Directional gene-transfer into the brain by an adenoviral vector tagged with magnetic nanoparticles

Hashimoto, Mitsuhiro; Hisano, Yu

doi:10.1016/j.jneumeth.2010.10.027

Cited by 26 publications

(18 citation statements)

References 15 publications

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“…Thus, a RAd vector tagged with MNPs was ICV injected in mouse embryos in vitro and in vivo. By applying an external magnetic field to a limited area of the head of the embryos, transgene delivery was restricted to that region [65]. The same route could be exploited to implement minimally invasive therapeutic gene delivery in the adult rodent brain by ICV administration of MNP-viral vector complexes at distal sites and subsequent magnetic trapping of the complexes at the target brain region by means of a properly focused external magnetic field.…”

Section: Neurological Diseasesmentioning

confidence: 99%

Magnetic Field-Assisted Gene Delivery: Achievements and Therapeutic Potential

Schwerdt

Goya²,

Calatayud³

et al. 2012

CGT

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The discovery in the early 2000's that magnetic nanoparticles (MNPs) complexed to nonviral or viral vectors can, in the presence of an external magnetic field, greatly enhance gene transfer into cells has raised much interest. This technique, called magnetofection, was initially developed mainly to improve gene transfer in cell cultures, a simpler and more easily controllable scenario than in vivo models. These studies provided evidence for some unique capabilities of magnetofection. Progressively, the interest in magnetofection expanded to its application in animal models and led to the association of this technique with another technology, magnetic drug targeting (MDT). This combination offers the possibility to develop more efficient and less invasive gene therapy strategies for a number of major pathologies like cancer, neurodegeneration and myocardial infarction. The goal of MDT is to concentrate MNPs functionalized with therapeutic drugs, in target areas of the body by means of properly focused external magnetic fields. The availability of stable, nontoxic MNP-gene vector complexes now offers the opportunity to develop magnetic gene targeting (MGT), a variant of MDT in which the gene coding for a therapeutic molecule, rather than the molecule itself, is delivered to a therapeutic target area in the body. This article will first outline the principle of magnetofection, subsequently describing the properties of the magnetic fields and MNPs used in this technique. Next, it will review the results achieved by magnetofection in cell cultures. Last, the potential of MGT for implementing minimally invasive gene therapy will be discussed.

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Section: Neurological Diseasesmentioning

confidence: 99%

Magnetic Field-Assisted Gene Delivery: Achievements and Therapeutic Potential

Schwerdt

Goya²,

Calatayud³

et al. 2012

CGT

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“…To achieve this, some investigators selected coating agents, such as chitosan. 16 In 1995, Mumper et al 17 were the first group to propose the use of chitosan as a DNA carrier. It showed significantly lower toxicity than poly-l-lysine and polyethylenimine.…”

Section: Alvizo-baez Et Almentioning

confidence: 99%

Systemic delivery and activation of the <em>TRAIL</em> gene in lungs, with magnetic nanoparticles of chitosan controlled by an external magnetic field

Alvizo-Báez

Luna-Cruz

Vilches-Cisneros

et al. 2016

IJN

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“…PEI, a cationic polymer, is widely used for nucleic acid transfection [87–89] and also serves as a nanoparticle dispersant [90]. PEI-coated MNPs enhance transfection efficiency [38,41,42,44–46,48,49,51,54,55]. …”

Section: Gene Deliverymentioning

confidence: 99%

“…We reasoned, therefore, that a non-viral gene delivery system using iron oxide-based MNPs could provide a powerful tool for next-generation therapies. Gene delivery using MNPs has been successful for delivering nucleic acids into living cells with high efficiency and low cytotoxicity [38,41,42,44–46,48,49,51,54,55]. Currently, there are several methods for inducing cellular differentiation.…”

Section: Cell Transplantation Therapy Using Mnpsmentioning

confidence: 99%

Application of Magnetic Nanoparticles to Gene Delivery

Kami

Takeda

Ikoma

et al. 2011

IJMS

135

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Nanoparticle technology is being incorporated into many areas of molecular science and biomedicine. Because nanoparticles are small enough to enter almost all areas of the body, including the circulatory system and cells, they have been and continue to be exploited for basic biomedical research as well as clinical diagnostic and therapeutic applications. For example, nanoparticles hold great promise for enabling gene therapy to reach its full potential by facilitating targeted delivery of DNA into tissues and cells. Substantial progress has been made in binding DNA to nanoparticles and controlling the behavior of these complexes. In this article, we review research on binding DNAs to nanoparticles as well as our latest study on non-viral gene delivery using polyethylenimine-coated magnetic nanoparticles.

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Directional gene-transfer into the brain by an adenoviral vector tagged with magnetic nanoparticles

Cited by 26 publications

References 15 publications

Magnetic Field-Assisted Gene Delivery: Achievements and Therapeutic Potential

Magnetic Field-Assisted Gene Delivery: Achievements and Therapeutic Potential

Systemic delivery and activation of the <em>TRAIL</em> gene in lungs, with magnetic nanoparticles of chitosan controlled by an external magnetic field

Application of Magnetic Nanoparticles to Gene Delivery

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