Directed evolution of the B. subtilis nitroreductase YfkO improves activation of the PET-capable probe SN33623 and CB1954 prodrug

Fixing Flavins: Hijacking a Flavin Transferase for Equipping Flavoproteins with a Covalent Flavin Cofactor

“…Nitroreductases are considered valuable biotechnological tools. They are intensely studied as prodrug-activating enzymes acting on nitro-containing compounds, enabling the treatment of cancer and infections . They are also considered to be valuable biocatalysts for producing amines .…”