Direct hapten-linked multiplexed immunoassays on polycarbonate surface

Tamarit-López, Jesús; Morais, Sergi; Puchades, Rosa; Maquieira, Ángel

doi:10.1016/j.bios.2010.08.066

Cited by 17 publications

(5 citation statements)

References 17 publications

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“…For CD/DVD based assays, the protocol should not affect either the surface or optical path of the detection system. Several groups have demonstrated methods using chemically functionalized polycarbonate surfaces of conventional CDs or DVDs to enable biomolecules binding, 26,27 where the formation of -COOH groups on the PC surface was achieved by UV. However, limited success has been achieved mainly because of the low surface density of reactive groups and strong non-specific adsorption.…”

Section: Cell Capture On a Modified Surface And Imagingmentioning

confidence: 99%

Lab-on-DVD: standard DVD drives as a novel laser scanning microscope for image based point of care diagnostics

Ramachandraiah

Amasia

Cole³

et al. 2013

Lab Chip

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We present a novel "Lab-on-DVD" system and demonstrate its capability for rapid and low-cost HIV diagnostics by counting CD4+ cells isolated from whole blood. We show that a commercial DVD drive can, with certain modifications, be turned into an improved DVD-based laser scanning microscope (DVD-LSM). The system consists of a multi-layered disposable polymer disc and a modified commercial DVD reader with rotational control for sample handling, temperature control for optimized bioassay, a photodiode array for detection, and software for signal processing and user interface - all the necessary components required for a truly integrated lab-on-a-chip system, with the capability to deliver high-resolution images down to 1 μm in size. Using discs modified with antibodies, we specifically captured CD4+ cells from whole blood, demonstrating single cell resolution imaging. The novel integrated DVD platform with sub-micron image resolution brings, for the first time, affordable cellular diagnostic testing to the point-of-care and should be readily applicable at resource-limited settings.

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Section: Cell Capture On a Modified Surface And Imagingmentioning

confidence: 99%

Lab-on-DVD: standard DVD drives as a novel laser scanning microscope for image based point of care diagnostics

Ramachandraiah

Amasia

Cole³

et al. 2013

Lab Chip

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“…In addition, several attempts have been made to use optical scanning of spinning disks as the basis for immunoassays. A group in Madrid has been developing technology based on standard disks (33)(34)(35)(36)(37)(38). In the most recent version (38 ), 6 environmentally related small molecules are determined in a competition assay with haptens directly linked to the plastic surface, and colloidal gold antibody is used for label with silver intensification.…”

Section: Optical Immunosensor Systemsmentioning

confidence: 99%

Discerning Trends in Multiplex Immunoassay Technology with Potential for Resource-Limited Settings

Gordon

Michel

2012

Clinical Chemistry

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BACKGROUND In the search for more powerful tools for diagnoses of endemic diseases in resource-limited settings, we have been analyzing technologies with potential applicability. Increasingly, the process focuses on readily accessible bodily fluids combined with increasingly powerful multiplex capabilities to unambiguously diagnose a condition without resorting to reliance on a sophisticated reference laboratory. Although these technological advances may well have important implications for the sensitive and specific detection of disease, to date their clinical utility has not been demonstrated, especially in resource-limited settings. Furthermore, many emerging technological developments are in fields of physics or engineering, which are not readily available to or intelligible to clinicians or clinical laboratory scientists. CONTENT This review provides a look at technology trends that could have applicability to high-sensitivity multiplexed immunoassays in resource-limited settings. Various technologies are explained and assessed according to potential for reaching relevant limits of cost, sensitivity, and multiplex capability. Frequently, such work is reported in technical journals not normally read by clinical scientists, and the authors make enthusiastic claims for the potential of their technology while ignoring potential pitfalls. Thus it is important to draw attention to technical hurdles that authors may not be publicizing. SUMMARY Immunochromatographic assays, optical methods including those involving waveguides, electrochemical methods, magnetorestrictive methods, and field-effect transistor methods based on nanotubes, nanowires, and nanoribbons reveal possibilities as next-generation technologies.

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“…Considerable effort has thus devoted to developing fast and accurate approaches for malathion analyses. Enzyme-linked immunosorbent assays (ELISA) (Brun et al, 2005;Tamarit-López et al, 2011) represent one of the most typical and powerful strategies among them. In ELISA, antibodies are indispensable for analyte recognition and subsequent signal reporting in ELISA for malathion detection.…”

Section: Introductionmentioning

confidence: 99%

Bio-inspired aptamers decorated gold nanoparticles enable visualized detection of malathion

Zhan

Tao

Xie

et al. 2023

Front. Bioeng. Biotechnol.

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Biosensors always respond to the targets of interest in a specific manner, employing biological or bio-mimic recognition elements such as antibodies and aptamers. Inspired by target recognition in nature, an aptamer-mediated, gold nanoparticle-based sensing approach is developed in this work for effective determination of malathion. The sensing system consists of negatively charged aptamer probes, and polycationic proteins, protamine, as well as exceptional colorimetric nanoprobes, barely gold nanoparticles (AuNPs). Protamine molecules bound to aptamer probes hinder the aggregation of AuNPs, while no such inhibition is maintained when aptamer-specific malathion is introduced into the solution, thus leading to the solution colour change from red to blue observable by the naked eye. The assay is accomplished via a mix-and-measure step within 40 min with a detection limit as low as 1.48 μg/L (3σ/s rule). The assay method also exhibits high selectivity and good applicability for the quantification of malathion in tap water with recovery rates of 98.9%–109.4%. Additionally, the good detection accuracy is also confirmed by the high-performance liquid chromatography method. Therefore, the non-enzymatic, label- and device-free characteristics make it a robust tool for malathion assay in agricultural, environmental, and medical fields.

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Direct hapten-linked multiplexed immunoassays on polycarbonate surface

Cited by 17 publications

References 17 publications

Lab-on-DVD: standard DVD drives as a novel laser scanning microscope for image based point of care diagnostics

Lab-on-DVD: standard DVD drives as a novel laser scanning microscope for image based point of care diagnostics

Discerning Trends in Multiplex Immunoassay Technology with Potential for Resource-Limited Settings

Bio-inspired aptamers decorated gold nanoparticles enable visualized detection of malathion

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