2001
DOI: 10.11150/kansenshogakuzasshi1970.75.300
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Direct Identification of Mycobacteria from the Positive Cultures in Mycobacteria Growth Indicator Tube (MGIT)

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“…These 10 strains were randomly selected to include 5 strains each from solid medium and liquid medium. For each MTC strain, the sample consisted of a 1 μL loopful of growth from solid medium, which corresponds to a mean cell density of 2.8 × 10 8 CFU/mL, or from liquid medium, which corresponds to a mean cell density of 10 4 -10 7 CFU/mL [10]. As described above, the optimized protocol consisted of ultrasonication at 18˚C for 1 minute at a setting of PIP 40 W/DF 50%.…”
Section: Mycobacterial Inactivation Using Ultrasonication Equipmentmentioning
confidence: 99%
“…These 10 strains were randomly selected to include 5 strains each from solid medium and liquid medium. For each MTC strain, the sample consisted of a 1 μL loopful of growth from solid medium, which corresponds to a mean cell density of 2.8 × 10 8 CFU/mL, or from liquid medium, which corresponds to a mean cell density of 10 4 -10 7 CFU/mL [10]. As described above, the optimized protocol consisted of ultrasonication at 18˚C for 1 minute at a setting of PIP 40 W/DF 50%.…”
Section: Mycobacterial Inactivation Using Ultrasonication Equipmentmentioning
confidence: 99%