Disease control and embryo importations

Thibier, M.; Nibart, M.

doi:10.1016/0093-691x(87)90068-9

Cited by 43 publications

(17 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In one study, more than a thousand bovine embryos were imported into France from the United States between 1983 and 1987 without any prior testing of the donors or even washing of the embryos, but no evidence of disease transmission was detected (20). Similarly, no evidence of disease transmission was reported with recent importations of many hundreds of goat embryos into Canada from South Africa, although the embryos were washed and some preexport tests on donors were done in this instance (21).…”

Section: Risks Of Cross-contaminationmentioning

confidence: 98%

Storage of cryopreserved reproductive tissues: evidence that cross-contamination of infectious agents is a negligible risk

Pomeroy

Harris

Conaghan

et al. 2010

Fertility and Sterility

View full text Add to dashboard Cite

Section: Risks Of Cross-contaminationmentioning

confidence: 98%

Storage of cryopreserved reproductive tissues: evidence that cross-contamination of infectious agents is a negligible risk

Pomeroy

Harris

Conaghan

et al. 2010

Fertility and Sterility

View full text Add to dashboard Cite

“…Nonetheless, there is a risk that viral particles may be trapped on the outer layers of the ZP. One way to remove BoHV-1 from the surface of embryos is through a procedure using trypsin baths, which was firstly reported by Nibart (1987), and has been recommended by the International ET Society -IETS (Stringfellow, 1998). However, neither washing nor trypsin treatment seem to completely remove the virus from embryos (Edens et al, 2003;D'angelo et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

In vitro interaction of bovine herpesvirus 1 with uterine tube epithelial cells and oocytes

Gonçalves

Soares²,

Orlandi

et al. 2016

Arq. Inst. Biol.

View full text Add to dashboard Cite

The aims of this study were to assess in vitro if bovine oocytes and oviductal epithelial cells from slaughterhouses for in vitro fertilization use may be infected with bovine herpesvirus 1; to analyze whether the treatment with trypsin according to the International Embryo Transfer Society guideline is efficient to inactivate the bovine herpesvirus 1; to morphologically study the virus-oocyte interaction through optical microscopy. In this study, Madin Darby Bovine Kidney (MDBK) cells that were co-cultured with oocytes matured in vitro and exposed to bovine herpesvirus 1 showed a cytopathic effect. The nested polymerase chain reaction for the supernatant was positive for the bovine herpesvirus 1, thus suggesting that the cytopathic effect observed in the MDBK monolayer was seen due to virus replication and not because of any culture toxicity. It was also observed cytopathic effect and positive nested polymerase chain reaction in MDBK cells co-cultured with in vitro maturated oocytes free of virus, but that were co-cultured in uterine epithelial cells pre-infected with bovine herpesvirus 1 and washed or not with trypsin, demonstrating an oocyte contamination by the virus. When trypsin-washing efficacy was evaluated, we could observe that the trypsin treatment was not able to eliminate the bovine herpesvirus 1 of the oocytes, and it was not observed any morphological difference in the infected oocytes.

show abstract

“…embryos had been collected were positive for antibodies [55]. Moreover, it has been shown that all viral particles are eliminated after the IETS washing procedures with trypsin [53].…”

Section: Bovine Spongiform Encephalopathy (Bse)mentioning

confidence: 99%