Disposable Biosensor Based on Amidase/CeO2/GNR Modified Inkjet‐printed CNT Electrodes‐droplet Based Paracetamol Detection in Biological Fluids for “Point‐of‐care” Applications

Abstract

A disposable acetaminophen biosensor based on inkjet‐printed CNT electrodes (IJPCNT) modified with amidase/cerium dioxide@graphene nanoribbons composite was developed (ACeO2@GNR/IJPCNT). The enzyme amidase A was used for the first time as a recognition element. Inkjet‐printed CNT electrodes served as a basis for the construction of a biosensor that enables droplet detection using 5 μL sample volume. The biosensor showed high selectivity, sensitivity, a low detection limit of 0.18 μM and a wide working linear range from 1 to 100 μM. The proposed approach allows fast and reliable detection of acetaminophen in biological fluids with negligible matrix effect and remarkable reproducibility.

Synthesis and characterization of nanoceria for electrochemical sensing applications

“…The proposed biosensor resulted in a broad linear range of concentration between 1 and 100 μM, and a low limit of detection of 0.18 μM of acetaminophen. 127 A ratiometric electrochemical biosensor based on gold nanoparticles functionalized with cerium-based metal–organic frameworks tagged cDNA (Au@CeMOF-cDNA) have been developed for telomerase activity. CeMOFs was formed by a green method synthesized of cerium nodes), where 1,3,5-benzenetricarboxylic acid was used as a linker.…”

Inkjet-Printed Carbon Nanotube Electrodes Modified with Dimercaptosuccinic Acid-Capped Fe3O4 Nanoparticles on Reduced Graphene Oxide Nanosheets for Single-Drop Determination of Trifluoperazine

“…Disposable inkjet-printed carbon nanotube electrodes are a recently developed type of electrode with many desirable properties like high conductivity, droplet detection, small active area, and good mechanical strength of the employed CNTs, − which make them attractive for use in flexible nanoelectronic devices . After modification of the CNT patterns with other carbon-based nanocomposites, the CNT electrode performance could be further improved so that the inkjet-printed CNT electrodes have the potential to be a great starting point for the production of small flexible sensors for point-of-care (POC) electronic devices. , …”

“…29 After modification of the CNT patterns with other carbon-based nanocomposites, the CNT electrode performance could be further improved so that the inkjet-printed CNT electrodes have the potential to be a great starting point for the production of small flexible sensors for point-of-care (POC) electronic devices. 30,31 Herein, we report the fabrication and application of a sensitive and highly selective disposable droplet-based electrochemical sensor for the detection of TFP in biological fluids. Uniform iron oxide magnetic nanoparticles with a controlled core diameter of 13 nm have been synthesized by thermal decomposition of an iron oleate complex in 1-octadecene.…”