Direct somatic embryogenesis from mature embryo axes in chickpea (Cicer arietinum L.)

Suhasini, K.; Sagare, Abhay P.; Krishnamurthy, K. V.

doi:10.1016/0168-9452(94)90037-x

Cited by 33 publications

(21 citation statements)

References 20 publications

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“…The maturation of normal cotyledonary embryos was 20 %, while the remaining embryos either formed callus or turned necrotic. The maturation of somatic embryos on ABA medium was reported for pigeon pea (Mohan and Krishnamurthy 2002), Phaseolus sp (Malik and Saxena 1992a), and chickpea (Suhasini et al 1994). …”

Section: Cotyledon Structure Developmentmentioning

confidence: 83%

In vitro regeneration through organogenesis and somatic embryogenesis in pigeon pea [Cajanus cajan (L.) Millsp.] cv. JKR105

Krishna

Reddy

Ramteke

et al. 2011

Physiol Mol Biol Plants

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In vitro regeneration of pigeon pea through organogenesis and somatic embryogenesis was demonstrated with pigeon pea cv. JKR105. Embryonic axes explants of pigeon pea showed greater regeneration of shoot buds on 2.5 mg L −1 6-benzylaminopurine (BAP) in the medium, followed by further elongation at lower concentrations. Rooting of shoots was observed on half-strength Murashige and Skoog (MS) medium with 2 % sucrose and 0.5 mg L −1 3-indolebutyric acid (IBA). On the other hand, the regeneration of globular embryos from cotyledon explant was faster and greater with thidiazuron (TDZ) than BAP with sucrose as carbohydrate source. These globular embryos were maturated on MS medium with abscisic acid (ABA) and finally germinated on half-strength MS medium at lower concentrations of BAP. Comparison of regeneration pathways in pigeon pea cv. JKR105 showed that the turnover of successful establishment of plants achieved through organogenesis was more compared to somatic embryogenesis, despite the production of more embryos than shoot buds.

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Section: Cotyledon Structure Developmentmentioning

confidence: 83%

In vitro regeneration through organogenesis and somatic embryogenesis in pigeon pea [Cajanus cajan (L.) Millsp.] cv. JKR105

Krishna

Reddy

Ramteke

et al. 2011

Physiol Mol Biol Plants

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“…Direct shoot organogenesis and establishment of plantlets from different explants of chickpea was reported earlier (Polisetty et al, 1996(Polisetty et al, , 1997Paul et al, 2000;Rizvi and Singh, 2000;Chauhan et al, 2003;Jayanand et al, 2003;Chakraborti et al, 2006). Plantlets were developed through direct somatic embryogenesis and through callus from different explants of chickpea (Barna and Wakhlu, 1993;Sagare et al, 1993;Suhasini et al, 1994;Kumar et al, 1994Kumar et al, , 1995Rizvi and Singh, 2000;Chauhan et al, 2002;Kar et al, 1996Kar et al, , 1997Kiran et al, 2005).…”

Section: Introductionmentioning

confidence: 95%

An optimal protocol for in vitro regeneration, efficient rooting and stable transplantation of chickpea

Anwar

Sharmila

Saradhi

2008

Physiol Mol Biol Plants

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A rapid, reproducible and efficient regeneration method was developed for chickpea (Cicer arietinum L.) using single cotyledon with half embryonal axis as explants. MS medium supplemented with 4 ìM TDZ, 10 ìM 2-iP and 2 ìM kinetin induced 50-100 adventitious buds/shoots after 14 days of culture and elongated on MS medium supplemented with 5 ìM 2-iP and 2 ìM kinetin. Healthy, strong and 100 % rooting was achieved by exposing cut ends of the shoots to 10 sec pulse treatment with 100 ìmoles/ml IBA followed by their transfer to liquid MS basal medium within 10-14 d. 2-3 cm long shoots were most suitable for rooting. Potting-mixture with good aeration and lesser capacity to retain water was most suitable for achieving successful establishment of chickpea plantlets. Garden soil mixed with sand (gravel) and bio-manure in the ratio of 1:1:1 is most suitable for achieving cent percent transplantation success. Cent percent of plantlets got acclimatized, survived in the pots and showed normal growth, development, flowering followed by podding and seeds setting. Harvesting of seeds was done after the pods were fully matured and dry. In this communication, we have demonstrated for the first time that shoot length, pulse treatment of cut ends of shoots with 100 ìmoles/ml IBA and aeration of potting mixture are key factors for rapid micro-propagation and successful establishment of chickpea.

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“…Direct somatic embryogenesis has been achieved using many explants from legumes: from plantlets of Trifolium repens, Trifolium pratense and Medicago sativa (Maheswaren and Williams, 1984); from zygotic embryos of peanut (Sellers et al, 1990) and chickpea (Hita et al, 1997) and from leaflets derived from peanut embryos (Baker and Wetzstein, 1992;Chengalrayan et al, 1994), mature chickpea embryonic axes (Suhasini et al, 1994) and leaflets (Barna and Wakhlu, 1995); intact plantlets of Phaseolus sp. (Malik and Saxena, 1992) and leaves of Psophocarpus tetragonolobus (L.) Dc plantlets (Dutta Gupta et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis and plant regeneration in Medicago arborea L.

Gallego

Hita

Villalobos

et al. 2001

In Vitro Cell.Dev.Biol.-Plant

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An efficient plant regeneration system employing cotyledons, hypocotyls, petioles and leaves as explants and characterized by continuous and prolific production of somatic embryos, has been developed with Medicago arborea ssp. arborea. The optimal somatic embryogenic response was obtained using a two-step protocol, where explants were incubated under a 16 h photoperiod for 2 mo. on Murashige and Skoog (MS) medium containing 2,4-dichlorophenoxyacetic acid (2,4-D; 9 mM) and kinetin (9 mM), and followed by transfer to kinetin-free MS medium with 2,4-D (2.25 mM). Removal of the cytokinin and a reduction in the concentration of auxin (2.25 mM) in the second step of culture were critical for enhanced production of somatic embryos. The best explants proved to be cotyledons and petioles (i.e. a mean of 18X0^0X70 somatic embryos at 3 mo. for petiole culture). Somatic embryos were converted into normal plantlets 8X00 X89% when cultured on basal MS medium with 5 mM indolebutyric acid. No somatic embryos were obtained when thidiazuron was used in the culture media. Using petioles as explants and N 6 -benzyladenine (BA), embryogenesis was induced in the second step of culture when BA was removed from the medium and the concentration of 2,4-D was decreased to 2.25 mM.

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Direct somatic embryogenesis from mature embryo axes in chickpea (Cicer arietinum L.)

Cited by 33 publications

References 20 publications

In vitro regeneration through organogenesis and somatic embryogenesis in pigeon pea [Cajanus cajan (L.) Millsp.] cv. JKR105

In vitro regeneration through organogenesis and somatic embryogenesis in pigeon pea [Cajanus cajan (L.) Millsp.] cv. JKR105

An optimal protocol for in vitro regeneration, efficient rooting and stable transplantation of chickpea

Somatic embryogenesis and plant regeneration in Medicago arborea L.

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