since the polymerase chain reaction (PCR) developed as a technique to amplify target DNA, PCR evolved to many variants with greater sensitivity than ELISAs. Many systems of plant virus detection are reviewed here, which includes immunological-based detection system, PCR techniques, and hybridization-based methods such as microarray. Some of techniques have been used in practical, while some are still under developing to get the level of confidence for actual use.

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Section: Isothermal Amplificationmentioning

confidence: 99%

A Review of Detection Methods for the Plant Viruses

Jeong¹,

Ju²,

Noh³

2014

Research in Plant Disease

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“…These include various isothermal amplification methods, such as nucleic acid sequence-based amplification (NASBA) (Leone et al 1997); helicase-dependent isothermal amplification (Vincent et al 2004); exponential nucleic acid amplification reaction (Tan et al 2005); and methods based on rolling circle amplification (Yi et al 2006;Zhang et al 2006), all of which have the benefit of not requiring complex thermal cycling equipment, while retaining the specificity and sensitivity advantages of PCR-based methods. Such methods also have the potential to be combined with novel detection strategies such as the use of functionalised gold nanoparticles (Tan et al 2005), bioluminescence (Gandelman et al 2006), or biosensors (JaffrezicRenault et al 2007), which could be less costly than the fluorescent detection required for real-time PCR.…”

Section: Future Technologiesmentioning

confidence: 99%

“…In the 1990s this work mainly focused on molecular methods; during this time, a range of different potato virus assays was designed including assays based on conventional PCR (Spiegel and Martin 1993;Mumford et al 1994), the ligase chain reaction (O'Donnell et al 1996), NASBA (Klerks et al 2001;Leone et al 1997), and real-time PCR (Schoen et al 1996;Boonham et al 2000). While these and other reports demonstrated that such methods could be used for the sensitive detection of potato viruses direct from tubers, it was also clear that much more work was required to turn this early progress into a useable, routine diagnostic service.…”

Section: Direct Tuber Testingmentioning

confidence: 99%

Exploiting generic platform technologies for the detection and identification of plant pathogens

Boonham¹,

Glover²,

Tomlinson³

et al. 2008

Eur J Plant Pathol

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The detection and identification of plant pathogens currently relies upon a very diverse range of techniques and skills, from traditional culturing and taxonomic skills to modern molecular-based methods. The wide range of methods employed reflects the great diversity of plant pathogens and the hosts they infect. The well-documented decline in taxonomic expertise, along with the need to develop ever more rapid and sensitive diagnostic methods has provided an impetus to develop technologies that are both generic and able to complement traditional skills and techniques. Realtime polymerase chain reaction (PCR) is emerging as one such generic platform technology and one that is well suited to high-throughput detection of a limited number of known target pathogens. Real-time PCR is now exploited as a front line diagnostic screening tool in human health, animal health, homeland security, biosecurity as well as plant health. Progress with developing generic techniques for plant pathogen identification, particularly of unknown samples, has been less rapid. Diagnostic microarrays and direct nucleic acid sequencing (de novo sequencing) both have potential as generic methods for the identification of unknown plant pathogens but are unlikely to be suitable as high-throughput detection techniques. This paper will review the application of generic technologies in the routine laboratory as well as highlighting some new techniques and the trend towards multidisciplinary studies.

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“…Single-strand oligoprobes that reconform upon binding to the target [94][95][96]. The fluorescence of internally quenched fluorophore is restored by reconforming, and detected by fluorescence resonance energy transfer.…”

Section: Molecular Beaconsmentioning

confidence: 99%

A commented dictionary of techniques for genotyping

Drábek

2001

Electrophoresis

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Several tools, differing in their technical and practical parameters, are available for the detection of point mutations as well as small deletions and insertions. In this article, a dictionary featuring over fifty methods for detection of mutation is presented. The distinguishing principle for each method is briefly explained. Sorting of and discussion on the methods give the reader a brief introduction to the field of genotyping.

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Direct detection of potato leafroll virus in potato tubers by immunocapture and the isothermal nucleic acid amplification method NASBA

Cited by 34 publications

References 10 publications

A Review of Detection Methods for the Plant Viruses

A Review of Detection Methods for the Plant Viruses

Exploiting generic platform technologies for the detection and identification of plant pathogens

A commented dictionary of techniques for genotyping

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