Directional Bleb Formation in Spherical Cells under Temperature Gradient

Oyama, Kotaro; Arai, Tomomi; Isaka, Akira; Sekiguchi, Taku; Itoh, Hideki; Seto, Yusuke; Miyazaki, Makito; Itabashi, Takeshi; Ohki, Takashi; Suzuki, Madoka; Ishiwata, Shin’ichi

doi:10.1016/j.bpj.2015.06.016

Cited by 26 publications

(34 citation statements)

References 68 publications

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“…Microheating induces convection water flow 15 16 . Therefore, we investigated whether the convection flow itself induced neurite outgrowth by subjecting neurons to an artificial water flow prior to microheating ( Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Here, we focus on the use of a laser beam to generate microscopic temperature increases. Localized microscopic heating (and subsequent cooling) induces gene expression 11 , muscle contraction 12 13 14 , directional bleb formation 15 , and cellular excitations such as Ca 2+ bursts 16 17 and the induction of transmembrane electrical currents 18 19 20 21 . Temperature increases also accelerate cytoskeletal polymerization and molecular motor activities.…”

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confidence: 99%

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Triggering of high-speed neurite outgrowth using an optical microheater

Oyama

Zeeb

Kawamura

et al. 2015

Sci Rep

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Optical microheating is a powerful non-invasive method for manipulating biological functions such as gene expression, muscle contraction, and cell excitation. Here, we demonstrate its potential usage for regulating neurite outgrowth. We found that optical microheating with a water-absorbable 1,455-nm laser beam triggers directional and explosive neurite outgrowth and branching in rat hippocampal neurons. The focused laser beam under a microscope rapidly increases the local temperature from 36 °C to 41 °C (stabilized within 2 s), resulting in the elongation of neurites by more than 10 μm within 1 min. This high-speed, persistent elongation of neurites was suppressed by inhibitors of both microtubule and actin polymerization, indicating that the thermosensitive dynamics of these cytoskeletons play crucial roles in this heat-induced neurite outgrowth. Furthermore, we showed that microheating induced the regrowth of injured neurites and the interconnection of neurites. These results demonstrate the efficacy of optical microheating methods for the construction of arbitrary neural networks.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Triggering of high-speed neurite outgrowth using an optical microheater

Oyama

Zeeb

Kawamura

et al. 2015

Sci Rep

Self Cite

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“…In Fig. 2a , a membrane in a ‘bag'-like shape can be observed behind the stably trapped nucleus, this deformation is caused by the drag force that the blood exerts and resembles the blebs that form due to local heating of cells 20 . This experiment shows that in the zebrafish optical trapping of erythrocytes is strong and seems more robust than that achieved in mouse ear 8 , where trapping had to be done gradually while making crucial use of the wall of the blood vessel.…”

Section: Resultsmentioning

confidence: 99%

Optical micromanipulation of nanoparticles and cells inside living zebrafish

et al. 2016

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Regulation of biological processes is often based on physical interactions between cells and their microenvironment. To unravel how and where interactions occur, micromanipulation methods can be used that offer high-precision control over the duration, position and magnitude of interactions. However, lacking an in vivo system, micromanipulation has generally been done with cells in vitro, which may not reflect the complex in vivo situation inside multicellular organisms. Here using optical tweezers we demonstrate micromanipulation throughout the transparent zebrafish embryo. We show that different cells, as well as injected nanoparticles and bacteria can be trapped and that adhesion properties and membrane deformation of endothelium and macrophages can be analysed. This non-invasive micromanipulation inside a whole-organism gives direct insights into cell interactions that are not accessible using existing approaches. Potential applications include screening of nanoparticle-cell interactions for cancer therapy or tissue invasion studies in cancer and infection biology.

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“…Microscopic temperature imaging in solution can be performed by relatively simple methods that detect the thermal quenching of water-soluble luminescent dyes such as rhodamine B (Ross et al 2001), BCECF (Braun and Libchaber 2002), and tetramethylrhodamine or Alexa Fluor 555 conjugated to dextran (Oyama et al 2015a). Luminescent nanosheets containing the temperature-sensitive dye europium (III) thenoyltrifluoroacetonate trihydrate (Eu-TTA) visualize the temperature distribution on the glass surface during optical microheating of solution (Itoh et al 2014;Oyama et al 2020).…”

Section: Microscopic Temperature Measurementmentioning

confidence: 99%

Opto-thermal technologies for microscopic analysis of cellular temperature-sensing systems

2021

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Could enzymatic activities and their cooperative functions act as cellular temperature-sensing systems? This review introduces recent opto-thermal technologies for microscopic analyses of various types of cellular temperature-sensing system. Optical microheating technologies have been developed for local and rapid temperature manipulations at the cellular level. Advanced luminescent thermometers visualize the dynamics of cellular local temperature in space and time during microheating. An optical heater and thermometer can be combined into one smart nanomaterial that demonstrates hybrid function. These technologies have revealed a variety of cellular responses to spatial and temporal changes in temperature. Spatial temperature gradients cause asymmetric deformations during mitosis and neurite outgrowth. Rapid changes in temperature causes imbalance of intracellular Ca2+ homeostasis and membrane potential. Among those responses, heat-induced muscle contractions are highlighted. It is also demonstrated that the short-term heating hyperactivates molecular motors to exceed their maximal activities at optimal temperatures. We discuss future prospects for opto-thermal manipulation of cellular functions and contributions to obtain a deeper understanding of the mechanisms of cellular temperature-sensing systems.

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Directional Bleb Formation in Spherical Cells under Temperature Gradient

Cited by 26 publications

References 68 publications

Triggering of high-speed neurite outgrowth using an optical microheater

Triggering of high-speed neurite outgrowth using an optical microheater

Optical micromanipulation of nanoparticles and cells inside living zebrafish

Opto-thermal technologies for microscopic analysis of cellular temperature-sensing systems

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