Disposable amperometric magneto-immunosensor for direct detection of tetracyclines antibiotics residues in milk

Conzuelo, Felipe; Gamella, María; Campuzano, Susana; Reviejo, A.J.; Pingarrón, José M.

doi:10.1016/j.aca.2012.05.051

Cited by 114 publications

(58 citation statements)

References 24 publications

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“…Moreover, they provide a large surface area for attachment of biorecognition elements and can then be easily separated from the liquid phase by a magnet, and spread immediately to zoom out which results highly appropriate in sensor and biosensor applications 16,17,22 . MNPs also provide a favorable microenvironment for electrochemical devices where enzymes may exchange electrons directly with the transducer, improving the sensitivity and selectivity of electrochemical biosensors [24][25][26][27][28][29] . Phenolic compounds constitute a large group of organic pollutants which should be monitored in environmental engineering 30,31 .…”

Section: Introductionmentioning

confidence: 99%

Amperometric magnetobiosensors using poly(dopamine)-modified Fe₃O₄ magnetic nanoparticles for the detection of phenolic compounds

et al. 2015

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aThe synthesis of poly(dopamine)-modified magnetic nanoparticles (MNPs) and their application to prepare electrochemical enzyme biosensor useful to detect phenolic compounds is reported in this work. MNPs of about 16 nm were synthetized by co-precipitation method and conveniently modified with poly(dopamine). Non-modified and modified MNPs were characterized using X-ray photoelectron spectroscopy (XPS), Raman and infrared spectroscopy, X-ray diffraction (XRD) and atomic force microscopy (AFM). Horseradish peroxidase (HRP) was covalently immobilized onto the surface of the poly(dopamine)-modified MNPs via Michael addition and/or Schiff base formation and used to construct a biosensor for phenolic compounds by capturing the HRP-modified-nanoparticles onto the surface of a magnetic-modified glassy carbon electrode (GCE). Cyclic voltammetry and amperometry were used to study the electrochemical and analytical properties of the biosensor using hydroquinone (HQ) as redox probe. Among different phenolic compounds studied the biosensor exhibited higher sensitivity for HQ, 1.38 A M −1 cm −2, with limits of detection and quantification of 0.3 and 1.86 µM, respectively. The analytical biosensor performance for HQ and 2-aminophenol compared advantageously with previous phenolic biosensors reported in the literature.

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Section: Introductionmentioning

confidence: 99%

Amperometric magnetobiosensors using poly(dopamine)-modified Fe₃O₄ magnetic nanoparticles for the detection of phenolic compounds

et al. 2015

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“…The SPCE was positioned on a homemade dock with a magnet matching the size of the working electrode to reproducibly concentrate the MBs and a 45 mL drop of the modified MBs suspension was pipetted on the working electrode [8]. To perform the amperometric measurement, the docked chip (with the modified MBs captured on the working electrode surface) was connected to the potentiostat and immersed into a gently stirred glass electrochemical cell (see reference [8] for details) containing 10 mL of 0.05 M phosphate buffer pH 6.0 and 1.0 mM HQ (prepared just before the electrochemical measurement).…”

Section: Amperometric Measurementsmentioning

confidence: 99%

“…Nowadays, the use of magnetic beads (MBs) are known to be a powerful tool in the development of electrochemical immunosensors avoiding the need for applying laborious protocols for electrochemical substrate modification and improving their performance in terms of sensitivity, reduced assay time and minimization of matrix effects which is essential for the analysis in complex matrices such as food extracts [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Sensitive and selective magnetoimmunosensing platform for determination of the food allergen Ara h 1

Montiel

Campuzano

Pellicanò

et al. 2015

Analytica Chimica Acta

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“…Limits of detection were in the low ppb level for four TC antibiotics in untreated milk samples. The analytical usefulness of the magneto-immunosensor was demonstrated by analyzing spiked UHT whole milk and a reference milk containing a certified oxytetracycline (OTC) content [138].…”

Section: Electrochemical Affinity Biosensors For Drug Residuesmentioning

confidence: 99%

Electrochemical Affinity Biosensors in Food Safety

2017

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Safety and quality are key issues of today's food industry. Since the food chain is becoming more and more complex, powerful analytical methods are required to verify the performance of food safety and quality systems. Indeed, such methods require high sensitivity, selectivity, ability for rapid implementation and capability of automatic screening. Electroanalytical chemistry has, for decades, played a relevant role in food safety and quality assessment, taking more and more significance over time in the solution of analytical problems. At present, the implementation of electrochemical methods in the food is evident. This is in a large part due to the relevant results obtained by combining the attractive advantages of electrochemical transduction strategies (in terms of relatively simple hardware, versatility, interface with automatic logging and feasibility of application outside the laboratory environment) with those from biosensors technology. Important examples of enzyme electrochemical biosensors are those dedicated to the determination of glucose, alcohol or cholesterol are important examples. In addition, other types of different electrochemical biosensing approaches have emerged strongly in the last years. Among these, the strategies involving affinity interactions have been shown to possess a large number of applications. Therefore, electrochemical immunosensors and DNA-based biosensors have been widely used to determine major and minor components in foodstuffs, providing sufficient data to evaluate food freshness, the quality of raw materials, or the origin of samples, as well as to determine a variety of compounds at trace levels related to food safety such as micotoxins, allergens, drugs residues or pathogen microorganisms. This review discusses some critical examples of the latest advances in this area, pointing out relevant methodologies related to the measurement techniques, including the use of nanostructured electrodes and strategies for signal amplification.

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Disposable amperometric magneto-immunosensor for direct detection of tetracyclines antibiotics residues in milk

Cited by 114 publications

References 24 publications

Amperometric magnetobiosensors using poly(dopamine)-modified Fe₃O₄ magnetic nanoparticles for the detection of phenolic compounds

Amperometric magnetobiosensors using poly(dopamine)-modified Fe₃O₄ magnetic nanoparticles for the detection of phenolic compounds

Sensitive and selective magnetoimmunosensing platform for determination of the food allergen Ara h 1

Electrochemical Affinity Biosensors in Food Safety

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Disposable amperometric magneto-immunosensor for direct detection of tetracyclines antibiotics residues in milk

Cited by 114 publications

References 24 publications

Amperometric magnetobiosensors using poly(dopamine)-modified Fe3O4 magnetic nanoparticles for the detection of phenolic compounds

Amperometric magnetobiosensors using poly(dopamine)-modified Fe3O4 magnetic nanoparticles for the detection of phenolic compounds

Sensitive and selective magnetoimmunosensing platform for determination of the food allergen Ara h 1

Electrochemical Affinity Biosensors in Food Safety

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Product

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Amperometric magnetobiosensors using poly(dopamine)-modified Fe₃O₄ magnetic nanoparticles for the detection of phenolic compounds

Amperometric magnetobiosensors using poly(dopamine)-modified Fe₃O₄ magnetic nanoparticles for the detection of phenolic compounds