Autoignition of a turbulent stratified mixing layer between nitrogen-diluted hydrogen and hot air under an elevated pressure of 50 atm is studied using direct numerical simulations (DNS) in this work. Homogeneous isotropic turbulence is superimposed on the flow field. Reduced chemical mechanisms and a detailed multicomponent diffusion model are employed. In addition to turbulent mixing ignition (TMI), homogeneous mixing ignition (HMI) and laminar mixing ignition (LMI) are also investigated for comparison. Autoignition chemistry over a wide range of pressures is studied in HMI and LMI, which shows different behaviors at elevated pressures versus low pressures. The importance of H 2 O 2 and HO 2 in TMI is highlighted as radical sinks during the ignition process and can also be used as an indicator for locating the ignition spots. Moreover, OH radicals can be used as a marker variable for the transition of autoignition to flame propagation under high pressure. According to the present study, turbulence has 2 some influence on the radical explosion stage especially for the conservation of H 2 O 2 under the elevated pressure of 50 atm. Autoignition kernels forming away from the most reactive mixture fraction isosurface are identified, which is a hybrid of autoignition and diffusive-ignition.