Disinfection and sterilisation practices in laboratories of a research institution in Uganda

Musoke, David; Mwebesa, Theodora Mondo T.; Oryema-Lalobo, Michael; Musoke, Miph Boses

doi:10.1016/j.jhin.2010.11.009

Cited by 1 publication

(2 citation statements)

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“…This proved as resistant to the CuO-SiO 2 coatings as the MRSA although the reduction was equivalent to a 99.8% reduction after 24 h. Preliminary tests with extended incubation showed a log 10 reduction of >5 after 48 h for MRSA and VRE. VRE have been shown to be capable of persisting in the environment for months and even years (Neely and Maley, 2000; Wagenvoort et al ., 2011). The use of antimicrobial coatings in a healthcare setting should help to reduce this persistence.…”

Section: Discussionmentioning

confidence: 99%

“…There is a higher risk of acquiring a HCAI if the previous room occupant had such an infection (Shaughnessy et al ., 2011). Pathogens can survive on surfaces for prolonged periods of time depending on the organism and environmental conditions (Kramer et al ., 2006; Neely and Maley, 2000; Wagenvoort et al ., 2011) and can be transmitted to hands from the environment (Bhalla et al ., 2004). Enhanced environmental cleaning has been shown to reduce rates of infection (Dancer et al ., 2009; Kochar et al ., 2009; Carling et al ., 2010; Kleypas et al .…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial activity of novel nanostructured Cu-SiO2 coatings prepared by chemical vapour deposition against hospital related pathogens

et al. 2013

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There is increasing recognition that the healthcare environment acts as an important reservoir for transmission of healthcare acquired infections (HCAI). One method of reducing environmental contamination would be use of antimicrobial materials. The antimicrobial activity of thin silica-copper films prepared by chemical vapour deposition was evaluated against standard strains of bacteria used for disinfectant testing and bacteria of current interest in HCAI. The structure of the coatings was determined using Scanning Electron Microscopy and their hardness and adhesion to the substrate determined. Antimicrobial activity was tested using a method based on BS ISO 22196:2007. The coatings had a pale green-brown colour and had a similar hardness to steel. SEM showed nano-structured aggregates of Cu within a silica matrix. A log10 reduction in viability of >5 could be obtained within 4 h for the disinfectant test strains and within 6 h for producing Acinetobacter baumannii, Klebsiella pneumoniae and Stenotrophomonas maltophilia. Activity against the other hospital isolates was slower but still gave log10 reduction factors of >5 for extended spectrum β-lactamase producing Escherichia coli and >3 for vancomycin resistant Enterococcus faecium, methicillin resistant Staphylococcus aureus and Pseudomonas aeruginosa within 24 h. The results demonstrate the importance of testing antimicrobial materials destined for healthcare use against isolates of current interest in hospitals as well as standard test strains. The coatings used here can also be applied to substrates such as metals and ceramics and have potential applications where reduction of microbial environmental contamination is desirable.

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Section: Discussionmentioning

confidence: 99%