Disk abalone (Haliotis discus discus) expresses a novel antistasin-like serine protease inhibitor: Molecular cloning and immune response against bacterial infection

Nikapitiya, Chamilani; Zoysa, Mahanama De; Oh, Chulhong; Lee, Jehee; Ekanayake, Prashani Mudika; Whang, Ilson; Choi, Cheol Young; Lee, Jae‐Seong; Umasuthan, Navaneethaiyer

doi:10.1016/j.fsi.2009.12.027

Cited by 20 publications

(14 citation statements)

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“…The inhibitory effect of CsSP-1 against PMSF confirms that the purified CsSP-1 enzyme was a serine protease. Similar results were reported from F. chinensis (Shi et al 2008), Aspergillus clavatus ES1 (Hajji et al 2007) and Haliotis discus discus (Nikapitiya et al 2010). The enzyme activity in various pH buffer solutions and at various temperatures showed that the CsSP-1 enzyme had the highest activity at pH 7.5 and 35…”

Section: Discussionsupporting

confidence: 84%

Striped murrel S1 family serine protease: immune characterization, antibacterial property and enzyme activities

et al. 2014

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Abstract:We report a molecular characterization of S1 family serine protease (SP-1) from snakehead murrel (or called striped murrel) Channa striatus (Cs). CsSP-1 polypeptide contained a catalytic core domain (otherwise known as serine protease trypsin domain) between H 20 and I 237 along with a catalytic triad at H 61 , D 104 and S 197 . Phylogenetic analysis confirmed that CsSP-1 belongs to serine protease S1 family. The tertiary structure showed that CsSP-1 contains 14 β-sheets as 2 separate β-barrels (the first β-barrel consists of 8 β-sheets in the N-terminal region and the second β-barrel consists of 6 β-sheets in the C-terminal region) and 3 α-helical regions. Significantly (P < 0.05) the highest CsSP-1 mRNA expression was observed in intestine, liver and kidney, moderate expression was seen in spleen, head kidney, skin and blood, and the lowest one in brain, gill, muscle and heart. Further, the expression was induced in intestine with fungus Aphanomyces invadans and bacteria Aeromonas hydrophila. The recombinant CsSP-1 protein showed antibacterial activity against both gram-negative and gram-positive bacteria. The optimum CsSP-1 enzyme activity against the substrate casein was determined at 8 mM casein concentration. Moreover, the activity was highly influenced by 5 mM phenyl-methylsulfonyl fluoride followed by ethylenediaminetetraacetic acid, 4-(2-aminoethyl)benzenesulfonylfluoride hydrochloride and calpain inhibitor I. The CsSP-1 enzyme exhibited the highest activity at pH 7.5 and temperature 35• C. The overall results showed the potential involvement of CsSP-1 in the immune system of murrels. However, further research is necessary to study the mechanism of implicit trypsin association in the defence process.

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Section: Discussionsupporting

confidence: 84%

Striped murrel S1 family serine protease: immune characterization, antibacterial property and enzyme activities

et al. 2014

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“…The latter include trypsin-, meprin- and papain-like proteinases, cathepsins, several serine-type endopeptidases and a large family of putative metalloproteases characterized by the ShK domain [62]. The high expression of protease inhibitors, such as antistasin, targeting trypsin [63], and Kazal-type serine-protease inhibitors [64], is also in line with the strong production of the aforementioned proteolytic enzymes, whose functional dysregulation could lead to serious cellular and tissue damage. Altogether, while some of these annotations are likely linked to extracellular digestive processes, others clearly point towards lysosomes, which are found in high numbers in vacuolated digestive cells.…”

Section: Resultsmentioning

confidence: 99%

The purplish bifurcate mussel Mytilisepta virgata gene expression atlas reveals a remarkable tissue functional specialization

et al. 2017

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Background Mytilisepta virgata is a marine mussel commonly found along the coasts of Japan. Although this species has been the subject of occasional studies concerning its ecological role, growth and reproduction, it has been so far almost completely neglected from a genetic and molecular point of view. In the present study we present a high quality de novo assembled transcriptome of the Japanese purplish mussel, which represents the first publicly available collection of expressed sequences for this species.ResultsThe assembled transcriptome comprises almost 50,000 contigs, with a N50 statistics of ~1 kilobase and a high estimated completeness based on the rate of BUSCOs identified, standing as one of the most exhaustive sequence resources available for mytiloid bivalves to date. Overall this data, accompanied by gene expression profiles from gills, digestive gland, mantle rim, foot and posterior adductor muscle, presents an accurate snapshot of the great functional specialization of these five tissues in adult mussels.ConclusionsWe highlight that one of the most striking features of the M. virgata transcriptome is the high abundance and diversification of lectin-like transcripts, which pertain to different gene families and appear to be expressed in particular in the digestive gland and in the gills. Therefore, these two tissues might be selected as preferential targets for the isolation of molecules with interesting carbohydrate-binding properties.In addition, by molecular phylogenomics, we provide solid evidence in support of the classification of M. virgata within the Brachidontinae subfamily. This result is in agreement with the previously proposed hypothesis that the morphological features traditionally used to group Mytilisepta spp. and Septifer spp. within the same clade are inappropriate due to homoplasy.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-4012-z) contains supplementary material, which is available to authorized users.

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“…The gene SERP coding for Serine protease inhibitor or serpins (which is part of a large family of enzymes that inhibit the activity of proteases) showed high expression levels in both albino conditions, in the pooled analysis. A limited number of serpins proteins has been reported in bivalves or mollusks and so far they are thought to be involved in an immune defensive role against invasive pathogens [ 94 , 96 ]. The gene coding for a PDZ domain protein ( PDZ ) showed significantly higher expression levels in black phenotypes than in albino phenotypes, both in the pooled and individual analyses.…”

Section: Discussionmentioning

confidence: 99%

Identification of genes associated with shell color in the black-lipped pearl oyster, Pinctada margaritifera

Lemer

Saulnier²,

Gueguen

et al. 2015

BMC Genomics

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BackgroundColor polymorphism in the nacre of pteriomorphian bivalves is of great interest for the pearl culture industry. The nacreous layer of the Polynesian black-lipped pearl oyster Pinctada margaritifera exhibits a large array of color variation among individuals including reflections of blue, green, yellow and pink in all possible gradients. Although the heritability of nacre color variation patterns has been demonstrated by experimental crossing, little is known about the genes involved in these patterns. In this study, we identify a set of genes differentially expressed among extreme color phenotypes of P. margaritifera using a suppressive and subtractive hybridization (SSH) method comparing black phenotypes with full and half albino individuals.ResultsOut of the 358 and 346 expressed sequence tags (ESTs) obtained by conducting two SSH libraries respectively, the expression patterns of 37 genes were tested with a real-time quantitative PCR (RT-qPCR) approach by pooling five individuals of each phenotype. The expression of 11 genes was subsequently estimated for each individual in order to detect inter-individual variation. Our results suggest that the color of the nacre is partially under the influence of genes involved in the biomineralization of the calcitic layer. A few genes involved in the formation of the aragonite tablets of the nacre layer and in the biosynthesis chain of melanin also showed differential expression patterns. Finally, high variability in gene expression levels were observed within the black phenotypes.ConclusionsOur results revealed that three main genetic processes were involved in color polymorphisms: the biomineralization of the nacreous and calcitic layers and the synthesis of pigments such as melanin, suggesting that color polymorphism takes place at different levels in the shell structure. The high variability of gene expression found within black phenotypes suggests that the present work should serve as a basis for future studies exploring more thoroughly the expression patterns of candidate genes within black phenotypes with different dominant iridescent colors.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1776-x) contains supplementary material, which is available to authorized users.

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Disk abalone (Haliotis discus discus) expresses a novel antistasin-like serine protease inhibitor: Molecular cloning and immune response against bacterial infection

Cited by 20 publications

References 46 publications

Striped murrel S1 family serine protease: immune characterization, antibacterial property and enzyme activities

Striped murrel S1 family serine protease: immune characterization, antibacterial property and enzyme activities

The purplish bifurcate mussel Mytilisepta virgata gene expression atlas reveals a remarkable tissue functional specialization

Identification of genes associated with shell color in the black-lipped pearl oyster, Pinctada margaritifera

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