Discovery and mapping of two new rust resistance genes, R17 and R18, in sunflower using genotyping by sequencing

Qi, Lili; Talukder, Zahirul I.; J., G.; Li, X. H.

doi:10.1007/s00122-021-03826-x

Cited by 9 publications

(14 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Rust resistance in sunflower is controlled by single dominant genes. To date, a total of 17 rust resistance genes, R 1 – R 5 , R 10 – R 12 , R 13a , R 13b , R 14 – R 18 , P u6 and R adv, have been reported in sunflower, and 15 of them were mapped to various regions across the sunflower genome: chromosome 2 ( R 5 ); chromosome 8 ( R 1 and R 15 ); chromosome 11 ( R 12 and R 14 ); chromosome 13 ( R 4 , R 11 , R 13a , R 13b , R 16 – R 18 , P u6 , and R adv ); and chromosome 14 ( R 2 ) ( Supplementary Table S2 ) [ 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 ].…”

Section: Introductionmentioning

confidence: 99%

Genetic Insight into Disease Resistance Gene Clusters by Using Sequencing-Based Fine Mapping in Sunflower (Helianthus annuus L.)

Song

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

Rust and downy mildew (DM) are two important sunflower diseases that lead to significant yield losses globally. The use of resistant hybrids to control rust and DM in sunflower has a long history. The rust resistance genes, R13a and R16, were previously mapped to a 3.4 Mb region at the lower end of sunflower chromosome 13, while the DM resistance gene, Pl33, was previously mapped to a 4.2 Mb region located at the upper end of chromosome 4. High-resolution fine mapping was conducted using whole genome sequencing of HA-R6 (R13a) and TX16R (R16 and Pl33) and large segregated populations. R13a and R16 were fine mapped to a 0.48 cM region in chromosome 13 corresponding to a 790 kb physical interval on the XRQr1.0 genome assembly. Four disease defense-related genes with nucleotide-binding leucine-rich repeat (NLR) motifs were found in this region from XRQr1.0 gene annotation as candidate genes for R13a and R16. Pl33 was fine mapped to a 0.04 cM region in chromosome 4 corresponding to a 63 kb physical interval. One NLR gene, HanXRQChr04g0095641, was predicted as the candidate gene for Pl33. The diagnostic SNP markers developed for each gene in the current study will facilitate marker-assisted selections of resistance genes in sunflower breeding programs.

show abstract

Section: Introductionmentioning

confidence: 99%

Genetic Insight into Disease Resistance Gene Clusters by Using Sequencing-Based Fine Mapping in Sunflower (Helianthus annuus L.)

Song

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, the emergence of novel pathogen virulence through either sexual or asexual reproduction has rendered most of the existing rust‐resistant inbred lines and commercial hybrids susceptible to rust, which could lead to epidemics when weather conditions are conducive for disease development (Markell et al., 2009; Gong, Gulya, et al., 2013). Among 17 rust R genes, only 7, R 11 , R 12 , R 13a , R 13b , and R 14 – R 16 , remain effectively resistant to all P. helianthi races identified in North America so far (Gong, Gulya, et al., 2013; Ma et al., 2018; Qi et al., 2021). Therefore, it is necessary to discover novel rust resistance genes for breeding, and ultimately, long‐term management of sunflower rust.…”

Section: Introductionmentioning

confidence: 99%

“…GP‐378, PI 698198), developed through pedigree selection from the introduced South African lines. The two lines harbor the rust resistance genes, R 17 and R 18 , respectively (Qi et al., 2021), and have resistance to a wide range of P. helianthi races, including the newly emerged race 777 and the predominant race 336, providing new sources to expand sunflower rust resistant gene pools.…”

Section: Introductionmentioning

confidence: 99%

Registration of two oilseed sunflower germplasms, HA‐R18 and HA‐R19, resistant to sunflower rust

Seiler

2022

J of Plant Registrations

Self Cite

View full text Add to dashboard Cite

Sunflower rust, incited by the fungus Puccinia helianthi Schwein., is a serious fungal disease in the sunflower (Helianthus annuus L.) growing areas worldwide, with an increasing importance in North America in recent years due to the frequent evolution of new pathogen races. HA‐R18 (Reg. no. GP‐377, PI 698197) and HA‐R19 (Reg. no. GP‐378, PI 698198) are rust‐resistant germplasm lines that were developed and released cooperatively in 2020 by the USDA‐ARS, Sunflower and Plant Biology Research Unit and the Agricultural Experiment Station of North Dakota State University. The rust resistance in HA‐R18 and HA‐R19 originated from KP193 and KP199, respectively, which were identified segregating for rust resistance after screening of 58 sunflower lines introduced from South Africa. Rust evaluation of HA‐R18 and HA‐R19 with 11 P. helianthi races indicated that both lines exhibited resistance to the most rust races tested including the most predominant and the most virulent races currently identified in the United States. HA‐R18 and HA‐R19 possess the new rust resistance genes R17 and R18, respectively, both of which have been mapped to sunflower chromosome 13. The two new lines will diversify the pool of rust resistance genes and should serve as useful sources of rust resistance in sunflower.

show abstract

“…Resistance to rust is controlled by race-specific single dominant genes. Since the first two rust resistance genes (R genes), R 1 and R 2 , were reported in 1963, an additional 15 rust R gene loci (R 3 -R 5 , R 10 -R 12 , R 13a , R 13b , R 14 -R 18 , R adv , and P u6 ) have been identified in cultivated and wild sunflower (for review, see Qi et al, 2021). Rust R genes have frequently been deployed singly in sunflower production, and as a consequence, the commercial life of resistant hybrids is quickly challenged as new P. helianthi races with increased virulence evolve (Gulya et al, 2019;Harveson et al, 2016;Meyer et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…In the early 1960s, four North American races of P. helianthi were identified, while in 2012, 29 North American P. helianthi races were identified when 238 single-pustule derived isolates collected from seven U.S. states and one Canadian province were analyzed (Friskop et al, 2015). Of the 17 rust R genes identified in sunflower, only seven, R 11 in the HA-R9 line, R 12 (RHA 464), R 13a (HA-R6), R 13b (RHA 397), R 14 (PH3), R 15 (HA-R8), and R 16 (TX16R) remain effective against all known races of P. helianthi in the United States (Gong et al, 2013b;Qi et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Registration of HA‐R14, HA‐R15, HA‐R16, and HA‐R17 oilseed sunflower germplasm with broad resistance to rust and downy mildew

Seiler

2021

J of Plant Registrations

Self Cite

View full text Add to dashboard Cite

Rust and downy mildew (DM) are two of the most important sunflower diseases in the world. The development of sunflower hybrids with enhanced levels of genetic resistance to both diseases is a goal for sunflower breeding. Four oilseed sunflower (Helianthus annuus L.) germplasm lines, HA-R14 (Reg. no. GP-371, PI 698193), HA-R15 (Reg. no. GP-372, PI 698194), HA-R16 (Reg. no. GP-373, PI 698195), and HA-R17 (Reg. no. GP-374, PI 698196) are multiple rust-and DM-resistant lines that were developed and released by the USDA-ARS Sunflower and Plant Biology Research Unit in collaboration with the North Dakota Agricultural Experiment Station in January 2020. Three lines, HA-R14

show abstract

Discovery and mapping of two new rust resistance genes, R17 and R18, in sunflower using genotyping by sequencing

Cited by 9 publications

References 55 publications

Genetic Insight into Disease Resistance Gene Clusters by Using Sequencing-Based Fine Mapping in Sunflower (Helianthus annuus L.)

Genetic Insight into Disease Resistance Gene Clusters by Using Sequencing-Based Fine Mapping in Sunflower (Helianthus annuus L.)

Registration of two oilseed sunflower germplasms, HA‐R18 and HA‐R19, resistant to sunflower rust

Registration of HA‐R14, HA‐R15, HA‐R16, and HA‐R17 oilseed sunflower germplasm with broad resistance to rust and downy mildew

Contact Info

Product

Resources

About