1985
DOI: 10.1038/bjc.1985.13
|View full text |Cite
|
Sign up to set email alerts
|

Disaggregation of human solid tumours by combined mechanical and enzymatic methods

Abstract: Summary Two combined mechanical and enzymatic disaggregation techniques and a simple mechanical disaggregation procedure were compared. The combined procedures involved a mechanical comminution of the tumour tissue followed by incubation in trypsin. In one method, the tissue was subjected to long-term trypsinization at 40C, and in the other procedure, repeated short-term trypsinization at 37°C was applied. The results were compared in terms of the yield of viable cells, plating efficiency, the ability to produ… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3

Citation Types

0
10
0

Year Published

1986
1986
2017
2017

Publication Types

Select...
8

Relationship

0
8

Authors

Journals

citations
Cited by 25 publications
(10 citation statements)
references
References 21 publications
0
10
0
Order By: Relevance
“…Trypan blue exclusion has previously been used following combined mechanical and enzymatic dissociation methods (tissue minced, incubated 4°C with 0.5% trypsin 12–20 h, then agitated with rotating razor blades for 5–15 min) on solid tumors’ (small-cell lung carcinomas and testicular cancer) preculture yielding 1.5–5 × 10 4 viable cells/mg [40], thus our experimental system appears to extend the range from that previously reported.…”
Section: Discussionmentioning
confidence: 99%
“…Trypan blue exclusion has previously been used following combined mechanical and enzymatic dissociation methods (tissue minced, incubated 4°C with 0.5% trypsin 12–20 h, then agitated with rotating razor blades for 5–15 min) on solid tumors’ (small-cell lung carcinomas and testicular cancer) preculture yielding 1.5–5 × 10 4 viable cells/mg [40], thus our experimental system appears to extend the range from that previously reported.…”
Section: Discussionmentioning
confidence: 99%
“…Cells with a greater colony-forming ability are obtained via enzymatic disaggregation when the enzyme is treated at 4 8C for a long period. 43 Furthermore, enzymatically disaggregated cells are capable of multilineage differentiation. 9,23 However, enzymatic disaggregation is associated with cytotoxicity and is more expensive than the outgrowth method.…”
Section: Discussionmentioning
confidence: 99%
“…The technical need for about lo6 cells in each sample for flow cytometry may prove to be a limitation to the use of the assay, especially if it is applied on tumor tissue obtained directly from patients where large biopsies are not always available (7). Furthermore, the in vitro sensitivity assay may produce false-positive results in cases where drug-induced cell cycle perturbation is not accompanied by cell kill (10,17), and false-negative results with drugs that kill cells equally in all parts of the cell cycle.…”
Section: Discussionmentioning
confidence: 99%
“…Three heterotransplanted human small-cell carcinomas of the lung (SCCL) were selected for this study because of their different sensitivities to melphalan (9). The tumors were disaggregated by a combined mechanical and enzymatic method (7).…”
mentioning
confidence: 99%