Dispersive Liquid–Liquid Microextraction for the Determination of Salivary Melatonin as a Biomarker of Circadian Rhythm

Pourhossein, Mehran; Shahtaheri, Seyed Jamaled­din; Mazloumi, Adel; Rahimiforoushani, Abbas; Helmi-Kohneshahri, M.; Khani, Homa Maleck

doi:10.1134/s106193481810009x

Cited by 12 publications

(5 citation statements)

References 22 publications

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“…Another derivative of LLE is dispersive liquid–liquid microextraction (DLLME). Pourhossein et al [ 36 ] developed DLLME for the extraction of MT from saliva samples. DLLME was selected due to its rapidity, cost‐effectiveness, simple use, and low solvent consumption.…”

Section: Extraction Methodsmentioning

confidence: 99%

“…The systematic study concluded that pH 7, 2000 μl of acetonitrile as the dispersion solvent, 200 μl of CCl 4 as the extraction solvent, and 3 min of extraction time yielded the highest recovery efficiency for the determination of MT in saliva samples. [ 36 ] Furthermore, the application of heat and pressure can speed up the segregation between MT and the matrix in samples; an example is MAE, which is used for the extraction of MT from food like rice grains. MAE uses heat to separate compounds from each other in a mixture based on their different radiation absorption abilities.…”

Section: Extraction Methodsmentioning

confidence: 99%

“…Another derivative of LLE is dispersive liquid-liquid microextraction (DLLME). Pourhossein et al [36] developed DLLME for the extraction of MT from saliva samples. DLLME was selected due to its of extraction time yielded the highest recovery efficiency for the determination of MT in saliva samples.…”

Section: Introductionmentioning

confidence: 99%

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Up‐to‐date studies regarding the determination of melatonin by chromatographic methods

Alessa

Saber

Althakafy

2021

Archiv der Pharmazie

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Melatonin is an indolic compound that has been reported in the literature to exist in human-based samples, vertebrates, vegetables, fruits, and pharmaceutical products.Melatonin is considered a dietary supplement and can regulate circadian rhythms, although it has not been classified as a drug by the US Food and Drug Administration. Several analytical methods have been used for its detection. This study aimed to summarize the recent outcomes of the chromatographic methods such as electrophoretic methods, gas chromatography, and liquid chromatography, which have been used for the determination of melatonin in the last three decades, with the focus on gas chromatography and high-performance liquid chromatography methods.

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Section: Extraction Methodsmentioning

confidence: 99%

Section: Extraction Methodsmentioning

confidence: 99%

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Up‐to‐date studies regarding the determination of melatonin by chromatographic methods

Alessa

Saber

Althakafy

2021

Archiv der Pharmazie

View full text Add to dashboard Cite

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“…Although by manual injection of the extractor solvent into the sample, the tiny droplets and cloudy phase are rapidly created, the contact surface between the aqueous phase of the sample solution and the organic phase of the extractor solvent is not in its maximum range [14].…”

Section: Introductionmentioning

confidence: 99%

Ultrasound-assisted Emulsified Microextraction Followed by High Performance Liquid Chromatography for Permethrin

Pourhossein¹,

Golaghaei²,

Khaghani³

et al. 2021

J. Sib. Fed. Chem.

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Ultrasound assisted emulsification microextraction (UAEME) as a new sample preparation method was optimized for the determination of permethrin in urine samples. Also, deep eutectic solvent was used as the extracting solvent instead of organic solvents in this method. In order to determine the optimal values, six main parameters were studied in different levels. Totally, 105 runs were performed using the one-variable-at-a-time method followed by high-performance liquid chromatography with a UV detector. Under the optimum conditions, the calibration curve for permethrin was linear in the concentration range of 5 to 500 μgL‑1 for urine samples. The accuracy and reproducibility of the introduced method were determined using the relative recovery (RR%) and relative standard deviation (RSD%) tests on the fortified urine samples. RR% and RSD% were found to be 96.3–101.7 % and 3.2–7.6 %, respectively. The limit of quantification and also the limit of detection were obtained 5 and 1 μg/L, respectively. In the present study, the DES-UA-EME technique was successfully developed for the extraction of permethrin from urine samples and subsequent quantification by high-performance liquid chromatography.In comparison to the other sample preparation methods, the proposed technique has the advantages of shorter extraction time, simplicity, and applicability in laboratories with less equipment

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“…MT can be detected in the blood, urine and saliva [46,47]. There is no doubt that saliva sampling is the better choice because it is non-invasive, non-painful and easy to obtain [48].…”

Section: Introductionmentioning

confidence: 99%

Simple Colorimetric and Fluorometric Assay Based on 2,3-Naphthalenedialdehyde for Melatonin in Human Saliva

et al. 2019

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In this paper, 2,3-naphthalenedialdehyde (2,3-Nda) was selected as color reagent with colorimetric and fluorometric assay for detecting melatonin in human saliva. 2,3-naphthalenedialdehyde reacted with melatonin under the catalysis of hydrochloric acid and Fe 3+ , which the color change was observed sensitively from colorless to yellow with the naked eye. On the other hand, the product of the reaction had a large conjugate structure with strong fluorescence for the fluorometric analysis. Under optimized experimental conditions, a concentration range of melatonin was 2.5-37.5 µM (R 2 = 0.998) by colorimetry with the limit of detection for 1.288 µM (S/N = 3), and 0.01-0.1 µM (R 2 = 0.997) by fluorometry with the limit of detection for 0.004 µM (S/N = 3). The two assays were successfully applied to the determination of melatonin in human saliva, which average recoveries were 97.80% and 95.96%, respectively, which has the potential in fast clinical examination of melatonin in human saliva.

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Dispersive Liquid–Liquid Microextraction for the Determination of Salivary Melatonin as a Biomarker of Circadian Rhythm

Cited by 12 publications

References 22 publications

Up‐to‐date studies regarding the determination of melatonin by chromatographic methods

Up‐to‐date studies regarding the determination of melatonin by chromatographic methods

Ultrasound-assisted Emulsified Microextraction Followed by High Performance Liquid Chromatography for Permethrin

Simple Colorimetric and Fluorometric Assay Based on 2,3-Naphthalenedialdehyde for Melatonin in Human Saliva

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