2001
DOI: 10.1016/s0022-0728(00)00251-5
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Direct electron transfer of cellobiose dehydrogenase from various biological origins at gold and graphite electrodes

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Cited by 69 publications
(65 citation statements)
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References 28 publications
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“…Only for one CDH adsorbed on graphite investigated so far the pH profile has shown a drastically different behavior, viz. for the enzyme from Humicola insolens, for which a six times higher DET response for cellobiose was registered at pH 7 compared with that at pH 4 [9]. To summarize, the buffer systems showed partial differences in their effect on the response.…”
Section: Response Versus Ph-profilementioning
confidence: 87%
“…Only for one CDH adsorbed on graphite investigated so far the pH profile has shown a drastically different behavior, viz. for the enzyme from Humicola insolens, for which a six times higher DET response for cellobiose was registered at pH 7 compared with that at pH 4 [9]. To summarize, the buffer systems showed partial differences in their effect on the response.…”
Section: Response Versus Ph-profilementioning
confidence: 87%
“…A imobilização empregando-se SAM permite um maior controle sobre estas duas últimas variáveis, facilitando a confecção deste tipo de sensor 38,108,124,125 . A transferência eletrônica entre o sítio ativo de um elemento biológico imobilizado e a superfície de um eletrodo é objeto de inúmeros estudos 124,[126][127][128][129] . Marcus e Sutin 130 demonstraram que a constante de velocidade da comunicação eletroquímica é governada pela diferença de potencial entre os centros redox envolvidos e, também, pela distân-cia entre o sítio ativo e a superfície do eletrodo.…”
Section: Figura 6 Representação Esquemática Dos Processos De Imobiliunclassified
“…Gorton e colaboradores 134,135 também estudaram a transferência de elétrons da enzima celubiose desidrogenase (CDH) sobre SAM. A CDH é uma enzima que contém dois sítios ativos, um grupo flavina (FAD) e um grupo heme (citocromo b).…”
Section: Figura 6 Representação Esquemática Dos Processos De Imobiliunclassified
“…However, it is interesting to note that the redox enzymes secreted by the ligninolytic white-rot fungi, viz. lignin peroxidase (LiP) [25,26], manganese peroxidase (MnP) [25], laccase [5, 6, 27 ± 29], and cellobiose dehydrogenase [18,30,31], all show efficient DET. This contrasts with extracellular redox enzymes produced by other fungi, e.g., glucose and pyranose oxidase, which do not exhibit DET.…”
Section: Introductionmentioning
confidence: 99%