Direct visual detection of DNA based on the light scattering of silica nanoparticles on a human papillomavirus DNA chip

Piao, Jing Yu; Park, Eun Hee; Choi, Kihwan; Quan, Bo; Kang, Do Hyung; Park, Pan Yun; Kim, Dai Sik; Chung, Doo Soo

doi:10.1016/j.talanta.2009.08.024

Cited by 10 publications

(8 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The authors demonstrated the development of such a portable nanodevice for detection and genotyping of HPV. [14] The lower limit of detection was 100 pM with a simple microscope illumination system, and 200 pM to 1 nM with the naked eye. The advantages include the low cost of the light-scattering detector system and the ability to use the system without the device by nakedeye examination; also, plain silica particles are readily available.…”

Section: Sexually Transmitted Infectionsmentioning

confidence: 97%

“…A DNA microarray chip assay based on light-scattering of aggregated silica nanoparticle probes has been developed. [14] Here, target HPV DNA is sandwiched between the capture DNA immobilized on the chip and the probe DNA immobilized on the plain silica nanoparticle. The spots where the sandwich reaction occurs appear bright white and are readily distinguishable with the naked eye.…”

Section: Sexually Transmitted Infectionsmentioning

confidence: 99%

See 1 more Smart Citation

Molecular and Nanotechnologic Approaches to Etiologic Diagnosis of Infectious Syndromes

et al. 2011

View full text Add to dashboard Cite

Infectious diseases are a major global public health problem. Multiple agents are now recognized to cause indistinguishable illnesses. The term 'syndrome' applies to such situations, for which early and rapid diagnosis of the infecting agent would enable prompt and appropriate therapy. Public health physicians would also get timely information on the specific etiology of the infectious syndrome, facilitating intervention at the community level in the face of outbreaks or epidemics. A variety of molecular techniques have been evaluated for rapid diagnosis of infectious syndromes. These techniques include real-time multiplex PCR, DNA microarray, loop-mediated isothermal amplification, and other similar assays. This review surveys such state-of-the-art technologies.

show abstract

Section: Sexually Transmitted Infectionsmentioning

confidence: 97%

Section: Sexually Transmitted Infectionsmentioning

confidence: 99%

Molecular and Nanotechnologic Approaches to Etiologic Diagnosis of Infectious Syndromes

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Another DNA chip was demonstrated to detect HPV-16 based on light scattering of silica nanoparticles, which were used as labels [54]. After sample DNA was hybridized with DNA capture probes and immobilized on the DNA chip, silica particles covered with sequences complementary to target DNA were incubated.…”

Section: A Optical Detection Technologiesmentioning

confidence: 99%

Advances in Nanotechnology and Microfluidics for Human Papillomavirus Diagnostics

et al. 2015

View full text Add to dashboard Cite

| Human papillomavirus (HPV) has been shown in many studies as a prerequisite for the development of cervical cancer, which is the second most common and predominant form of malignancy among women worldwide, with 270 000 deaths (80% of whom live in developing countries) and 500 000 new cases per year. Early diagnosis of cervical cancer allows patients to be fully treated. Therefore, there is high clinical utility of HPV diagnostics even with binary positive/ negative indication of the presence of HPV in patient samples.Although the Pap smear is widely used for cervical cancer screening, this method still suffers from low sensitivity and specificity. Thus, simple, rapid and inexpensive diagnostic methods are needed to detect the etiology of cervical cancer, i.e., HPV, especially high-risk oncogenic subtypes 16 and 18.Here, we review the existing assays and platforms employed for HPV diagnosis, and highlight recent advances in nanotechnology and microfluidics that potentially enable new approaches for HPV diagnosis.

show abstract

“…These nanostructures were found to be suitable labels for the microarrays on glass slides. The principle of light scattering by silica nanoparticles was used to develop a simple DNA chip with visual detection [140]. Silica particles were used as labels for the probes, which, during sandwich hybridization, form a dendrimer-like network of particles which increase markedly the aggregation degree of the particles.…”

Section: Commercial Hpv Testsmentioning

confidence: 99%

DNA Microarrays for Pathogen Detection

Schulze

Rubtsova

Bachmann

2015

Modern Techniques for Pathogen Detection

View full text Add to dashboard Cite

Direct visual detection of DNA based on the light scattering of silica nanoparticles on a human papillomavirus DNA chip

Cited by 10 publications

References 28 publications

Molecular and Nanotechnologic Approaches to Etiologic Diagnosis of Infectious Syndromes

Molecular and Nanotechnologic Approaches to Etiologic Diagnosis of Infectious Syndromes

Advances in Nanotechnology and Microfluidics for Human Papillomavirus Diagnostics

DNA Microarrays for Pathogen Detection

Contact Info

Product

Resources

About