Direct ultrasound-assisted extraction and characterization of phenolic compounds from fresh houseleek (Sempervivum marmoreum L.) leaves

Karabegović, Ivana; Stojičević, Saša S.; Veličković, Dragan T.; Nikolić, Nada Č.; Lazić, Miodrag L.

doi:10.2298/hemind170402017k

Cited by 6 publications

(1 citation statement)

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“…Similar studies were carried out on different Sempervivum species, showing a good antioxidant potential [17]. Karabegovic et al [24] have determined a EC50 value of 0.0246±0.45 mg/mL for a ultrasound assisted methanolic extract of S. tectorum, which highlights the high concentration of antioxidant compounds in the species, that can be recovered by more advanced extraction techniques. The authors showed that the extraction technique plays a key role in the recovery of antioxidant compounds, which is confirmed by the comparison between the antioxidant activity of the ethanolic and hydroethanolic extracts of Sempervivum ruthenicum Koch [10].…”

Section: Dpph Radical Scavenging Methodsmentioning

confidence: 53%

Phytochemical Profile and Total Antioxidant Capacity of Sempervivum ruthenicum Koch Hydroethanolic Extract

Mihai¹,

Dumitrescu²,

Raducanu³

et al. 2019

Rev. Chim.

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The scope of this work is a phytochemical analysis and antioxidant activity assay of a Sempervivum ruthenicum Koch hydroethanolic extract. The hydroethanolic extract was prepared from the dried leaves of the plant by maceration in a water and methanol mixture (50:50 v/v). The total phenolic content of the extract was calculated to be 3.0501�0.0272 mg/mL and the total flavonoid content was determined in a concentration of 3.113�0.0394 mg/mL. The HPLC-DAD analysis revealed phenolic acids and flavonoids, which were quantified. The most prevalent phenolic acids in the extracts were gallic and ellagic acids, with concentrations of 1.2443�0.0475 mg/mL, respectively 0.6339�0.0026 mg/mL. The heteroside astragalin was present in high concentration of 1.1934�0.0754 mg/mL. The DPPH free radical scavenging assay revealed the EC50 value of the extract to be 2.5788�0.003 mg/mL. These results suggest a good scavenging ability of the extract, which is due to the abundance of polyphenolic compounds. The antioxidant activity of the extract demonstrates a high scavenging ability at low doses. Sempervivum ruthenicum Koch shows a promising phytochemical profile that suggests it�s use in pathological conditions that involve high oxidative stress.

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Section: Dpph Radical Scavenging Methodsmentioning

confidence: 53%