1998
DOI: 10.1128/jb.180.2.250-255.1998
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Direct Sulfhydrylation for Methionine Biosynthesis in Leptospira meyeri

Abstract: A gene library of the Leptospira meyeri serovar semaranga strain Veldrat S.173 DNA has been constructed in a mobilizable cosmid with inserts of up to 40 kb. It was demonstrated that a Leptospira DNA fragment carrying metYcomplemented Escherichia coli strains carrying mutations inmetB. The latter gene encodes cystathionine γ-synthase, an enzyme which catalyzes the second step of the methionine biosynthetic pathway. The metY gene is 1,304 bp long and encodes a 443-amino-acid protein with a molecular mass of 45 k… Show more

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Cited by 39 publications
(28 citation statements)
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“…Three genes have been identi¢ed for the leptospiral methionine biosynthetic pathway. The metX and metY genes catalyze the ¢rst steps of the pathway [6,7] and the metF gene corresponds to the folate branch (this study). The methionine genes are dispersed over the two chromosomes of Leptospira since metX and metY are located on CI [6,7] and metF on CII (see Section 3.1).…”
Section: Dispersion Of the Methionine Genes On The Two Leptospiral Chmentioning
confidence: 93%
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“…Three genes have been identi¢ed for the leptospiral methionine biosynthetic pathway. The metX and metY genes catalyze the ¢rst steps of the pathway [6,7] and the metF gene corresponds to the folate branch (this study). The methionine genes are dispersed over the two chromosomes of Leptospira since metX and metY are located on CI [6,7] and metF on CII (see Section 3.1).…”
Section: Dispersion Of the Methionine Genes On The Two Leptospiral Chmentioning
confidence: 93%
“…A cosmid library of L. interrogans serovar icterohaemorraghiae strain Verdun was made as described for another Leptospira species [6]. A recombinant cosmid (XG10) harboring an insert of around 40 kb in size was cleaved with the Sau3A restriction endonuclease according to the manufacturer's speci¢cations.…”
Section: Cloning Of the L Interrogans Metf Genementioning
confidence: 99%
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