Direct somatic embryogenesis from leaves, cotyledons and hypocotyls of Hippophae rhamnoides

Liu, C. Q.; Xia, Xinli; Yin, Weilun; Zhou, Jianghong; Tang, Haoru

doi:10.1007/s10535-007-0135-4

Cited by 18 publications

(15 citation statements)

References 20 publications

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“…The SEs maturation appeared to be not well synchronized, as different developmental stages of SEs were observed at the same time. Such direct development of SEs has also been observed in many other plants (Mandal and Datta 2005, Chen and Chang 2006, Liu et al 2007). Auxins and cytokinins are considered to be key factor in the acquisition of embryogenic competence in somatic embryogenesis inductions (Feher et al 2003).…”

supporting

confidence: 63%

Morpho-histological study of direct somatic embryogenesis in endangered species Frittilaria meleagris

et al. 2010

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Direct somatic embryogenesis of Frittilaria meleagris L. was induced using leaf base explants excised from in vitro grown shoots. Somatic embryos occurred at the basal part of leaf explants 4 weeks after culture on a Murashige and Skoog (MS) medium supplemented with various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) or kinetin (KIN). The highest number of somatic embryos (SEs) were formed (9.74) from leaf explant on MS medium supplemented with 0.1 mg dm -3 2,4-D after 4 weeks of culture initiation. An initial exposure to a low concentration of KIN in the medium also enhanced SEs induction. Our observations by light and scanning electron microscopy revealed that SEs originate directly from the epidermal and subepidermal layers of leaf explant. The developmental stages of somatic embryogenesis from the first unequal cell division through the meristematic clusters, multi-cellular globular somatic embryos to the fully formed cotyledonary embryos were determined. After 4 weeks on MS medium without plant growth regulators, SEs developed into bulblets.

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confidence: 63%

Morpho-histological study of direct somatic embryogenesis in endangered species Frittilaria meleagris

et al. 2010

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“…Several candidate genes have been isolated from apomictic Cenchrus and their functional validation required an efficient protocol on genetic transformation which is not available yet. Plant regeneration through somatic embryogenesis has been successfully accomplished in many plant species (recently e.g., Liu et al 2007, Junaid et al 2007, Ganesh Kumari et al 2008, Sanatombi et al 2008. Previous studies on tissue culture in buffel grass have demonstrated plant regeneration from embryogenic callus, induced from immature inflorescences (Kackar and Shekhawat 1991) as well as from immature and mature embryos (Ross et al 1995).…”

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis and regeneration of Cenchrus ciliaris genotypes from immature inflorescence explants

Yadav¹,

Jha²,

Mahalakshmi³

et al. 2009

Biologia plant.

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An efficient, highly reproducible system for plant regeneration via somatic embryogenesis was developed for Cenchrus ciliaris genotypes IG-3108 and IG-74. Explants such as seeds, shoot tip segments and immature inflorescences were cultured on Murashige and Skoog (MS) medium supplemented with 2.0 -5.0 mg dm -3 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5 mg dm -3 N 6 -benzyladenine (BA) for induction of callus. Callus could be successfully induced from all the three explants of both the genotypes. But the high frequency of embryogenic callus could be induced only from immature inflorescence explants. Somatic embryos were formed from nodular, hard and compact embryogenic calli when 2,4-D concentration was gradually reduced and BA concentration increased. Histological studies of somatic embryos indicated the presence of shoot apical meristem with leaf primordia. Ultrastructural details of globular and scutellar somatic embryos further validated successful induction and progression of somatic embryogenesis. Shoots were differentiated upon germination of somatic embryos on MS medium containing 2,4-D (0.25 mg dm -3 ) and BA or kinetin (1 -5 mg dm -3 ). Roots were induced on ½ MS medium containing charcoal (0.8 %), and the regenerated plants transferred to pots and established in the soil showed normal growth and fertility.

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“…Further, among the seedlingderived explants, cotyledon and hypocotyl explants were not as responsive as leaf explants for organogenesis and somatic embryogenesis. Similarly, Liu et al (2007a) reported that leaf, cotyledon and hypocotyls taken from the seedlings of sea buckthorn showed more or less similar response towards somatic embryogenesis.…”

Section: Factors Controlling Micropropagationmentioning

confidence: 80%

“…sinensis but the cultures died during subculturing. Similarly, Liu et al (2007a) reported that only 30-50% somatic embryos converted into normal plantlets due to the formation of several abnormal embryos during regeneration. Gupta and Singh (2003) and Singh and Gupta (2008) while working on micropropagation of Indian sea buckthorn (H. rhamnoides ssp.…”

Section: Constraints In Micropropagationmentioning

confidence: 97%

Biotechnological interventions in sea buckthorn (Hippophae L.): current status and future prospects

Kalia

Singh

Manoj

et al. 2011

Trees

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Sea buckthorn (Hippophae L., Elaeagnaceae) is an economically and ecologically important medicinal plant comprising of species which are winter hardy, dioecious, wind-pollinated multipurpose shrubs bearing yellow or orange berries with nitrogen-fixing ability. It grows widely in cold regions of Indian Himalayas, China, Russia, Europe and many other countries. It is commonly known as 'cold desert gold' due to its high potential as a bio-resource for land reclamation, reducing soil erosion and its multifarious uses. The wild populations are being used for harvesting economic benefits with negligible plantation efforts. Although this plant has many excellent traits, it is still in an early phase of domestication. This woody plant is prone to many pests and diseases which destroy the plants and halt its commercial production. Limited progress has been made for improvement of sea buckthorn through breeding programs due to long juvenile period and lack of QTL linkage map, which makes screening of mapping populations a time-consuming and labor-intensive task. Conventional propagation methods, i.e. seeds, softwood and hardwood cuttings, and suckers are in place but are cumbersome and season dependent. Therefore, application of modern tools of biotechnology needs to be standardized for harnessing maximum benefits from this nutraceutical plant. Improvement of this genus through genetic transformation requires an efficient regeneration system, which is yet to be standardized. Taxonomic status of the genus is controversial and requires more inputs. Taxonomic delineation of species and subspecies and also the breeding programs can be more robustly addressed using molecular markers. This review summarizes the progress made and suggests some future directions of research for this important fruit species.

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Direct somatic embryogenesis from leaves, cotyledons and hypocotyls of Hippophae rhamnoides

Cited by 18 publications

References 20 publications

Morpho-histological study of direct somatic embryogenesis in endangered species Frittilaria meleagris

Morpho-histological study of direct somatic embryogenesis in endangered species Frittilaria meleagris

Somatic embryogenesis and regeneration of Cenchrus ciliaris genotypes from immature inflorescence explants

Biotechnological interventions in sea buckthorn (Hippophae L.): current status and future prospects

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