Disposable and portable aptamer functionalized impedimetric sensor for detection of kanamycin residue in milk sample

Sharma, Atul; Istamboulié, Georges; Hayat, Akhtar; Catanante, Gaëlle; Bhand, Sunil; Marty, Jean Louis

doi:10.1016/j.snb.2017.02.002

Cited by 104 publications

(41 citation statements)

References 37 publications

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“…In aptasensors, redox probe is charged oppositely to aptamer so that electrostatic repulsion makes the interface very sensitive to the shielding of aptamer charge or its compensation dye to target interactions. Such impedimetric sensors were reported for determination of other low‐molecular analytes, e. g., patulin , ochratoxin A , kanamycin , ibuprofen etc. The use of conductive polymers like PANI or poly( p ‐phenylene diamine) increases sensitivity of the detection due to pH‐ or charge dependency of underlying layer.…”

Section: Resultsmentioning

confidence: 99%

Electrochemical Aptasensor with Layer‐by‐layer Deposited Polyaniline for Aflatoxin M1 Voltammetric Determination

et al. 2019

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Mycotoxins are highly toxic metabolites of some fungi that frequently contaminate water, food and feed and hence cause several human and animal diseases. In this work, a new approach to the fast and reliable determination of aflatoxin M1 (AFM1) in water and milk has been proposed with reagent free protocol of signal measurement. For this purpose, DNA aptamer selective to AFM1 was entrapped between two thin layers of polyaniline (PANI) electrodeposited on glassy carbon electrode. The incubation of the aptasensor in the AFM1 solution results in remarkable decrease of the PANI intrinsic activity monitored by direct current voltammetry or electrochemical impedance spectroscopy. Appropriate calibration curves were linear in the range from 3 to 90 ng/L with limit of detection (LOD) 1–5 ng/L depending on the measurement mode. Mechanism of signal generation involves shielding electrostatic interactions between the PANI and aptamer in the surface layer and variation of its redox activity attributed to the emeraldine form of PANI. Selectivity of the response was proved by similar experiments with aflatoxin B1 and ochratoxin A and by comparison of the results with those obtained with non‐specific aptamer in the sensing layer. Simple protocol for milk pretreatment has been proposed for reliable detection of AFM1 on the level of its threshold limited values (20 ng/L).

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Section: Resultsmentioning

confidence: 99%

Electrochemical Aptasensor with Layer‐by‐layer Deposited Polyaniline for Aflatoxin M1 Voltammetric Determination

et al. 2019

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“…An impedimetric disposable and portable aptasensor for the detection of kanamycin was designed by Sharma et al [97]. Amino-functionalized kanamycin specific aptamers were immobilized onto the surface of the working SPCE via NH2-COOH interaction.…”

Section: Kanamycinmentioning

confidence: 99%

Aptamer-based Biosensors for Antibiotic Detection: A Review

Mehlhorn

Rahimi

Joseph

2018

Preprint

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Antibiotic resistance and accordingly their pollution because of uncontrolled usage has emerged as a serious problem in recent years. Hence, there is an increased demand to develope robust, easy, and sensitive methods for rapid evaluation of antibiotic and their residues. Among different analytical methods, the aptamer-based biosensors (aptasensors) have attracted considerable attention because of good selectivity, specificity, and sensitivity. This review gives an overview about recent developed aptasensors for antibiotic detection. The use of various aptamer assays to determine different groups of antibiotics like β-lactams, Aminoglycosides, Anthracyclines, Chloramphenicol, (Fluoro)Quinolones, Lincosamide, Tetracyclines and Sulfonamides are presented in this paper.

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“…For practical applications, the aptasensor performance was verified in spiked milk samples with recovery percentages of 96.88–100.5%. [ 44 ]. Table 1 provides insight on the analytical parameters of the electrochemical aptasensors for the detection of kanamycin reported in the literature.…”

Section: Electrochemical Biosensors For Antimicrobial Drug Residuementioning

confidence: 99%

An Overview on Recent Progress in Electrochemical Biosensors for Antimicrobial Drug Residues in Animal-Derived Food

Majdinasab

Yaqub

Rahim

et al. 2017

Sensors

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Anti-microbial drugs are widely employed for the treatment and cure of diseases in animals, promotion of animal growth, and feed efficiency. However, the scientific literature has indicated the possible presence of antimicrobial drug residues in animal-derived food, making it one of the key public concerns for food safety. Therefore, it is highly desirable to design fast and accurate methodologies to monitor antimicrobial drug residues in animal-derived food. Legislation is in place in many countries to ensure antimicrobial drug residue quantities are less than the maximum residue limits (MRL) defined on the basis of food safety. In this context, the recent years have witnessed a special interest in the field of electrochemical biosensors for food safety, based on their unique analytical features. This review article is focused on the recent progress in the domain of electrochemical biosensors to monitor antimicrobial drug residues in animal-derived food.

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Disposable and portable aptamer functionalized impedimetric sensor for detection of kanamycin residue in milk sample

Cited by 104 publications

References 37 publications

Electrochemical Aptasensor with Layer‐by‐layer Deposited Polyaniline for Aflatoxin M1 Voltammetric Determination

Electrochemical Aptasensor with Layer‐by‐layer Deposited Polyaniline for Aflatoxin M1 Voltammetric Determination

Aptamer-based Biosensors for Antibiotic Detection: A Review

An Overview on Recent Progress in Electrochemical Biosensors for Antimicrobial Drug Residues in Animal-Derived Food

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