Direct enzyme patterning with microcontact printing and the growth of ZnO nanoparticles on the catalytic templates at room temperature

Fabijanic, Kristina Ivana; Perez‐Castillejos, Raquel; Matsui, Hiroshi

doi:10.1039/c1jm11609f

Cited by 10 publications

(11 citation statements)

References 21 publications

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“…Patterning of submicron features with functional species is a necessary step in the fabrication of many devices such as integrated circuits, information storage devices, microelectrochemical systems (MEMS), miniaturized sensors, micro-optical components, and others [10][11][12][13][14][15]. Manipulation of inorganic-organic systems provides new possibilities for designing multifunctional (such as ferroelectric, ferromagnetic, multiferroic, dielectric properties) materials with novel cross-coupled properties [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Nanoparticle and poly(methyl methacrylate) co-dispersion in anisole

Liu

Gervasio

2015

J Mater Sci

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Aqueous nanoparticle dispersions have been widely studied for their important industrial applications, including photocatalyst, coating, and casting. However, much less work has been conducted on nanoparticle dispersions in organic solvents, especially when co-dispersing with dissolved polymers. In this study, we explore TiO 2 / ZnO nanoparticle and poly(methyl methacrylate) (PMMA) co-dispersion in organic solvent anisole. To achieve uniformly dispersed suspensions, nanoparticle surface functionalization with nonanoic acid and 1-pentanol is conducted. Nonanoic acid has higher surface coverage on TiO 2 and the two dispersants show similar surface coverage on ZnO. Heat treatment enhances the dispersant adsorption on the nanoparticle surfaces. For both TiO 2 / PMMA and ZnO/PMMA co-dispersions, higher solids loading leads to higher suspension viscosity. Higher volume fraction of nanoparticles in the nanoparticle plus PMMA solid phase results in lower viscosity, mainly by reducing the entanglement of the dissolved PMMA chains.

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Section: Introductionmentioning

confidence: 99%

Nanoparticle and poly(methyl methacrylate) co-dispersion in anisole

Liu

Gervasio

2015

J Mater Sci

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“…The ability to immobilize and pattern enzymes on a solid surface with a predefined spatial configuration is relevant for many bionanotechnological and biological applications, such as biosensing, bionanoreactors, enzyme-assisted lithography, and cell biology studies. − As the size of the bionanotechnological devices continues to scale down, the capability of fabricating enzyme patterns at the nanometer scale is crucial. Highly defined enzyme patterns at this length scale can significantly benefit the fabrication of biosensors with densely packed arrays for highly sensitive and high-throughput detection. ,, Due to the catalytic properties of enzymes, enzyme nanopatterns can also be implemented to grow inorganic materials as device building blocks or to locally degrade macromolecular films, generating templates for the subsequent patterning of miniature electrical circuits. ,, Furthermore, quasi-3D patterning on soft polymer surfaces with single enzyme resolution (<10 nm) allows a more precise engineering of the enzymes’ microenvironment, which can potentially mimic more closely the biological context and thereby enhance enzyme function and stability.…”

Section: Introductionmentioning

confidence: 99%

“…In recent decades, a variety of patterning techniques have been developed and used for fabricating enzyme patterns. Among these techniques, microcontacting printing, ,,, optical lithography, − and inkjet printing can provide enzyme patterns of micrometer size. Although enzyme patterns with sub-100 nm resolution have been generated using different methods, such as electron beam lithography (EBL), − nanoimprint lithography (NIL), dip pen nanolithography (DPN), ,, scanning probe lithography (SPL) methods, − and various self-assembly processes, − it is still an open challenge to achieve enzyme patterning using a simple and effective method which combines sub-10 nm resolution and high throughput.…”

Section: Introductionmentioning

confidence: 99%

“…Highly defined enzyme patterns at this length scale can significantly benefit the fabrication of biosensors with densely packed arrays for highly sensitive and high-throughput detection. 1,9,10 Due to the catalytic properties of enzymes, enzyme nanopatterns can also be implemented to grow inorganic materials as device building blocks or to locally degrade macromolecular films, generating templates for the subsequent patterning of miniature electrical circuits. 2,9,11 Furthermore, quasi-3D patterning on soft polymer surfaces with single enzyme resolution (<10 nm) allows a more precise engineering of the enzymes' microenvironment, which can potentially mimic more closely the biological context and thereby enhance enzyme function and stability.…”

Section: Introductionmentioning

confidence: 99%

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Sub-10 nm Resolution Patterning of Pockets for Enzyme Immobilization with Independent Density and Quasi-3D Topography Control

Liu

Kumar

Calò

et al. 2019

ACS Appl. Mater. Interfaces

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The ability to precisely control the localization of enzymes on a surface is critical for several applications including biosensing, bionanoreactors, and single molecule studies. Despite recent advances, fabrication of enzyme patterns with resolution at the single enzyme level is limited by the lack of lithography methods that combine high resolution, compatibility with soft, polymeric structures, ease of fabrication, and high throughput. Here, a method to generate enzyme nanopatterns (using thermolysin as a model system) on a polymer surface is demonstrated using thermochemical scanning probe lithography (tc-SPL). Electrostatic immobilization of negatively charged sulfonated enzymes occurs selectively at positively charged amine nanopatterns produced by thermal deprotection of amines along the side-chain of a methacrylate-based copolymer film via tc-SPL. This process occurs simultaneously with local thermal quasi-3D topographical patterning of the same polymer, offering lateral sub-10 nm resolution, and vertical 1 nm resolution, as well as high throughput (5.2 × 104 μm2/h). The obtained single-enzyme resolution patterns are characterized by atomic force microscopy (AFM) and fluorescence microscopy. The enzyme density, the surface passivation, and the quasi-3D arbitrary geometry of these patterned pockets are directly controlled during the tc-SPL process in a single step without the need of markers or masks. Other unique features of this patterning approach include the combined single-enzyme resolution over mm2 areas and the possibility of fabricating enzymes nanogradients.

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“…In this article, we report on the spatiotemporal control of nanoparticle growth via generation of concentration gradients using nanopatterned enzymes. Enzymes are involved in biomineralization and have been extensively used in bio‐inspired approaches because they can generate crystal growth precursors in situ and act as seeding points for nucleating new crystals . Here, we utilize the enzyme glucose oxidase (GOx) for growing gold nanoparticles as a model of crystallization of a functional material .…”

Section: Introductionmentioning

confidence: 99%

Nanoparticle Growth via Concentration Gradients Generated by Enzyme Nanopatterns

Rica

Bat

Herpoldt

et al. 2014

Adv Funct Materials

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Biomineralizing organisms can grow nanomaterials with unexpected morphologies in an organic matrix where temporal and vectorial gradients of crystal growth precursors are established. Here, concentration gradients for the crystallization of gold nanoparticles are generated and applied on silicon substrates. Gradients of crystal growth precursors are generated by enzymes patterned as lines that are separated by distances ranging from the micro-to the nanoscale. The concentration of crystallization precursors around the lines separated by nanometric distances is not only determined by mass transport and enzyme activity but also by the nanoscale organization of biocatalysts. This nanoscale organization favors non-classical crystal growth conditions that lead to the formation of nanoparticle clusters containing nanocrystals that are highly crystallographically aligned. The combination of bottom-up crystal growth with top-down electron beam lithography enables the fabrication of micrometric patterns containing gold nanoparticles of different size, shape, and surface density. These are all critical parameters that determine the physical properties of these nanomaterials.

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Direct enzyme patterning with microcontact printing and the growth of ZnO nanoparticles on the catalytic templates at room temperature

Cited by 10 publications

References 21 publications

Nanoparticle and poly(methyl methacrylate) co-dispersion in anisole

Nanoparticle and poly(methyl methacrylate) co-dispersion in anisole

Sub-10 nm Resolution Patterning of Pockets for Enzyme Immobilization with Independent Density and Quasi-3D Topography Control

Nanoparticle Growth via Concentration Gradients Generated by Enzyme Nanopatterns

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