Dispersive mixing and intraparticle partitioning of protein in size-exclusion chromatographic refolding

Oxidative protein refolding on size exclusion chromatography at high loading concentrations: Fundamental studies and mathematical modeling

“…Existing work has also developed mathematical models for separation in SEC using various model proteins in their native forms [10,11]. For protein refolding in SEC (reaction-separation SEC), the refolding reaction is incorporated into the mathematical model as well as interactions of aggregating species in case they are formed under the operating conditions being studied and the model parameters must be obtained for kinetic species formed during these reactions [5,12]. Development of an experimentally verified and robust model helps minimizing the number of screening for a broader range of operation condition and is essential for systematic process optimization.…”