2018
DOI: 10.1016/j.fsigen.2017.10.010
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Direct PCR amplification of DNA from human bloodstains, saliva, and touch samples collected with microFLOQ ® swabs

Abstract: Previous studies have shown that nylon flocked swabs outperform traditional fiber swabs in DNA recovery due to their innovative design and lack of internal absorbent core to entrap cellular materials. The microFLOQ Direct swab, a miniaturized version of the 4N6 FLOQSwab, has a small swab head that is treated with a lysing agent which allows for direct amplification and DNA profiling from sample collection to final result in less than two hours. Additionally, the microFLOQ system subsamples only a minute portio… Show more

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Cited by 65 publications
(48 citation statements)
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“…There was not significant inhibition using nylon swabs as the average RFU results were still higher than 3,000 [4]. Previous studies have proved that direct PCR using the nylon swab can generate informative STR profiles [5,[13][14][15].…”
Section: Discussionmentioning
confidence: 85%
“…There was not significant inhibition using nylon swabs as the average RFU results were still higher than 3,000 [4]. Previous studies have proved that direct PCR using the nylon swab can generate informative STR profiles [5,[13][14][15].…”
Section: Discussionmentioning
confidence: 85%
“…This swab was conceived for oral mucosa sampling but not to collect OF, and even less to detect DOA. 11,12 To our knowledge, there is no recommendation or protocol regarding illicit drugs' detection in OF using this swab and we do not have data on the stability of DOA collected with this system.…”
Section: Introductionmentioning
confidence: 99%
“…For economic reasons, the French Authorities chose the FLOQSwabs™ kit (FS, Copan, CA, USA, marketed in France by Apri 2000, Cournon d'Auvergne, France). This swab was conceived for oral mucosa sampling but not to collect OF, and even less to detect DOA . To our knowledge, there is no recommendation or protocol regarding illicit drugs' detection in OF using this swab and we do not have data on the stability of DOA collected with this system.…”
Section: Introductionmentioning
confidence: 99%
“…Commercial products designed for direct amplification are now available, notably microFLOQ ® swabs from Copan Diagnostics. Reports of touch DNA sampling with microFLOQ swabs have been very promising [14]. We report here a trial on direct amplification of touch DNA taken from volunteers, carried out in order to evaluate microFLOQ swabs for touch DNA, and compare them with both proven and experimental direct amplification techniques.…”
Section: Introductionmentioning
confidence: 99%